Discover and read the best of Twitter Threads about #plaqueassays
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How do we quantify how much #virus is in a sample (titre). We use a plaque assay, which is why he most commonly used method. Below is a graphical version I've made (on @BioRender) of how it is done in the lab.
But how do they work? - a thread
#SciComm #Virology #phdchat
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But how do they work? - a thread
#SciComm #Virology #phdchat
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Here is a photo of a #SARSCoV2 assay, the virus which causes #COVID19 ( Photo: Müller/Charité). The large areas which are clear are known as plaques which are caused by virus killing cells in a monolayer. More plaques = more virus titre
#virology #sciencetwitter
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#virology #sciencetwitter
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The technique was originally developed for #Bacteriophages bit was adapted by Dulbecco in 1952 for animal #viruses. Viral stocks are dilutes 10 fold in series and placed onto a monolayer. After incubation a gel is placed on the layer, restricting infection to nearby cells
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