John Beach Profile picture
Technology sales & consulting. Demonstrated insights in medical diagnostics, biochemistry, & use to assess/treat/cure health threats like COVID-19 / SARS-CoV-2

Feb 20, 2023, 6 tweets

@BrownJHM @AaronGoodman33 The Brown Recluse.
As replies note, its bite injects a potent hydrolase enzyme:
Sphingomyelinase D,
a sphingomyelin phosphodiesterase, which breaks sphingomyelin into phosphocholine & ceramide.

Treatment? Wikipedia options sound inadequate.

PubMed inspired many questions!

1/n

@BrownJHM @AaronGoodman33 In PubMed, this caught my eye:
pubmed.ncbi.nlm.nih.gov/25938271/
Sphingomyelinase D/ceramide 1-phosphate in cell survival and inflammation

the venom of Loxosceles, or the toxins of some bacteria such as
Corynebacterium tuberculosis, or
Vibrio damsela
possess high levels of SMase D

2/n

@BrownJHM @AaronGoodman33 As I am not a doctor, I’ll stop here.

I do think, however, that scientists have described the chemistry of Sphingomyelinase D well enough that the bite of a Brown Recluse could be made less necrotic.

Seems like a couple small-molecule solutions would be worth trying.

3/n

“Targeting Loxosceles spider Sphingomyelinase D with small-molecule inhibitors as a potential therapeutic approach for loxoscelism”
pubmed.ncbi.nlm.nih.gov/30734604/

They evaluated benzene sulphonate (a.k.a. benzene sulfonate) compounds.

4/n

Based on other past insights, I searched Google Scholar

cholecalciferol “Sphingomyelinase d”

Plenty to read, & I suspect there are important answers to be found in the relevant ions (Mg, Ca)

5/n

Calcium, Magnesium, proposed relevance of UV exposure & vitamin D3 to activity of the Sphingomyelinase D.

scholar.google.com/scholar?hl=en&…+“Sphingomyelinase+d”&btnG=#d=gs_qabs&t=1676904884988&u=%23p%3D5SjhfhOM7CYJ

In this research, they abolished activity of BRSV SMase D with EDTA

6/n

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