2. Wuhan University ABSL3
Experimental work using humanized mice will be conducted at the Center for Animal Experiment Biosafety 3 lab of Wuhan University at the School of Medicine in Wuhan, China.
3. Humanized mice for experimental infection
"To understand whether bat covs that utilize receptors found in people have potential to infect people, we will use Swiss albino mice (standard breed at Wuhan University) that have been genetically modified to have human receptors"
4. Wuhan University Swiss Albino Mice (2)
"We'll infect them with cultured bat coronaviruses and determine which organs become infected and whether these mice are capable of shedding infectious virus."
5. Humanised Mice with ACE2 or DPP4 genes
"Humanized mice will be genetically modified to carry human ACE2 or DPP4 gene will be used to evaluate pathogenesis of CoVs. We cannot anticipate exactly how many viruses we will find that are candidates for experimental models"
6. 20 Viruses, 80 mice, what could go wrong?
"we estimate that we will use 4 adult mice (2 male, 2 female) per virus and that we will identify approximately 20 viruses that will be used
for mouse infection experiments. This will require a total of 80 mice over the study period"
7. Project Performance Sites
EHA & WIV and WHU
8. Many Biosafety Issues Identified at Wuhan University ABSL3 in our third report (next tweet)
Experimental work using humanized mice will be conducted at:
Center for Animal Experiment BSL 3 lab of Wuhan University
UNC at Chapel Hill
Institute for Pathogen Biology (Beijing)
13. We are sure the mice did shed infectious virus!
"We'll use Swiss albino mice genetically modified with human receptors. We'll infect them with cultured bat coronaviruses to determine which organs become infected & whether these mice are capable of shedding infectious virus"
14. Ever Increasing Numbers of Humanised Mice
Humanized mice genetically modified to carry human ACE2 gene used to evaluate pathogenesis of CoVs
5-6 bat SARSr-CoV strains used for mouse infection experiments
15-20 adult mice per virus strain will require max of 120 mice
15. The biggest joke of all - Lab biosafety
WIV & WHU ABSL3
All experimental work using infectious material will be under appropriate biosafety standards. Disposal of hazardous materials will be conducted according to institutional biosafety regulations.
16. The Second biggest joke - Correct PPE protocols
They lied
We have the photographic evidence and written confessions from Chmura and Zhengli Shi
"Assessments were deliberately manufactured & used to discredit some of our nation’s bravest & impede access to medical care. As was the case with other high-visibility intel assessments (Covid-19?), it fell far short of analytic integrity standards"
3. "Today’s action by @TulsiGabbard is a glimmer of hope for our nation’s intelligence officers, service members, & diplomats who have defended this country in austere locations and subsequently had the nation they served turn its back on them"
1. Based on the Parsi calendar conversion and the text of the Persian Mar-Nāmeh (THE BOOK FOR TAKING OMENS FROM SNAKES), seeing a snake today means you should not look at it, otherwise you will soon have a cause to be sorry.
2. Rule 7 cruelly says:
"If you see a snake on the day of Merdad (Amerdid) do not look at it, (or otherwise) you will soon have a cause to be sorry"
According to the Parsees, today is Roj Amardad (also spelled Merdad or Amerdāt).
3. If you see it, how can you not look at it?
The Book for Taking Omens from Snakes (30 Nov 1892)
By President-Surgeon-Major G. Waters.
"The custom of taking omens from snakes seems to be
very ancient. The people of antiquity associated with the snake various peculiar ideas"
Across the centuries & the tracks,
Where bone & muscle give their all,
To answer to the master's call.
And in the pocket, pouch, or hand,
A secret comfort rules the land.
A cheap escape from sweat & pain,
To dull the mind or spike the brain.
And laborers crowd through palace doors,
To drown the shift in burning gin.
While in the slums, for just a penny,
The laudanum drops are shared by many,
To soothe the cough, the cramp, the tear,
And quiet the children crying there.
3. Across the sea, the jungle steams
Where coolies chased their broken dreams.
The shovel bites, the fever burns,
Until the evening sun returns.
Then in the bunkhouse, dark & small,
Sweet smoke rises on the wall;
The opium pipe is lit & blessed,
A pain-free haze, a perfect rest!
@YouTube @Vimeo @rumblevideo @waybackmachine @googledrive @Gemini @grok @CellPressNews @JAMA_current @Nature @NEJM @PLOS @embojournal @VirologyJ @biorxivpreprint @medrxivpreprint 3. One big one I forgot...
@X
That made a vast difference, helping us coordinate our work through chat groups and share our work and keep the public and journalists and U.S. government organizations and commissions informed of the latest updates.
Did ye sing, John Ball,
as they pulled you apart?
A poet speaking for us all
from England’s tattered heart.
What a price, John Ball,
for singing to us all.
When hope came as a crime
in simple rhythm and rhyme.
2. He who sings a blackbird’s song
to a starved & battered throng
with a message to stand strong
would never last long.
So we’ll sing to John Ball
for the helpless & hopeless,
the voiceless, forgotten,
buried beneath the mansions
of Lords; misbegotten
mortar, stones & chandeliers
3. the scarlet grave goods
of England’s preening peers.
We’ll sing in simple rhymes
and hope for better times,
when those barons’ grandchildren,
so viscous, reptilian,
are thrown from their seat
in ignominious defeat.