Discover and read the best of Twitter Threads about #recombinases
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Happy to share my third PhD paper, published at @ACSSynBio! We developed a recombinase-based system to transfer large biosynthetic gene clusters into filamentous fungi heterologous hosts for #naturalproduct discovery. @YitHengChooi @SMS_UWA #Synbio (1/8)
doi.org/10.1021/acssyn…
doi.org/10.1021/acssyn…
We often find promising cryptic biosynthetic gene clusters (BGCs) in the genomes of fungal species that are hard to cultivate or genetically intractable. Heterologous expression in hosts such as Aspergillus nidulans is increasingly being used to access the encoded compounds.(2/8)
However, targeted integration of large BGCs remained challenging. Site-specific recombinases are well suited for the integration of large constructs but remained unadopted in filamentous fungi. So, we established a Cre/lox-based system for BGC integration in A. nidulans.🛠️🧬(3/8)
