Discover and read the best of Twitter Threads about #paywall

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This @NatureMedicine paper shows 1 year after IM injection of #CRISPR AAV-SaCas9 for DMD:
-> editing event still occur 6 months after IM
-> immune response is seen within weeks & sustained
- on-target effects < 5% with AAV integration in genome

#paywall
nature.com/articles/s4159…
@NatureMedicine I have additional comments on this paper:

1/ it is great to see a year follow up after AAV injection. Important to see more of those.

2/ Immunogenicity of AAV and Cas9 are now well documented and this work confirm it. Interestingly Neonatal injection seems to overcome it
3/ Off target effects are minimal & again in line with previously reports (Table S1 & S2)

4/ Interestingly they found on-target effects with deletion, integration of AAV or increase circular RNA. Nextera technique is interesting. Would have loved to see some long read sequencing
Read 4 tweets
Our review paper on the interaction between the host innate immunity and Acinetobacter baumannii bacteria is now out ! Fei-Ju, PhD student in the lab did an amazing job going through the literature. Sadly under #paywall academic.oup.com/femspd/advance…
We reviewed all the known factors in Acinetobacter bacteria (LPS, T6SS, Omp, CPS...) that are interacting with the host and especially innate immunity and inflammasome.
Please have a look and read it if you are interested. Alternatively the paper is available here sci-hub.tw/10.1093/femspd…
Read 4 tweets
Finally the comments to Mitalipov’s group paper on #CRISPR in human embryos are out in @nature as well as the rebuttal from Ma et al. as brief communications arising #paywall. Let’s have a look more in details -> Thread
One of the major finding of the Ma et al. paper was the discovery of high frequency of inter-homologous repair mechanisms aka loss of heterozygocity LOH -> 1 allele is used as a template for
#CRISPR-HDR -> WT alleles are in fact alleles from the maternal alleles only.
We have been debating a lot on this in the last year or so. The first important comment from @Fatwa_Adikusuma et al is Ma et al. missed deleted alleles. In the paper they edited 6 loci in zygotes and EScells and checked for large deletions nature.com/articles/s4158…
Read 15 tweets

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