Final version of our Ancient microRNA paper out with @RNAJournal out today. We are very happy to see our manuscript online and hope you all enjoy it as we do :-).
A thread on RNA from 14 000 year old samples 1/6
#Paleotranscriptomics rnajournal.cshlp.org/content/27/3/3…
When reading the great study by Smith et al on the #Tumatpuppy ancient RNA sequencing that used a smallRNA sequencing protocol, we immediately thought to dig deeper in their data for #microRNAs. 2/6 journals.plos.org/plosbiology/ar…
Teaming up with them and @CpgSthlm (@love_dalen), we found microRNAs that showed abundances and nucleotide damage patterns (!) comparable to what had been described from historic, but never before from such ancient, >14 000yrs old RNA samples 3/6 Image
Using @MirGeneDB we found more than 300 expressed microRNAs in the data. among those were several canid-specific microRNAs, clearly supporting the taxonomic origin (ancient dog-puppy and historic wolf skins) of the samples. 4/6
Using the modern dog microRNA tissue-atlas we find that the 3 ancient and 2 historic samples cluster very well according to their tissue-origin!! All this after being 14 000 yrs in the permafrost - mindblowing for us! 5/6 Image
This opens up for new paths in the #paleotranscriptomics field with a framework for aRNA analysis developed by us at @FriedlanderLab 6/6

Please RT if you like

• • •

Missing some Tweet in this thread? You can try to force a refresh
 

Keep Current with Bastian Fromm

Bastian Fromm Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!

Follow Us on Twitter!