Discover and read the best of Twitter Threads about #phosphorylation

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In my 1st tweetorial, I’m happy to share our preprint—my first, first author manuscript! We used @10xGenomics single-nucleus RNA-seq in a mouse model of electroconvulsive seizures (ECS) to assess activity-regulated gene expression in hippocampal #neurons. biorxiv.org/content/10.110…
Activity-regulated gene expression patterns in the #hippocampus regulate #plasticity, #learning, and #memory, and are linked to risk and treatment response for neuropsychiatric disorders. However, the cell type-specificity of these patterns is still not well characterized.
We sequenced a bunch of neuronal nuclei (15,990) from mouse hippocampus and used graph-based clustering and a priori marker genes to annotate these nuclei across all major hippocampal subregions and neuron types in both of our experimental conditions (Sham and ECS). Image
Read 10 tweets
#2 B-COMPLEX/MULTI VITAMIN. Buy a liposomal or other form that increases bio-availability. Take 1 capsule w/Breakfast and Lunch. If you have stomach upset, try sublingual (under tongue) EZ Melt B-Complex in AM & Multi w/iron for Dinner. amazon.com/Liposomal-Meth…
#3 OMEGA 3s. Fish Oil, Krill Oil, Algae Oil are all good sources of Omega 3. Be careful the EPA/DHA ratio. EPA helps with inflammation/DHA helps with cognition. You need both! Opt for a 1:1 or 2:1 ratio of DHA to EPA (2:1 ratio eg. 800 DHA/400EPA) amazon.com/3Care-Concentr…
Read 93 tweets
Endothelial-derived microvesicles play a pathogenic role in vascular #inflammation, causing #endothelial barrier dysfunction by impairing adherens junctions and activating #neutrophils

More here: bit.ly/3g1FpH7
#Cardiotwitter #CardiovascularResearch

#Tweetorial below! Image
#Microvesicles (MV) are formed from the outward blebbing of the plasma membrane and carry a diverse cargo that regulate multiple processes.

During #inflammation these are produced by activated endothelial cells and released into the #circulation. TEM image of plasma MVs: (2/8) Image
In this study the authors investigated whether endothelial-derived MVs (EC-MVs) contribute to #vascular dysfunction during inflammation.

The authors used an animal model of #sepsis (CLP) and found that this increased EC-MV production (3/8) Image
Read 8 tweets

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