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Introducing #panExM-t: a protocol where 🧠 tissue is expanded ✨24x✨, pan-stained with NHS ester, and immunolabeled.
Now you can see everything in the brain, even synaptic densities 👇. High potential for #connectomics!
bioRxiv: biorxiv.org/content/10.110… Image
Here's a data set showing synapses, mitochondria, and spine apparatuses
We found synapse morphology can be classified according to their NHS ester pan-staining patterns - similar to PTA staining in EM (Gray) Image
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