Mass Spec-based #proteomics is such an exciting field. Here is a brief🧵of some great developments focusing on depth/speed
The term proteome appeared in 95/96 science.org/doi/10.1126/sc… https://t.co/6eij4axDzy and (rumors in Australia) coined by Marc Wilkins and co-workers 1/13
Let’s start in 1996 where @labs_mann built on work by Watanabe and co-workers to identify ~150 proteins from yeast 2/13 pnas.org/doi/full/10.10…
Jumping to 2001, @JohnRYatesIII introduced multidimensional protein identification technology (MudPIT) and identified ~1.5K proteins from yeast. 3/13 nature.com/articles/nbt03…
Lots of work in between but a notable mention in 2009, @goodlettlab1 introduced precursor acquisition independent from ion count (PAcIFIC), which built on previous work by Venable and co-workers and others to identify 750 proteins from plasma. 4/13 pubs.acs.org/doi/10.1021/ac…
Also in 2009, @Picotti_Lab with Abersold and co-workers developed targeted assays for a large chunk of the yeast proteome 5/13 nature.com/articles/nmeth…
2013 started to see big gains for mammalian cells with Kelstrup, @thenielsenlab and @jespervolsen identifying ~4K proteins in ~3h. 6/13 pubs.acs.org/doi/10.1021/pr…
2014, the need for speed, @Coon_Labs reported the 1 hour yeast proteome. 7/13 mcponline.org/article/S1535-…
Plasma is a big challenge. Keshishian, Carr and co-workers developed a protocol in 2017 to identify ~4.5K proteins in plasma! 8/13 nature.com/articles/nprot…
Pushing the limits further from their previous work, Kelstrup and @jespervolsen hit almost 6K proteins in 30min. 9/13 pubs.acs.org/doi/10.1021/ac… and also >14K proteins with extended acquisition doi.org/10.1016/j.cels…
Depth is nice but so is robustness! @kusterlab analysed ~2000 samples and hit upto 9K proteins in 16h. 10/13
nature.com/articles/s4146…
>10K proteins in a single run! 2017/2018 work by Reiter and co-workers and @labs_mann 11/13 pubs.rsc.org/en/content/art… nature.com/articles/s4159…
In the last couple of years, things are getting pretty amazing. 5K proteins in 5min from Ralser and co-workers and 6K proteins in 11min from @labs_mann 12/13 nature.com/articles/s4158… biorxiv.org/content/10.110…
I hope you have enjoyed this brief summary. Obviously there are many many others and sorry I can't list them all! Would love to hear what your favorites are below. Thanks for reading to the end 13/13
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