Scientist @unimelb focusing on Proteomics and Metabolism. Loves the contradictions of nature and technology, and the mountains and the ocean.
Jul 29, 2022 • 24 tweets • 23 min read
Mass spec-based #phosphoproteomics has revolutionized the systems-wide analysis of phosphorylation. A 🧵 of some great developments for global analysis of kinase/phosphatase regulation and substrate phosphorylation…1/23
First step is enrichment. PAC, IMAC and MOAC are the most popular. In 1986, Andersson and Porath were one of the first to show that Fe3+ could bind phosphoamino acids sciencedirect.com/science/articl…
In 1997, Ikeguchi & Nakamura first used TiO2 2/23 link.springer.com/article/10.211…
Jul 12, 2022 • 13 tweets • 7 min read
Mass Spec-based #proteomics is such an exciting field. Here is a brief🧵of some great developments focusing on depth/speed
The term proteome appeared in 95/96 science.org/doi/10.1126/sc… https://t.co/6eij4axDzy and (rumors in Australia) coined by Marc Wilkins and co-workers 1/13
Let’s start in 1996 where @labs_mann built on work by Watanabe and co-workers to identify ~150 proteins from yeast 2/13 pnas.org/doi/full/10.10…
