Discover and read the best of Twitter Threads about #seropositive

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Our ELISA validation Breakdown for #SARSCoV2, in short, we validated six (6) ELISAs: spike & RBD IgG, IgM and IgA (check out @cispt2’s summary too!) (1/9) medrxiv.org/content/10.110… Image
Great ELISA starts with great protein: @domespo @FredNatLab generated two spike and two RBD constructs from different plasmids, and we found that @NIAIDNews VRC spike ectodomain and @SchmidtLabHMS @ragoninstitute RBD were the ideal antigens. (2/9) Image
Evaluating sensitivity & specificity of spike + RBD double positive, focusing on specificity, showed 0/100 negative controls came back “#seropositive” (Spike+RBD+) for IgG/IgM/IgA. We have since shown on robot w 0/300 for IgG (100% specificity @ 95% CI 98.78% to 100%) (3/9) Image
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