So was Wakefield rightfully scrutinized and Drosten given a hall pass?
Many of the concerns raised about Wakefields qPCR are significant. But the one most cited (contamination) by Dr. Bustin was not.
He claimed Wakefield omitted an RT-Step.
Bustin is emulating this in 2020.
Wakefield is also nailed for Lack of an SOP.
Same story in the Drosten review.
The proper way to address DNA or RNA contamination is with DNases and RNases.
RT enzymes are active at RTemp and as the cycler ramps its temperature up to 95C.
#BustinBusted nature.com/articles/s4159…
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Bustin continues to push for limited to No RT step in qPCR.
Precisely what he persecuted Wakefield for…and was paid handsomely for the testimony he has since contradicted.
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It is well known from BioNtech (Lenk et al) and Sutton et al (1997) that RNA:DNA hybrids inhibit DNaseI.
What is less known is that Quadruplex Gs also inhibit DNaseI. Some exist in SV40 and we get the most signal from this qPCR amplicon.
2 Different mechanisms of DNaseI inhibition = plasmid fragmentation cannot be assumed to be uniform in the mRNA vaccines. Hence 100 fold more spike than parts of the vector.
Below is a map of the codon optimized spike where Quadruplex Gs are overlaid with GAA repeats. GAA's are stickier RNA:DNA hybrids.
Our qPCR primers are overlayed as well.
@RetsefL @KUPERWASSERLAB @weldeiry @JesslovesMJK @DJSpeicher @TracyBethHoeg @DrJBhattacharya
Evans et al demonstrate DNaseI is resistant to quadruplex Gs. They also move to alternative enzymes. nature.com/articles/s4152…
This is from Lenk et al (BioNtech).
Note the concern over GAA sequences and RNA:DNA hybrids.
Our spike qPCR primers happen to land on GAA rich and quadruplex G rich regions of Spike. frontiersin.org/journals/molec…
@LocasaleLab I find the RNAi worship incongruent will how that entire field was ignored when injecting billions of people with mRNAs.
There are multiple 21bp homologies to human that emerged from the haphazard codon optimizations in the modRNA vaccine and no one cared?
@LocasaleLab Receipts.
Take Pfizers vax Sequence and run it through BLAT.
Hello... anyone from the RNAi space want to speak up about this?
On Veterans Day @CharlesRixey @JesslovesMJK
Stopped by MGC.
2 long days later we had all of his data.
It’s now published in The Journal of Independent Medicine.
It describes the mechanism of failure for the DNA contamination in the mRNA shots and why the regulators are missing it.
@JesslovesMJK @CharlesRixey @weldeiry @KUPERWASSERLAB @RetsefL @DrJBhattacharya @RWMaloneMD @RobertKennedyJr @TracyBethHoeg
Another Achs et al Fumble uncovered.
These folks don't even understand Capillary Electrophoresis.
Its becoming increasingly clear these are not honest mistakes but designed to deceive by people who are employed at a Vaccine Research Institute.
Science for Sale.
@JesslovesMJK @DJSpeicher
Here is the Rub.
They used a CE instrument that has a lower limit of sensitivity above the 10ng limit.
You need to be able to pick up 10X below the 10ng limit. Or 33pg/ul (300ul dose @ 10ng = 33pg/ul)
This is embarrassingly rigged or they are incompetent.
They ignored our comments on the preprint server and raced their paper into @Nature.
@JesslovesMJK and @DJSpeicher have their own substacks picking up other errors in this paper I'll post sortly.
The Dead Man switch has been lifted. Im not dead but the paper has been accepted.
More edits may appear as the Journal typesets this but this stack has the most current versions, Nostr links, Bitcoin links and Zenodo links.
Why this matters-
This paper demonstrates why the regulators cant find the DNA. The nucleases being used to remove the DNA, fail to digest the RNA:DNA hybrids. So they remove the KAN gene, fail to remove the Spike gene and only go looking for the KAN gene. This is why fluorometry sees ~100X more than qPCR.
And once again, you dont have to believe us. @CanningPharm showed you a paper from BioNtech that spells this out.
They knew this but pointed the regulators to an assay that wouldnt find it.