If there is one paper you should read this weekend…

This is it.

It’s not too thick.

journals.asm.org/doi/pdf/10.112… I’ve posted on this before and have a stack about this if you want a TL/DR

They demonstrate research plasmids used to study the vaccine, infect lab staff and their housemates.

Your response to peoples request for your qPCR protocol is misinformation.
No transparency on your methods but we can already see that you are using only 1 assay in the vector (Kan gene) so you are under estimating the load 100X.

You should know this by now as Speicher et al published this a year ago. @TGAgovau This is misinformation. Dr. David Speicher's latest test used RNaseA. There is no RNA interfering with the fluorometry.
Did you not even read the report?
Life must be good at the TGA when you can be a year behind all the time.

Is SV40 Large Tumor Antigen required for SV40 origin of replication activity.

No Here we detect Pfizer plasmid DNA in a colon tumor biopsy 1 year after vaccination.

And its not small amounts of DNA. Its so much DNA that it can only be explained by plasmid amplification post vaccination or genome integration and amplification.

Just take the 500ng Pfizer sample
If you assume the medium fragment size is 200bp.

How many DNA molecules per dose?

https://twitter.com/dystopian_du/status/1836161446172082469

Green is after RNAseA removes the RNA.
So 500ng for one Pfizer lot and 1500ng for Moderna(right).

That’s high for Moderna. They are usually cleaner.

This is flat out evil.
Imagine whitewashing many deaths of the unborn for a few academic points.

https://twitter.com/retsefl/status/1827585299246899392

Author now has a Kush Job at Pfizer

What a bomb shell.
@CanningPharm sent this to me. Is it a Gene Therapy?
They certainly used plasmids from their Gene Therapy Division.

ncbi.nlm.nih.gov/pmc/articles/P…

The low wattage take that any attention given to GOF steals attention from ‘my pet thesis’, is two logical fallacies in one.
False dichotomy and a zero sum fallacy.
It is possible to be concerned about both GOF and lockdown tyranny at the same time.
Like chewing gum and walking.

https://twitter.com/jengleruk/status/1797922972067618863

There are usually 2 reasons such nonsense gets blathered about.

1)someone doesn’t want you to look at GOF.

2)marketing of substacks requires one differentiate from what’s currently capturing attention and appear divergent.

The latest scoobie snack is that RNA viruses mutate too quickly to ever spread… therefore GOF is all kabuki theatre.

This is clearly refuted by the sequencing data but let’s assume the argument stands…
Are these folks unaware of synthetic genomic projects making DNA viruses? Epstein-Barr is a dsDNA herpes virus in 90% of the population. Clearly it can spread and it’s only 172kb.
Well under the size of the mycoplasma genome synthesized in 2008.

jcvi.org/research/synth…

Posting again to remind folks that spike protein can drive mitophagy.

Now that we know the modRNAs are prone to frameshifting and there is a Mito peptide after the Pfizer stop codons, it wouldn’t surprise me if vaxxed patients have lower extracellular Mito.

ncbi.nlm.nih.gov/pmc/articles/P… A chimeric spike-Mito peptide would turn the immune system against extracellular Mito.

sciencedirect.com/science/articl…

Alphafold3 on P53 and the Spike sequence.
One region of confident interaction.
golgi.sandbox.google.com/fold/3effaf176…
Input sequences
Its limited to 5000 bases as input so this is just the spike sequence from BNT162b2 Pbiv.

Debunk the False dichotomy.

If the vax doesn’t prevent infection then how does injecting more spike address a spike driven p53 suppression?

This vax vs C19 is tiresome given the vaxx doesn’t prevent C19 infection.

https://twitter.com/debunk_the_funk/status/1787653574932975676

There 1-100B single copy virions in peak infection.

Asymptomatic infection is likely lower than this.

14Trillion to 42Trillion modRNAs injected coding for spike in a failed attempt to prevent you from getting a spike infection.

pnas.org/doi/full/10.10…

The paper from @weldeiry

showing spikes interplay with P53 translation and DNA damage and repair pathway sails through peer review in 2 weeks.

oncotarget.com/article/28582/… While much attention has been focused on the nuclear localization, even cytoplasmic DNA can trigger mayhem in cell circuitry. Chronic activation of cGAS-STING can lead to tumorogenesis.

frontiersin.org/journals/immun…

Let play a game.
Can anyone make sense of these contradicting Pfizer-Regulator documents.

So the SV40 Promoter is not responsible for plasmid manufacturing.
But it’s the promoter for the Kanamycin resistance gene? The documents submitted to the EMA show they use 50ug/ml of Kanamycin to replicate the plasmid.
How does that work?
No Promoter, no Kanamycin resistance..
No plasmid manufacturing?

The targeted enrichment of BNT162b2 is working.

We have a 22,000 fold enrichment for plasmid containing sequences from cell lines treated with vaccine. The variants in the plasmid have reproduced themselves for a 3rd time.

We do not see these in the vaccine alone. Only when the vaccine is in contact with OVCAR3 cell lines.

This implies the DNA is active in those cell lines and likely replicating.

Well, well, well,

As health agencies assure the public that the DNA contamination is of no consequence, behind the scenes they are scurrying to have it removed from future vaccines!
No prior vaccine in Canada has been approved with such a sequence contaminant.
@FLSurgeonGen Pfizer assured them the sequence is not material to plasmid manufacturing.
This is an overt lie.
You cannot make plasmids without the promoter for the antibiotic resistance gene.

It is active in mammalian cells.
If it’s not needed, why is it in there?

Let’s dissect Berensons horrible capacity to critically read scientific literature.
“The Best Study Yet”

Says the guy selling a reefer madness book.

Did Alex really read the study or just parrot one that supports his fear porn over a plant?
Lets look

https://twitter.com/alexberenson/status/1781784272740786582

First order of business.
How do these authors make money?

Oh… by treating cannabis use disorder!

You need to associate cannabis with harms in order for society to believe they should pay for treatment.

The authors declare no conflict despite how blatant and overt this is.

Who wants to teach a Yale PhD about reverse transcriptase and what happens to the nucleus during cell division?

For extra credit you can teach her about frameshifting, template switching and dsDNA contamination.

youcanknowthings.com/2020/12/10/wha… While a helpful high school version of biology,
Her statement about the 100% unidirectional nature of DNA was put to bed decades ago when we sequenced the human genome and found 8% of it coded for HERVs.

In true form, the journalist never links to the paper.

Did you not realize that?

If you go digging for it, you’ll see it’s a non peer reviewed study designed to never find a 1:5000 event like myocarditis.
But one person died 4 days after vax and they chalked it up as coincidence.

cdc.gov/mmwr/volumes/7… @Doctor_Eric_B Note, this is a CDC MMWR which is non peer reviewed propaganda.

They have to gall to claim they have no conflicts despite working for the CDC that sells $ billions of vaccines every year.

.@USMortality is sending letters to my employer trying to claim I doxxed him but spelled his name wrong?

That got a chuckle or two.

But at least we know he’s the cancel culture type that got @MartinKulldorff fired.

Stay away from this fraud. I’m going to guess the reason he/it doesn’t want his name correctly spelled as it might risk his employment if people figured out who Ben M is.

But he’s just fine sending in complaints to other people’s employers.

Funny how that works.

There are some interesting comments in this piece.

https://twitter.com/dystopian_du/status/1770950841530515845

Before I address the genetic fallacy..

Doah
217 Jabs....Oh my....Go get your 10th from the CDC No sign of BioNtech Spike sequence in their data.
tinyurl.com/w46vuu4y