Discover and read the best of Twitter Threads about #noise2self
Most recents (1)
Single-Cell RNA-seq data is notoriously noisy. There are dozens of methods for cleaning it, but how do you know when they’re working right?
How clean is too clean?
Presenting...Molecular Cross-Validation
biorxiv.org/content/10.110…
How clean is too clean?
Presenting...Molecular Cross-Validation
biorxiv.org/content/10.110…
Where does the noise come from? If a cell has 500k molecules and a droplet experiment nets you 5k, then you’re looking at just 1% of what was there. Lots of lowly-expressed genes will be missing by chance (the phenom formerly known as "dropout"). 
Luckily you can get a lot of cells now. Hundreds of thousands. Millions. Some of them are doing similar things to each other.
If you could somehow combine the individual sketchy pictures in the right way, you might learn better what each cell was doing.
If you could somehow combine the individual sketchy pictures in the right way, you might learn better what each cell was doing.
