Thread by @_HeartofGrace_

1/ 🚨🧵DNA plasmid contamination in modRNA "vaccines"
DO NOT HAVE TO ENTER THE NUCLEUS
to impact
GENE EXPRESSION, and be implicated in CANCER RISK!
(I said this on April Moss TV MONTHS ago talking about proteomics and gene expression!)
STUDY BELOW in somewhat LAYMAN'S TERMS

2/ Study citation: Gao JJ, Diesl V, Wittmann T, Morrison DC, Ryan JL, Vogel SN, Follettie MT. Bacterial LPS and CpG DNA differentially induce gene expression profiles in mouse macrophages. J Endotoxin Res. 2003;9(4):237-43. doi: 10.1179/096805103225001431. PMID: 12935354.

3/ In the study, the researchers investigated the impact of CpG DNA and LPS on gene expression. They exposed macrophages to CpG DNA and LPS (lipopolysaccharide), which are known immune stimulants. CpG DNA is recognized by Toll-like receptor 9 (TLR9) and can

4/ trigger immune responses. LPS, on the other hand, is a component of the cell wall of certain bacteria and is recognized by Toll-like receptor 4 (TLR4).
In more layman's terms, the researchers wanted to understand how genes in the immune system cells (macrophages) react when

5/they're exposed to CpG DNA and LPS.
They used a "microarray" to see what was happening. A microarray is like super microscope for genes. This allowed them to look at thousands of genes at the same time. It's like looking at a huge puzzle with thousands of pieces all at once.

6/Imagine each gene is a light bulb, and when it's turned on, the gene is doing something. The researchers wanted to see which genes got turned on or off when they added CpG DNA and LPS to the cells.
So, they put the macrophage cells in a dish and added

7/CpG DNA and LPS to the dishes. Then, they used the microarray to see which genes in the cells started to "light up" or "turn off" in response to the CpG and LPS. The researchers got to see how the genes in the cells reacted to CpG DNA and LPS.

8/ It's like the researchers watched how different genes in cells respond to to the CpG DNA motifs (same motif in the DNA PLASMID CONTAMINATION), like watching a light show of genes turning on and off. This helped them understand how CpG DNA and LPS impact cellular genetics.

9/ it is very important to note, the CpG DNA pieces were NOT in the NUCLEUS when gene expression was witnessed! The screenshots are posted above of all the genes they saw impacted by the CpG motif of the DNA and the LPS.
The CpG DNA molecule does not need to enter the cell

10/ nucleus to act on gene expression. CpG DNA engages by interacting with cell surface receptors and in cellular compartments other than the nucleus.
Heavy science: CpG DNA is recognized by Toll-Like Receptor 9 (TLR9), which is located on the cell surface and in endosomes

11/(a cellular compartment). TLR9 specifically recognizes CpG motifs within DNA.
Then CpG DNA binds to TLR9, it triggers a signaling cascade within the cell, leading to the activation of transcription factors like NF-κB and AP-1.
These activated transcription factors then

12/ influence gene expression. They can induce the expression of various genes, including those involved in the immune response, inflammation, and other cellular processes, such as cancer.
The following are genes in the screenshots that are implicated in cancer, and the types:

13/ (not a complete list)
SCYA4 MIP-1b: breast cancer and colorectal cancer.

Vasodilator-stimulated phosphoprotein (VASP): lung, prostate, colorecta, and breast cancer.

Fas antigen (FAS): liver, breast, ovarian, and cervical cancer.

Plasminogen activator inhibitor, type II

14/ (SERPINB2): Implicated in various cancers, including breast, prostate, and colorectal cancer.

Tissue inhibitor of metalloproteinase-1 (TIMP-1): breast, lung, and colorectal cancer.

Plasminogen activator inhibitor, type I (SERPINE1): breast, lung, and ovarian cancer.

15/ CD44 antigen (CD44): breast, pancreatic, head and neck cancers, and other cancers.

Selenoprotein W, muscle 1 (SEPW1): Although not directly associated with cancer, it is involved in antioxidant defense, which may have implications in cancers related to oxidative stress.

16/ Glia maturation factor-b homolog (GMFB): plays a role in neuroinflammatory diseases, which may indirectly relate to some cancers through inflammation-related pathways.

