Excited to present a new preprint on how to model E/I balance using #ipsc derived neuronal models. Great work by @brittmossink and nice collaboration with @SchubiNeuro
biorxiv.org/content/10.110…
biorxiv.org/content/10.110…
We describe a new and easy to implement protocol to efficiently generate SST+ and PV+-GABAergic neurons from human iPSCs.
After 7 weeks in culture these GABAergic neurons effectively exert inhibitory control over glutamatergic neurons – at the single neuron and neuronal network level.
IWe show that Cadherin-13, of which dysfunction is linked to autism and attention-deficit/hyperactivity disorder, is a critical regulator of GABAergic modulation in stem cell derived neuronal networks
