Pay attention to IVM/FenBen for cancer.

IVM prevents SV40 promoters from entering the nucleus but blocking importin A/B

https://twitter.com/makismd/status/1890757969622499412

Yes, ivermectin is known to inhibit importin α/β-mediated nuclear transport, which is relevant in the context of SV40 promoters.

Mechanism:

•Importin α/β Pathway: This transport system is responsible for shuttling proteins with nuclear localization signals (NLS) into the nucleus. Many viruses, including SV40, hijack this pathway to deliver their regulatory proteins (e.g., Large T antigen) into the nucleus for replication and transcriptional activation.
•Ivermectin as an Inhibitor: Ivermectin binds to importin α/β and disrupts its function, thereby preventing nuclear entry of proteins that depend on this transport mechanism.

Implications for SV40 Promoters:

•SV40 Promoters: The SV40 early promoter is often used in molecular biology due to its strong transcriptional activity in mammalian cells. However, its transactivation requires the nuclear localization of SV40 Large T antigen, which depends on importin α/β.
•Blocking Nuclear Entry: If ivermectin blocks importin α/β, it could prevent SV40 Large T antigen from entering the nucleus, thereby reducing SV40-driven gene expression and viral replication.

Experimental Evidence:

•Studies have demonstrated ivermectin’s ability to inhibit nuclear import of viral proteins from various RNA and DNA viruses (e.g., HIV-1, Dengue, and even SARS-CoV-2).
•SV40 Large T antigen is known to require importin α/β for nuclear entry. If ivermectin blocks this pathway, it could theoretically interfere with any SV40-driven transcription or replication in systems using this promoter.

Would you like references or more experimental details on this?

I watched this last night as people had questions about the gene therapy described in it.
A thread on my take

https://twitter.com/bryan_johnson/status/1866832888605598099

Background.
Im against the drug war. People should be free to Biohack. Even if the government mandates water, I have a problem with it. Not the water but the force and threat to freedom.
I'm glad Bryan is on this journey and sharing what he finds.

Profiling circulating DNA is a thing. I’m just skeptical of the tox seen with LNP delivery of mRNA.
We took the cover of Science Translational Medicine in 2010 with profiling circulating tumor DNA.

pubmed.ncbi.nlm.nih.gov/20371490/ I’ve used these profiles to characterize my father’s prostate cancer.
BRAF-K601E Mutation called for AKT1 inhibitors.
Some Cannabinoids are AKT1 inhibitors.
David Meiri does some of the best work on profiling cannabinoids for cancer

Holy Sh$t
@P_J_Buckhaults just sent me this.

File under: DOES NOT STAY IN THE DELTOID!

nature.com/articles/s4158…
@P_J_Buckhaults The reason this is a Holy Sh$t is that the resolution and sensitivity is a game changer.
They dont need to load up 100X the dose to track this.

Ivermectin and SV40. pmc.ncbi.nlm.nih.gov/articles/PMC74…

🔥My Review of the Recent FDA-Student DNA contamination Paper.🔥 6-470X the limit but this higher limit is from UV spec which is likely inflating the signal

@blind_nut @DopplerEffect93 @TheStockRizzer @Crash_the_Flash @Double_Christ @MdBreathe Matthew is too incompetent to map reads.
If he could map reads he’d realize Chakraborty doesn’t need peer review because he is using data from 2 peer reviewed papers.
Doah.

anandamide.substack.com/p/chakraborty-… @blind_nut @DopplerEffect93 @TheStockRizzer @Crash_the_Flash @Double_Christ @MdBreathe DNA doesn’t need to get into the nucleus to cause problems.
But it does.

Have a look at this. Globin depletion will suppress the BNT162b2 signal. It has a known Beta Globin 5' UTR

This is a dense read but it will help you to understand why the DNA fluorometer data is lighting up so much on modRNA. This is much more cross-talk than you see with traditional ssRNA.
That is because these are intercalating dyes that bind to minor grooves are only found in double stranded nucleic acids. The fact that RNaseA is showing such a reduction in signal is because the RNA isnt single stranded.
At least half of it is digestable by a dsRNA specific nuclease known as RNaseIII.
Another portion responds to RNaseH which only digests RNA in RNA:DNA hybrids.

