2. Wuhan University ABSL3
Experimental work using humanized mice will be conducted at the Center for Animal Experiment Biosafety 3 lab of Wuhan University at the School of Medicine in Wuhan, China.
3. Humanized mice for experimental infection
"To understand whether bat covs that utilize receptors found in people have potential to infect people, we will use Swiss albino mice (standard breed at Wuhan University) that have been genetically modified to have human receptors"
4. Wuhan University Swiss Albino Mice (2)
"We'll infect them with cultured bat coronaviruses and determine which organs become infected and whether these mice are capable of shedding infectious virus."
5. Humanised Mice with ACE2 or DPP4 genes
"Humanized mice will be genetically modified to carry human ACE2 or DPP4 gene will be used to evaluate pathogenesis of CoVs. We cannot anticipate exactly how many viruses we will find that are candidates for experimental models"
6. 20 Viruses, 80 mice, what could go wrong?
"we estimate that we will use 4 adult mice (2 male, 2 female) per virus and that we will identify approximately 20 viruses that will be used
for mouse infection experiments. This will require a total of 80 mice over the study period"
7. Project Performance Sites
EHA & WIV and WHU
8. Many Biosafety Issues Identified at Wuhan University ABSL3 in our third report (next tweet)
Experimental work using humanized mice will be conducted at:
Center for Animal Experiment BSL 3 lab of Wuhan University
UNC at Chapel Hill
Institute for Pathogen Biology (Beijing)
13. We are sure the mice did shed infectious virus!
"We'll use Swiss albino mice genetically modified with human receptors. We'll infect them with cultured bat coronaviruses to determine which organs become infected & whether these mice are capable of shedding infectious virus"
14. Ever Increasing Numbers of Humanised Mice
Humanized mice genetically modified to carry human ACE2 gene used to evaluate pathogenesis of CoVs
5-6 bat SARSr-CoV strains used for mouse infection experiments
15-20 adult mice per virus strain will require max of 120 mice
15. The biggest joke of all - Lab biosafety
WIV & WHU ABSL3
All experimental work using infectious material will be under appropriate biosafety standards. Disposal of hazardous materials will be conducted according to institutional biosafety regulations.
16. The Second biggest joke - Correct PPE protocols
They lied
We have the photographic evidence and written confessions from Chmura and Zhengli Shi
"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?
@janeqiuchina
She continued:
"I was equally shocked by Daszak’s constant self-promotion and how effective it was."
@janeqiuchina About the film:
"Blame: Bats, Politics & a Planet Out of Balance"
"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda"
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.
Time to spend some of this tax money on local electronics, biological, ecological, IT, optical, chemistry, engineering & physics labs run by local amateurs for the benefit of our communities?
Short 🧵on Primer & PCR Test issues (Pangolin Covs)
1. The key issue with pangolins and Pcovs is that:
It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless
The samples are retested with bespoke primers & more accurate PCR tests available now,
We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.
However, "they" (I will name who they are later) should, and perhaps in the future, may retest the stored samples at WIV, IPB, SCAU AND GIABR, to clarify the question of which coronaviruses were present in their pangolin samples.
2. May have been for research and commercial purposes, but they were caught bang to rights.
"LIU initially stated that he did not know what the materials were and that someone must have put them into his bag"
3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum