2. Wuhan University ABSL3
Experimental work using humanized mice will be conducted at the Center for Animal Experiment Biosafety 3 lab of Wuhan University at the School of Medicine in Wuhan, China.
3. Humanized mice for experimental infection
"To understand whether bat covs that utilize receptors found in people have potential to infect people, we will use Swiss albino mice (standard breed at Wuhan University) that have been genetically modified to have human receptors"
4. Wuhan University Swiss Albino Mice (2)
"We'll infect them with cultured bat coronaviruses and determine which organs become infected and whether these mice are capable of shedding infectious virus."
5. Humanised Mice with ACE2 or DPP4 genes
"Humanized mice will be genetically modified to carry human ACE2 or DPP4 gene will be used to evaluate pathogenesis of CoVs. We cannot anticipate exactly how many viruses we will find that are candidates for experimental models"
6. 20 Viruses, 80 mice, what could go wrong?
"we estimate that we will use 4 adult mice (2 male, 2 female) per virus and that we will identify approximately 20 viruses that will be used
for mouse infection experiments. This will require a total of 80 mice over the study period"
7. Project Performance Sites
EHA & WIV and WHU
8. Many Biosafety Issues Identified at Wuhan University ABSL3 in our third report (next tweet)
Experimental work using humanized mice will be conducted at:
Center for Animal Experiment BSL 3 lab of Wuhan University
UNC at Chapel Hill
Institute for Pathogen Biology (Beijing)
13. We are sure the mice did shed infectious virus!
"We'll use Swiss albino mice genetically modified with human receptors. We'll infect them with cultured bat coronaviruses to determine which organs become infected & whether these mice are capable of shedding infectious virus"
14. Ever Increasing Numbers of Humanised Mice
Humanized mice genetically modified to carry human ACE2 gene used to evaluate pathogenesis of CoVs
5-6 bat SARSr-CoV strains used for mouse infection experiments
15-20 adult mice per virus strain will require max of 120 mice
15. The biggest joke of all - Lab biosafety
WIV & WHU ABSL3
All experimental work using infectious material will be under appropriate biosafety standards. Disposal of hazardous materials will be conducted according to institutional biosafety regulations.
16. The Second biggest joke - Correct PPE protocols
They lied
We have the photographic evidence and written confessions from Chmura and Zhengli Shi
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material. 2. Replication process can lead to presence of viral DNA & cellular components in culture medium. 3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination
4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System
1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!
The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!
Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!
And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception
Let us expose this falsehood, this house of deceit
& the duties of the vigilant in these perilous times!
Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?
@janeqiuchina
She continued:
"I was equally shocked by Daszak’s constant self-promotion and how effective it was."
@janeqiuchina About the film:
"Blame: Bats, Politics & a Planet Out of Balance"
"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda"