Discover and read the best of Twitter Threads about #ribosome
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Developing an #mRNA vaccine is a sure-shot way to success? No! Despite the spectacular performance of #Pfizer & #Moderna vaccines, we also have a #CureVac fiasco. We need to dig in much deeper into the intricacies of developing a highly efficacious mRNA vaccine 1/
Super cool combination of modeling, biochemistry, and cell biology to show that co-translational topology of a membrane segment in a viral polyprotein stimulates frame shifting during translation biorxiv.org/content/10.110…. @tfmiller3 #Ribosome #Biochemistry
A weakly hydrophobic transmembrane segment picks one of two orientations - membrane insertion or not. This is stochastic, and in the cases where it passes through the membrane, the forces from the translocon “tugs” on the ribosome to cause a frameshift.
Cool combination of CGMD simulations of protein translocation and some neat labeling / conditional fluorescence experiments to establish distance between TM domain and site of frame shifting needed to stimulate force-dependent frameshift.