This is worth a full read.
They really knocked it out of the park on this study.

publichealthpolicyjournal.com/biontech-rna-b… First…
They measure how long is spike expressed in cells.
7 days out it is higher than day 1. Peaks at day 5.

If there is one paper you should read this weekend…

This is it.

It’s not too thick.

journals.asm.org/doi/pdf/10.112… I’ve posted on this before and have a stack about this if you want a TL/DR

They demonstrate research plasmids used to study the vaccine, infect lab staff and their housemates.

Your response to peoples request for your qPCR protocol is misinformation.
No transparency on your methods but we can already see that you are using only 1 assay in the vector (Kan gene) so you are under estimating the load 100X.

You should know this by now as Speicher et al published this a year ago. @TGAgovau This is misinformation. Dr. David Speicher's latest test used RNaseA. There is no RNA interfering with the fluorometry.
Did you not even read the report?
Life must be good at the TGA when you can be a year behind all the time.

Is SV40 Large Tumor Antigen required for SV40 origin of replication activity.

No Here we detect Pfizer plasmid DNA in a colon tumor biopsy 1 year after vaccination.

And its not small amounts of DNA. Its so much DNA that it can only be explained by plasmid amplification post vaccination or genome integration and amplification.

Just take the 500ng Pfizer sample
If you assume the medium fragment size is 200bp.

How many DNA molecules per dose?

https://twitter.com/dystopian_du/status/1836161446172082469

Green is after RNAseA removes the RNA.
So 500ng for one Pfizer lot and 1500ng for Moderna(right).

That’s high for Moderna. They are usually cleaner.

This is flat out evil.
Imagine whitewashing many deaths of the unborn for a few academic points.

https://twitter.com/retsefl/status/1827585299246899392

Author now has a Kush Job at Pfizer

What a bomb shell.
@CanningPharm sent this to me. Is it a Gene Therapy?
They certainly used plasmids from their Gene Therapy Division.

ncbi.nlm.nih.gov/pmc/articles/P…

The low wattage take that any attention given to GOF steals attention from ‘my pet thesis’, is two logical fallacies in one.
False dichotomy and a zero sum fallacy.
It is possible to be concerned about both GOF and lockdown tyranny at the same time.
Like chewing gum and walking.

https://twitter.com/jengleruk/status/1797922972067618863

There are usually 2 reasons such nonsense gets blathered about.

1)someone doesn’t want you to look at GOF.

2)marketing of substacks requires one differentiate from what’s currently capturing attention and appear divergent.

The latest scoobie snack is that RNA viruses mutate too quickly to ever spread… therefore GOF is all kabuki theatre.

This is clearly refuted by the sequencing data but let’s assume the argument stands…
Are these folks unaware of synthetic genomic projects making DNA viruses? Epstein-Barr is a dsDNA herpes virus in 90% of the population. Clearly it can spread and it’s only 172kb.
Well under the size of the mycoplasma genome synthesized in 2008.

jcvi.org/research/synth…

Posting again to remind folks that spike protein can drive mitophagy.

Now that we know the modRNAs are prone to frameshifting and there is a Mito peptide after the Pfizer stop codons, it wouldn’t surprise me if vaxxed patients have lower extracellular Mito.

ncbi.nlm.nih.gov/pmc/articles/P… A chimeric spike-Mito peptide would turn the immune system against extracellular Mito.

sciencedirect.com/science/articl…

Alphafold3 on P53 and the Spike sequence.
One region of confident interaction.
golgi.sandbox.google.com/fold/3effaf176…
Input sequences
Its limited to 5000 bases as input so this is just the spike sequence from BNT162b2 Pbiv.

Debunk the False dichotomy.

If the vax doesn’t prevent infection then how does injecting more spike address a spike driven p53 suppression?

This vax vs C19 is tiresome given the vaxx doesn’t prevent C19 infection.

https://twitter.com/debunk_the_funk/status/1787653574932975676

There 1-100B single copy virions in peak infection.

Asymptomatic infection is likely lower than this.

14Trillion to 42Trillion modRNAs injected coding for spike in a failed attempt to prevent you from getting a spike infection.

pnas.org/doi/full/10.10…