1/ There are some very cool things about the bacteria that are in our guts. We have gram positive, and gram negative bacteria hanging out.
These are not named due to, charge.
Bifidobacteria is a gram positive bacteria. 2/ Gram-positive bacteria have a thicker peptidoglycan layer in their cell wall compared to Gram-negative bacteria.
Gram-positive bacteria lack an outer membrane, which in Gram-negative bacteria contains porins that can facilitate the entry of molecules like lipid nanoparticles.

THE GUT MICROBIOME, and COVID:
The microbiota in your gut is responsible for peripheral cGAS-STING activation, promoting resistance to systemic viral infections like COVID.
If you give an antibiotic during a viral infection, you risk inactivation of cGAS STING to deal with that viral infection, systemically.
Your gut microbiome is not some lone ranger just hanging out.
Drinking, smoking, AND specific antibiotic use, will knock down numbers of bifidobacteria (some antibiotics, even one course will reduce for A YEAR).

THIS is why changes in the gut microbiome are correlated to higher disease outcomes in people with COVID.
The cGAS STING pathway was not fully functioning due to disruptions in the gut.

Now, in the studies done by people who wrote about the gut microbiome and covid, where is your data on your participants who:
DRINK
SMOKE
USE DRUGS
ANTIBIOTIC USE?

THEY never checked. They never accounted for people who drink, smoke, use drugs, or have used antibiotics in the last year.

cell.com/immunity/fullt… 2/ The people who ran the studies on covid and the microbiome, never showed data on those who have used antibiotics, especially in the last year.
Amoxicillin
Doxycycline
Azithromycin

These things impact your cGAS STING pathway, and not in a good way.

Antibiotics are very helpful, WHEN NEEDED.

However, more than one of these reduces bifidobacteria and other species, for up to a year in the gut.

Therefore, you just fecked your cGAS STING,
christiegrace.substack.com/p/the-effects-…

1/🧵 Lockdowns, the last four years, and " Do you get the impression that we're being worn down towards some end?"
Mike Yardley says "Please RT and discuss.".
How one might view this all through the lenses of Joost Meerloo, Gavin de Becker, Psychology, and Neurobiology:

https://twitter.com/YardleyShooting/status/1779239983359897730

2/ Pulling much from the book, "The R@pe of the Mind" by Meerloo: Joost Meerloo, was a psychoanalyst and expert in mass and individual brainwashing.
Joost coined the term "menticide", the death of the mind (effectively, making robots out of people).

1/ 🚨 Thread one of two: PLASMIDS.
CONCERNS!!!
"AGC Biologics...has announced its partnership with BioNTech SE...to further supply Plasmid DNA (pDNA) starting material for the Pfizer-BioNTech COVID-19 vaccine, at AGC’s Heidelberg, Germany facility."

archive.ph/2023.08.21-081… 2/ DNA to RNA to a protein. This is how are body makes proteins. DNA was used, not in the form of the double helix structure, but in the form of a plasmid, to make RNA, which was then put inside of a lipid nanoparticle, and then that is what landed in people,

1/ 💉cGAS STING Pathway Activation by DNA Plasmid Contamination, SPIKE, and LPS in modRNA "vaccines": AIDP, Myocarditis, Stroke, Aortic Dissection, and More: Overview, and Biopsy Methods for Detection.
When DNA integration testing fails to produce results, this pathway, should. 2/ This substack breaks down the pathway in more detail, different ways it can be activated, how these are linked to not just the mRNA "vaccines", but also, how this may be the pathway involved in traditional vaccines, and how to test tissues thoroughly.
christiegrace.substack.com/p/cgas-sting-p…

1/🚨💉🧬"FRONT-LOADED" theoretical CANCER RISK to BABIES EXPOSED to mRNA "VACCINES" in the WOMB: With the discovery of DNA plasmid contamination and other factors in the Lipid Nanoparticle, are babies now at higher risk for developing cancer later in life too? 2/ After Kevin Mckernan tested vials of the mRNA vaccines of both Pfizer and Moderna, Dr. Phillip Buckhaults tested many vials too, and found DNA plasmid contamination in all of the vials he tested.
"The DNA can and likely will integrate into the genomes of transfected cells."

