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Time for the latest @nanopore technology updates. Up next in the auditorium: an Update from the Oxford Nanopore team. #nanoporeconf Image
Please note, we invite you to read the disclaimer in the slides
CB: More information on Clive’s previous technology updates:
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Our new method for multiplexing 94 SARS-CoV-2 samples on a #Nanopore #MinION flowcell for £12.72 per sample (extracted RNA to sequencing) biorxiv.org/content/10.110…
It's a simplified lab protocol, which is faster and cheaper, utilising Nextera. We combined it with 96 #Nanopore PCR barcodes, so guppy deplexes out of the box.
We sequenced the same subset of SARS-CoV-2 samples using #Illumina, standard #Nanopore with ligation, and CoronaHiT (48 and 94 samples) with a range of Cts. All raw data in ENA, so useful on its own for comparisons, thanks to @happy_khan
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