"can you link to evidence that the virus was isolated" is becoming fake moon landing level stupid
Study1: 23JAN2020
biorxiv.org/content/10.110…
🧫bronchoalveolar lavage fluid from RT-PCR-positive patient (ICU-06 in Spike tree=WIV04/2019 virus)
🧫successfully isolated nCoV-2019
124 BetaCoV/Wuhan/WIV04/2019 in Vero and Huh7 cells
🦠cytopathic effects (CPE) seen
biorxiv.org/content/10.110…
🧫bronchoalveolar lavage fluid from RT-PCR-positive patient (ICU-06 in Spike tree=WIV04/2019 virus)
🧫successfully isolated nCoV-2019
124 BetaCoV/Wuhan/WIV04/2019 in Vero and Huh7 cells
🦠cytopathic effects (CPE) seen
🦠culture supernatant (s/n) tested (+) by RT-PCR
🦠rabbit antisera against a bat SARS-related coronavirus (SARSr-CoV) reacted with infected cells
🦠electron microscopy (EM) images of virus particles from infected VeroE6 cells
🦠rabbit antisera against a bat SARS-related coronavirus (SARSr-CoV) reacted with infected cells
🦠electron microscopy (EM) images of virus particles from infected VeroE6 cells
🦠antisera from other infected patients neutralised the isolated virus
Study2: 09MAR2020
mja.com.au/journal/2020/2…
🧫nasopharyngeal swab from RT-PCR positive patient
🧫isolated using Vero/hSLAM cell line
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
mja.com.au/journal/2020/2…
🧫nasopharyngeal swab from RT-PCR positive patient
🧫isolated using Vero/hSLAM cell line
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
🦠whole genome sequencing of the virus from cell culture supernatants was successful (BetaCoV/Australia/VIC/01/2020)
Study3: 19FEB2020
ophrp.org/journal/view.p…
🧫nasopharyngeal and oropharyngeal samples used
🧫isolated using Vero cells
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
ophrp.org/journal/view.p…
🧫nasopharyngeal and oropharyngeal samples used
🧫isolated using Vero cells
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
🦠whole genome sequencing of the virus from cell culture supernatants was successful BetaCoV/Korea/KCDC03/202
Study4: 09JUN2020
medrxiv.org/content/10.110…
🧫swab from upper airways & sputa (n=23)
🧫isolated using Vero clone 118 cells
🦠CPE was seen
🦠rabbit antisera against a SARS-CoV NP reacted with infected cells
medrxiv.org/content/10.110…
🧫swab from upper airways & sputa (n=23)
🧫isolated using Vero clone 118 cells
🦠CPE was seen
🦠rabbit antisera against a SARS-CoV NP reacted with infected cells
Study5: 24APR2020
nejm.org/doi/full/10.10…
🧫(n=17)
🧫isolated using Ver-CCL-81 cells
🦠CPE was seen
🦠RT-PCR showed used to confirm viral growth
nejm.org/doi/full/10.10…
🧫(n=17)
🧫isolated using Ver-CCL-81 cells
🦠CPE was seen
🦠RT-PCR showed used to confirm viral growth
Study6: 18MAY2020
wwwnc.cdc.gov/eid/article/26…
🧫faecal samples (n=2)
🧫isolated using Vero E6 cells
🦠CPE was seen, virions seen by EM of culture
🦠patient antisera reacted in an immunofluorescent assay
wwwnc.cdc.gov/eid/article/26…
🧫faecal samples (n=2)
🧫isolated using Vero E6 cells
🦠CPE was seen, virions seen by EM of culture
🦠patient antisera reacted in an immunofluorescent assay
🦠whole genome sequencing of the virus from cell culture supernatants was successful (NC045512.2)
This is not an exhaustive or in any way complete list of successful culture isolations.
But it CLEARLY shows that infectious, viable, "live" SARS-CoV-2 virus *has* been isolated by different labs & from different people, all over the world.
But it CLEARLY shows that infectious, viable, "live" SARS-CoV-2 virus *has* been isolated by different labs & from different people, all over the world.
There also this protocols.io/view/culture-o…
These come from the @CDCgov public health image library (PHIL)
Ref for those is phil.cdc.gov/default.aspx