1/ Take a break from the heavy science.
ZETA GOES WILD:
Charged based interactions in nature:
How worms, ticks, and other insects use static electricity to help attach to their hosts (including how they attach, to you!)
swarms of insects also create a charge in the atmosphere 2/ Static electricity passively attracts ticks onto hosts

”Static charges of animals result in strong electric fields when close to vegetation

Ticks are pulled by these electric fields across air gaps of several body lengths"

1/ 🚨💉People have asked what kind of LNP goes to the brain? According to one study, all types--cellular uptake is different (microglia versus neuron, etc).
Others speak to the blood brain barrier being difficult to get through.
However, a CHARGE: HURTS/CHANGES YOUR BRAIN. 2/ Study 1: Surface chemistry governs cellular tropism of nanoparticles in the brain
Song, Eric et al. “Surface chemistry governs cellular tropism of nanoparticles in the brain.” Nature communications vol. 8 15322. 19 May. 2017, doi:10.1038/ncomms15322
ncbi.nlm.nih.gov/pmc/articles/P…

1/ 🚨🚨🚨💉
Lipid Nanoparticles of Specific Size and Charge Can Enter the NUCLEUS Without DNA/NLS or SV40, Interact with Histones, and Cause DNA Mutations

(just highlights, its just too much for X/twitter. please read the substack--it is lengthy with explanations.)

A STUDY:

https://twitter.com/_HeartofGrace_/status/1759348552017264760

2/ (photos may or may not load--X issues for some of uploading photos--refer to Substack if needed):

The study: DOI: 10.1002/smll.200800088
CdTe Nanoparticles Display Tropism to Core Histones and Histone-Rich Cell Organelles


sci-hub.se/10.1002/smll.2…
christiegrace.substack.com/p/lipid-nanopa…

1/ 🚨💉🙏Zeta potential is electrokinetic potential of a particle (LNP). It changes in its environment (pH, ions, viscosity, etc), upon entering the body, and can continue to shift.
Zeta directs where the LNP goes.
Zeta is influenced by the net
charges.
What about tumors? 2/ RNA has a negative charge. DNA plasmids have a negative charge. Positively charged lipids inside an LNP have a positive charge. If you add these up, you will have a net charge. If the LNP broke during storage and the RNA (and DNA leaked out) it would have a positive charge.

1/ 🚨💉🧬MEGA THREAD ON MULTIPLE PATHWAYS AFFECTED BY DNA PLASMID CONTAMINATION that exists as a CpG motif, and potential negative health outcomes, depending on exposure (dose amounts unknown/duration/frequency.
(Much Science--layman's on each explained late, time permitting)

https://twitter.com/_HeartofGrace_/status/1706387728286269883

2/ CpG motifs are short DNA sequences that contain a cytosine followed by a guanine nucleotide, linked by a phosphodiester bond. They are recognized by the immune system. When linearized CpG motifs enter the body, they can interact with MANY pathways.
These are in plasmids.

1/ 🚨💉🧬CpG DNA can INDUCE cyclooxygenase-2 and prostaglandin production, which can have negative health impacts. The previous thread focused solely on prostaglandins. There are only so many tweets which can be placed in a thread.
Plasmid DNA=CpG.
Let's focus on both now.

https://twitter.com/_HeartofGrace_/status/1758335479148060901

2/ Purely health impacts (go back to previous thread for study citations please) of dysregulation of cyclooxygenase-2 and prostaglandin production on the human body.

1/ 🚨💉🧬
C@vid "vaccines"=DNA plasmid contamination. DNA plasmid=CpG motif.
CpG DNA INDUCE cyclooxygenase-2 and prostaglandin.
Inside LNP, this could cause:
inflammatory, autoimmune, endometriosis, cancer, infertility, menstrual irregularities, and miscarriage:
STUDIES: 2/ "CpG DNA induces cyclooxygenase-2 expression and prostaglandin production"
Chen et al.
International Immunology, Volume 13, Issue 8, August 2001, Pages 1013–1020,

doi.org/10.1093/intimm…
academic.oup.com/intimm/article…

1/ 🚨💉DNA plasmid contamination in 💉contains an abundance of CpG motifs. CpG can cause HYPORESPONSIVENESS (immune system SUPPRESION, AFTER activation), potentially leading to:
Infections, Dysregulated Immune Response, Autoimmune Disease, Inflammation, and Cancer:
THE STUDIES: 2/ The initial effect of CpG motifs injected via LNP may cause maturation of dendritic cells (DCs).

