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Highlights from our #preprint on #transcription activation domains (ADs)!
-found all ADs in yeast TFs
-predicted yeast & human ADs
-tested binding of all ADs to #Mediator & #TFIID
-explained mechanism with structural modeling & kinetic experiments
(1/16)
biorxiv.org/content/10.110…
-found all ADs in yeast TFs
-predicted yeast & human ADs
-tested binding of all ADs to #Mediator & #TFIID
-explained mechanism with structural modeling & kinetic experiments
(1/16)
biorxiv.org/content/10.110…
We tested ~7500 53aa tiles covering all 164 yeast transcription factors (TFs) in an activation screen in vivo.
By controlling for fragment expression & sorting GFP activation into 8 bins, we get quantitative measurements with super high signal-to-noise. We found 150 ADs. (2/16)
By controlling for fragment expression & sorting GFP activation into 8 bins, we get quantitative measurements with super high signal-to-noise. We found 150 ADs. (2/16)
Tiles with lots of acidic & hydrophobic residues activated most.
But there are conflicting reports on whether acidic residues are important, so we mutated all the acidic residues in each AD. Besides one interesting case, no mutants activated.
So all yeast ADs are acidic! (3/16)
But there are conflicting reports on whether acidic residues are important, so we mutated all the acidic residues in each AD. Besides one interesting case, no mutants activated.
So all yeast ADs are acidic! (3/16)