These genes may play roles in various cancer types, either directly contributing to cancer progression or

17/ through mechanisms related to inflammation, oxidative stress, and metastasis.
Also, in some cases, abnormal DNA methylation patterns, including changes in CpG methylation, have been linked to cancer.
These genes are implicated in cancer. Cancer is a VERY COMPLEX PROCESS.

18/ These single genes are most likely not enough alone to directly drive cancer, but they are involved in it. These genes are being impacted OUTSIDE of the nucleus, and do not take into account if the DNA plasmid pieces in the lipid nanoparticle enter the nucleus.

19/ These are some of the genes implicated in the immune system and various DISEASE:
Interleukin 1b (IL-1b): various inflammatory diseases.
SCYA5 Rantes: inflammation
SCYA7 Intercrine: inflammation.
SCYA2 MCP-1: inflammatory diseases.
TNF-a: Tumor necrosis factor-alpha

20/ is associated with inflammation and autoimmune diseases.
CSF3 (G-CSF): Granulocyte-colony stimulating factor, plays a role in regulating white blood cell production.
PBEF: various diseases, including metabolic and inflammatory conditions.

21/ Vasodilator-stimulated phosphoprotein: cardiovascular and vascular diseases.
IL-1 receptor antagonist: inflammatory diseases.
TNFRsf1B: autoimmune diseases
TNFRsf5: autoimmune disease
Adenosine A2b receptor: Linked to cardiovascular and inflammatory disease

22/ 🚨PLAUR: Urokinase plasminogen activator receptor: cancer and tissue remodeling.
Fas antigen: Associated with apoptosis and autoimmune diseases.
Fc-g-R1: autoimmune diseases
Ubiquitin specific protease 18: ubiquitin system, associated with cancer and neurological diseases.

23/ Plasminogen activator inhibitor, type II: blood clotting and fibrinolysis.
Tissue inhibitor of metalloproteinase-1: Regulates tissue remodeling, various diseases, including cancer.
Plasminogen activator inhibitor, type I: Regulates fibrinolysis and blood clotting disorders.

24/ Cathepsin C: immune responses and genetic disorders.
CD44 antigen: cancer and inflammation.
Glia maturation factor-b homolog: neuroinflammatory diseases.
Integrin-associated protein: various diseases.
these genes in disease can be complex and may vary depending on context

25/ and the specific disease in question. These genes are often involved in multiple pathways and processes, making their roles in disease multifaceted.

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26/ The genes impacted by the CpG motif:
Interleukin 1b: Associated with immune response.
SCYA5 Rantes: Involved in immune response.
SCYA7 Intercrine: Associated with immune response.
SCYB2 MIP2: Related to immune response.
Interleukin 1 alpha: Part of the immune response.
SCYA2 MCP-1: Associated with immune response.
TNF-a: Involved in the immune response.
SCYA3 MIP-1a: Part of the immune response.
SCYB10 IP-10: Has a role in the immune response.
SCYA4 MIP-1b: Associated with immune response.
CSF3; G-CSF: Cytokine involved in immune response.
SCYA12 MCP5: Associated with cytoskeletal/extracellular matrix function.
Interleukin 10: Immune-related.
SCYA9 C10-like: Involved in immune response.
Interleukin 18: Part of the immune response.
PBEF: Associated with proliferation/differentiation.
Adenosine A2b receptor: Related to oxidative stress.
PLAUR: Involved in oxidative stress.
Glycoprotein 49 B: Part of the oxidative stress response.
Fas antigen: Related to apoptosis.
Ornithine decarboxylase: Part of the enzyme category.
2¢-5¢ oligoadenylate synthetase 1A: Involved in protein synthesis.
Purine-nucleoside phosphorylase: Enzyme related.
Hyaluronan synthase 1: Related to signal transduction.
TBX1 protein: Part of signal transduction.
DNA segment, Chr 17: Unknown function.