With DC maturation induced by CpG motifs, there would be an initial enhancement in antigen processing and presenting antigens to T cells. T cells are part of our immune system.

@SenatorRennick @TGAgovau This is just one way.
It could also enter through the nuclear pore if it enters the cell.
(That is dependent on size) @SenatorRennick @TGAgovau 😬

1/ 🚨💉🧬⚡️⚪️
A modRNA "vaccine" w/ negatively charged lipid nanoparticles (LNP--DNA plasmid contamination) should cause charge-induced hydration asymmetry in the human body.
This could lead to harmful body wide system adverse effects.
(zeta potential goes exponential)
A STUDY 2/ The study:
Liposomes and Lipid Droplets Display a Reversal of Charge-Induced Hydration Asymmetry

Published by THE AMERCIAN CHEMICAL SOCIETY
Pullanchery ET AL, 2023
DOI: 10.1021/acs.nanolett.3c02653

pubs.acs.org/doi/10.1021/ac…

1/ 💉🚨February 8th, 2022 I went on Discernable with Matt Wong. I explain zeta potential, negative charges on clots (and other things LNP). I explained this mechanism. I contacted all podcasters, big and small. I was told I could not go on, because I am not a doctor.

https://twitter.com/Johnincarlisle/status/1755675489534988740

2/ Big and small podcasters, I was told that I did not know what I was talking about, even though I DESIGNED projects all day long with RNA and LNP. I was told I was an idiot. I was told I was wrong. I was told it was PEG all day long and nothing else. Nothing more.

1/ 💉🚨🧬+/-
How Lipid nanoparticles (LNPs) cause CLOTS:
(even when neutral--another thread on that later)

Positively charged LNPs can cause clots via Factor VII Activating Protease (FSAP).

Negatively charged LNPs can cause clots via Factor XII Protease

The mechanisms:

https://twitter.com/_HeartofGrace_/status/1722031142851817706

2/ (this is not to be confused with positively charged liposomes or LNPs causing massive thrombosis in the lungs--the lungs contain mucus, which is full of negatively charged proteins called mucins, and the combo is a highly reactive process)

1/ 🚨💉ISSUES WITH ENDOTOXIN TESTING: LIPID NANOPARTICLES: NOT ACCURATE!
Pfizer stated in their SOP they did use the gold standard test for endotoxin testing--LAL--Limulus Amoebocyte Lysate. The problem is, LAL is inaccurate for measuring endotoxins in the presence of lipids. 2/ There has been much talk about lipid nanoparticles and the presence of endotoxin.
There are levels that are set by the FDA on these levels, and their use in research. pubmed.ncbi.nlm.nih.gov/20575063/

1/🚨💉
Nanoparticle size and surface charge determine effects of PAMAM dendrimers on human platelets in vitro

(clots)

A study:

Dobrovolskaia, et al, 2012

ncbi.nlm.nih.gov/pmc/articles/P…
doi.org/10.1021/mp2004…
2/ Polyamidoamine (PAMAM) dendrimers are a class of hyperbranched, nanoscale macromolecules with unique structural and chemical properties. They are widely studied and utilized in various fields, including drug delivery, imaging, and materials science.

1/ 💉LNP: Lipids and Liposomes: GENE DELIVERY to the PLACENTA: A STUDY.

The placenta, like the heart, runs primarily on lipid metabolism.

A thread on lipids used in "gene delivery", LNP entering the placenta, and what occurs when you disrupt lipid balance in the placenta. 2/ First study: "Liposomes as Gene Delivery Vectors for Human Placental Cells"


doi: 10.3390/molecules23051085. ncbi.nlm.nih.gov/pmc/articles/P…