2. Wuhan University ABSL3
Experimental work using humanized mice will be conducted at the Center for Animal Experiment Biosafety 3 lab of Wuhan University at the School of Medicine in Wuhan, China.
3. Humanized mice for experimental infection
"To understand whether bat covs that utilize receptors found in people have potential to infect people, we will use Swiss albino mice (standard breed at Wuhan University) that have been genetically modified to have human receptors"
4. Wuhan University Swiss Albino Mice (2)
"We'll infect them with cultured bat coronaviruses and determine which organs become infected and whether these mice are capable of shedding infectious virus."
5. Humanised Mice with ACE2 or DPP4 genes
"Humanized mice will be genetically modified to carry human ACE2 or DPP4 gene will be used to evaluate pathogenesis of CoVs. We cannot anticipate exactly how many viruses we will find that are candidates for experimental models"
6. 20 Viruses, 80 mice, what could go wrong?
"we estimate that we will use 4 adult mice (2 male, 2 female) per virus and that we will identify approximately 20 viruses that will be used
for mouse infection experiments. This will require a total of 80 mice over the study period"
7. Project Performance Sites
EHA & WIV and WHU
8. Many Biosafety Issues Identified at Wuhan University ABSL3 in our third report (next tweet)
Experimental work using humanized mice will be conducted at:
Center for Animal Experiment BSL 3 lab of Wuhan University
UNC at Chapel Hill
Institute for Pathogen Biology (Beijing)
13. We are sure the mice did shed infectious virus!
"We'll use Swiss albino mice genetically modified with human receptors. We'll infect them with cultured bat coronaviruses to determine which organs become infected & whether these mice are capable of shedding infectious virus"
14. Ever Increasing Numbers of Humanised Mice
Humanized mice genetically modified to carry human ACE2 gene used to evaluate pathogenesis of CoVs
5-6 bat SARSr-CoV strains used for mouse infection experiments
15-20 adult mice per virus strain will require max of 120 mice
15. The biggest joke of all - Lab biosafety
WIV & WHU ABSL3
All experimental work using infectious material will be under appropriate biosafety standards. Disposal of hazardous materials will be conducted according to institutional biosafety regulations.
16. The Second biggest joke - Correct PPE protocols
They lied
We have the photographic evidence and written confessions from Chmura and Zhengli Shi
"SPIKED tells the inside story of one of humanity’s biggest & most pressing mysteries: the debate over the origins of COVID-19"
Clip 👇
2. The Making of the Documentary (20 minutes)
"Unfolding like true crime, this detective story takes us deep into clashing conspiracy theories & partisanship while exposing the dangers posed by experiments regularly taking place in the world’s biolabs"
"It’s about the future of biotech and the next pandemic. We wanted to make a contentious story really exciting, with twists and turns, that covers the science but with a strong dramatic structure"