27/ More genes impacted by the CpG motif:
m15131 (Interleukin 1b) - Immune response
u02298 (SCYA5 Rantes) - Immune response
z12297 (SCYA7 Intercrine) - Immune response
X53798 (SCYB2 MIP2) - Immune response
x01450 (Interleukin 1 alpha) - Immune response
m19681 (SCYA2 MCP-1) - Immune response
x02611 (TNF-a) - Immune response
x12531 (SCYA3 MIP-1a) - Immune response
m33266 (SCYB10 IP-10) - Immune response
m35590 (SCYA4 MIP-1b) - Immune response
m13926 (CSF3; G-CSF) - Immune response
u50712 (SCYA12 MCP5) - Cytoskeletal/extracellular matrix
m84340 (Interleukin 10) - Immune response
u19482 (SCYA9 C10-like) - Immune response
D49949 (Interleukin 18) - Immune response
aa691772 (PBEF) - Proliferation/differentiation
X98475 (Vasodilator-stimulated phosphoprotein) - Unknown function
w71236 (WD40 repeat protein 1) - Signal transduction
m74294 (IL-1 receptor antagonist) - Receptor/cell surface
m59378 (TNFRsf1B) - Receptor/cell surface
m83312 (TNFRsf5) - Receptor/cell surface
U05673 (Adenosine A2b receptor) - Receptor/cell surface
ET61664 (Fc-g-RIIB) - Receptor/cell surface
C76481 (PLAUR) - Enzyme
u05265 (Glycoprotein 49 B) - Enzyme
m55637 (HAM-1) - Enzyme
M83649 (Fas antigen) - Apoptosis
M31314 (Fc-g-R1) - Enzyme
u55060 (Lectin, galactose binding, soluble 9) - Enzyme
AA178227 (CD83 antigen) - Protease/inhibitor
x67809 (PPICAP) - Unknown function
m11284 (MHC class I Qa-Tla) - Transport
AA034646 (CC chemokine) - Transport
u59463 (Proprotein convertase subtilisin/kexin type 3 receptor 1-like 2) - Enzyme
M65027 (Glycoprotein 49 A) - Enzyme
aa690738 (ABCA1) - Enzyme
x66081 (CD44 antigen) - Enzyme
AA072961 (Integrin-associated protein) - Enzyme
u16985 (Lymphotoxin B) - Enzyme
aa177433 (Fc epsilon beta) - Transport
u77460 (Complement C3a) - Transport
M73696 (SLC2A1 glucose receptor 1) - Transport
I13732 (SLC20A1 phosphate transporter 1) - Transport
aa691772 (Complement C3a receptor 1) - Transport
I04275 (Scavenger receptor) - Transport
D14883 (CD82 antigen) - Transport
U21795 (Interleukin 2 receptor, gamma chain) - Receptor/cell surface
X54149 (Myeloid differentiation primary response 118) - Proliferation/differentiation
C76739 (Macrophage C-type lectin) - Receptor/cell surface
L10244 (Immediate early response 3) - Signal transduction
m64291 (COX-2) - Enzyme
x03479 (Serum amyloid A 3) - Enzyme
L32973 (Thymidylate kinase TDKI) - Enzyme
m92649 (Mouse nitric oxide synthase) - Enzyme
aa445671 (Glycerol kinase) - Enzyme
U44088 (T-cell death associated gene) - Apoptosis
s64539 (Ornithine decarboxylase) - Enzyme
m33863 (2¢-5¢ oligoadenylate synthetase 1A) - Protein synthesis
x56548 (Purine-nucleoside phosphorylase) - Enzyme
u19118 (Activating transcription factor 3) - Transcription factor
D82964 (Hyaluronan synthase 1) - Signal transduction
m22326-2 (Early growth response 1) - Transcription factor
D17571 (Zinc finger protein 147) - Transcription factor
L10244 (Spermidine/spermine N1-acetyl transferase) - Enzyme
AA163244 (Fructose-6-phosphate 2-kinase) - Transcription factor
Y11666 (Jumonji) - Transcription factor
aa051486 (HMG protein 14) - Transcription factor
aa245216 (Flavo-binding protein) - Unknown function
W44201 (SEC23B homolog) - Signal transduction
U88328 (Cytokine inducible SH2-containing protein 3) - Signal transduction
U19119 (Interferon inducible protein 1 receptor alpha) - Signal transduction
X61940 (Tyrosine phosphatase, non-receptor type 16) - Signal transduction
x81627 (LCN2, 24p3) - Signal transduction
m83218 (S100 calcium binding protein A8) - Signal transduction
U53219 (IFN-g induced GTPase) - Signal transduction
