When reading the great study by Smith et al on the #Tumatpuppy ancient RNA sequencing that used a smallRNA sequencing protocol, we immediately thought to dig deeper in their data for #microRNAs. 2/6 journals.plos.org/plosbiology/ar…
Teaming up with them and @CpgSthlm (@love_dalen), we found microRNAs that showed abundances and nucleotide damage patterns (!) comparable to what had been described from historic, but never before from such ancient, >14 000yrs old RNA samples 3/6
Using @MirGeneDB we found more than 300 expressed microRNAs in the data. among those were several canid-specific microRNAs, clearly supporting the taxonomic origin (ancient dog-puppy and historic wolf skins) of the samples. 4/6
Using the modern dog microRNA tissue-atlas we find that the 3 ancient and 2 historic samples cluster very well according to their tissue-origin!! All this after being 14 000 yrs in the permafrost - mindblowing for us! 5/6