(1/5) AML with NPM1 mutation ⬇️
Characteristically prominent nuclear invaginations imparting a “cup-like” or “fish-mouth” morphology, with very strong MPO positivity 🔬
@KirillLyapichev @sanamloghavi
#hemepath #hemepathMDA #PathTwitter
Characteristically prominent nuclear invaginations imparting a “cup-like” or “fish-mouth” morphology, with very strong MPO positivity 🔬
@KirillLyapichev @sanamloghavi
#hemepath #hemepathMDA #PathTwitter
(2/5) Cytoplasmic staining of NPM1 protein by IHC is predictive of NPM1 mutations
#hemepath #hemepathMDA #PathTwitter
#hemepath #hemepathMDA #PathTwitter
(3/5) This is because NPM1 mutations cause loss of the nucleolar localization signal and addition of a nuclear export signal, leading to increased protein export from
the nucleus and aberrant accumulation in the cytoplasm
#hemepath #hemepathMDA #PathTwitter
the nucleus and aberrant accumulation in the cytoplasm
#hemepath #hemepathMDA #PathTwitter
(4/5) On flow cytometry analysis, AML with mutated NPM1 is characterized by high CD33 expression and variable
(often low) CD13 expression
#hemepath #hemepathMDA #PathTwitter
(often low) CD13 expression
#hemepath #hemepathMDA #PathTwitter
(5/5) In addition, KIT (CD117) and CD123 expression is commonly seen and HLA-DR is
often negative
#hemepath #hemepathMDA #PathTwitter
often negative
#hemepath #hemepathMDA #PathTwitter
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