M63630 (IFN-g inducible protein, 47 kDa) - Signal transduction
X17459 (Recombining BP suppressor of hairless) - Signal transduction
U20159 (Lymphocyte cytosolic protein 2) - Signal transduction
aa462486 (Map kinase phosphatase A2) - Signal transduction
aa185007 (Proteasome alpha type 6) - Enzyme
j03023 (Hemopoietic cell kinase) - Signal transduction
U36277 (NF-kB inhibitor) - Signal transduction
m33203 (Heme oxygenase 1) - Enzyme
m65029 (Translation initiation factor 2 alpha kinase 2) - Enzyme
k01496 (Complement factor B) - Enzyme
x16440 (Feline sarcoma viral oncogene homolog) - Enzyme
X80638 (Aplysia ras-related homolog 9) - Enzyme
D87691 (ETF1) - Enzyme
u51907 (NF-kB activator) - Signal transduction
x00246 (Histocompatibility 2, D region locus 1) - Unknown function
AF006040 (Fas death domain-associated protein) - Unknown function
AF019249 (N-mcy (and STAT) interactor) - Unknown function
m57696 (Yamaguchi sarcoma viral oncogene homolog) - Unknown function
AA097231 (RAS-related C3 botulinum substrate 2) - Unknown function
L16462 (B-cell leukemia/lymphoma 2) - Unknown function
X76850 (MAP kinase-activated protein kinase 2 related protein A1a) - Unknown function
u78031 (Bcl2-like) - Unknown function
AA170444 (Ube11 similar to ubiquitin) - Unknown function
x84797 (Hematopoietic cell specific Lyn substrate 1) - Unknown function
AF001863 (FYN binding protein) - Unknown function
aa407010 (KIAA0970) - Unknown function
J03368 (Myxovirus resistance 2) - Unknown function
AF013114 (Epstein-Barr virus induced gene 3) - Unknown function
AF026124 (Phospholipase D3) - Unknown function
W40735 (EH-domain containing 1) - Unknown function
aa184871 (DUS6 dual specificity phosphatase) - Unknown function
aa273574 (E2 ubiquitin conjugating enzyme) - Unknown function
U14103 (Guanine nucleotide dissociation stimulator, -like 1) - Unknown function
U70139 (Carbon catabolite repression 4) - Unknown function
AA060409 (Glia maturation factor-b homolog) - Unknown function
aa673503 (Riken 1300002F13) - Unknown function
m59821 (Immediate early response 2) - Transcription factor
X61800 (C/EBP-d) - Transcription factor
J03776 (Interferon regulatory factor 7) - Transcription factor
m58691 (Zinc finger protein 36) - Transcription factor
U06924 (STAT 1) - Transcription factor
M31885 (Inhibitor of DNA binding 1) - Transcription factor
L20315 (Macrophage expressed gene 1) - Transcription factor
Y08460 (Degenerative spermatocyte homolog) - Transcription factor
L20315 (NF-kB p105) - Transcription factor

@brucep13
(Check out the other threads--especially the ones on zeta potential. Highly negative zeta potential on LNPS cause an impact on biodistribution. Positively charged LNPSs have a tropism for the lungs. neutrial go to liver. Slightly negative go to spleen. Very negative leak into vasculature, and impact vascular (and heart). The DNA plasmid contamination will increase the LNPs to form a DNA/RNA polylipoplex--which will shoot the zeta potential on these things from -3 mV to when they were measured in bulk solvent, which are actually almost -9 in the bloodstream, to farther closer to at least -20 or higher, causing it to bing with the endothelial, causing the RNa to express spike right into the bed of the vesselsas it remodels the endothelium and impacts the permeability, and can lead to hypercoaggulation.

@DJSpeicher Got plasmids?

@TheChiefNerd This is really bad. No genome integration has to occur.

28/ IN MOST CASES, NO GENOMIC INTEGRATION IS REQUIRED. LITIGATION TIME
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