BION, however, does not appear to be integrated into the ER membrane co-translationally as the protein lacks both a predictable signal sequence and a transmembrane helix such as INM-SM
➡Because it has been demonstrated that disruption of bion has effects on progeny virus production rather than viral genome replication (Kokusho et al., 2016), we examined PR formation in Δbion-infected cells.
The oligomeric state and the existence of intrinsically disordered regions of VP3 might be the key to determining the molecular basis of its ability to generate a variety of functions.
(1) Plasmids expressing a Halo-tagged BION protein (Halo-BION) under the control of the Drosophila hsp70 promoter and the mammalian CMV promoter were constructed by insertion of the PCR product containing the bion open reading frame into the SalI
(2) -Bam...HI sites of pHE-C and pHalo7-C1 respectively. pHalo7-C1 was produced by replacement of the GFP open reading frame in pEGFP-C1 with the HaloTag open reading frame from the pFN19K HaloTag T7 SP6 Flexi Vector (Promega).
What's that I see... Is that a magnetotactic species... 😲
Magnetotactic bacteria is a class of bacteria known to use magnetic fields for orientation. ... Cryptochromes are therefore thought to be essential for the light-dependent ability of the fruit fly Drosophila melanogaster
Hey Bob! I found Bob Turtle too!
Also found our old friend CRYPT O Chrome! 🧬
Sneaky... The old cryptochrome, magnetic field and the formation of Reactive Oxygen Species trick!
Didn't see that coming! 🤣
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