Biotech2k Profile picture
Sep 26, 2021 18 tweets 5 min read Read on X
#Viral_Capsids

A look at viral capsids.
1/ The capsid is a strong protein structure that encloses and protects the viral genome. The most basic viruses will use a single protein produced many times to build the capsid. The more complex viruses will use multiple proteins to build their capsid structure.
2/ The basic structure of the viral capsid comes in 3 basic designs. They are Icosahedral, Helical and Complex.
3/ The Helical symmetry of capsids takes 1 protein and links them together into a very long string. That string is then wrapped into a helical structure like a tube.
4/ An example of a helical capsid virus is Ebola. This virus actually looks like a worm because of its helical capsid.
5/ The Icosahedral capsid is made up of triangles to form a sphere like shape. If you ever seen a 20 sided die from the D&D game, that is a perfect example of an icosahedral shape.
6/ The basic unit of the icosahedral capsid is a single viral protein often designated as Viral Protein 1 (VP1). Some viruses will use multiple proteins to form its basic triangle structure and will designate them VP1, VP2 and VP3.
7/ This small structure is the most basic building block of the icosahedral structure. When 3 of these proteins come together, they form the basic triangle of the icosahedral capsid. The single triangle is called a facet.
8/ The most basic virus is made up of only 20 facets. This is also called its T number. A T-1 virus would have 20 facets. A T-2 virus would have 40 facets and a T-3 virus would have 60 facets.
9/ Since each facet is made up of the 3 basic proteins, the T number that represents the facets gets multiplied by 3 to find out the total proteins in the capsid. For example a T-1 capsid has 20 facets with 3 proteins per facet = 60 total proteins.
10/ A T-3 capsid would have 20 x 3 facets or 60 total facets with 3 proteins to make up every facet. That would be 60 x 3 = 180 total proteins.
11/ When a capsid falls into icosahedral symmetry, it will have specific shapes if forms. There are 2 fold, 3 fold and 5 fold axes in each 20 facet capsid.
12/ As the virus capsid gets bigger into the T scale, the number of 5 fold axes increase so that the capsid goes from a basic of triangle faces to pentagon shaped faces made up of these 5 fold triangles shown below.
13/ In a large enough capsid, you will begin to see 6 fold axes of symmetry. They begin to look like the pattern on a soccer ball.
14/ The last Capsid is the Complex which is made up of both helical and icosahedral parts. The classic example of this type of virus is the bacteriophage.
15/ The head of the bacteriophage is made up of a basic icosahedral shape which is attached to a long tube that is made up of a helical structure. These do not harm humans and prey on bacteria hence their names.
16/ The Adeno Associated Virus (AAV) is a basic T-1 icosahedral capsid. Its made up of 20 facets. The most basic uses only 1 protein VP1 with 60 copies to make up the capsid. Others will use 3 different proteins with VP1, VP2 and VP3 in 20 copies of each to build the capsid.
17/ The AAV capsid is the smallest you can find. It only holds about 4,500 bases (4.5kb) of RNA or DNA genetic material.

• • •

Missing some Tweet in this thread? You can try to force a refresh
 

Keep Current with Biotech2k

Biotech2k Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

More from @Biotech2k1

Jun 19
🧵Innovation: (Part 3)

I know in a market that is super bubbly with the indexes trading at over 27x earnings many investors are wondering where to put their money. I know I have been looking and searching for value of any kind in this market.
1/ #Tech is a very big space, but there are some key themes here I am focused on as I think they are still innovative and disruptive. They are #Cloud, #Cybersecturity and #Automation.
2/ Automation is the easiest to cover first as it is all about replacing laborers in repetitive tasks with robots and equipping those robots with controller enabled with #AI and #ML to allow them to learn and improve the processes.
Read 13 tweets
Jun 19
🧵Innovation: (Part 2)

I know in a market that is super bubbly with the indexes trading at over 27x earnings many investors are wondering where to put their money. I know I have been looking and searching for value of any kind in this market.
1/ The next major theme is Financial Technology called #FinTech. This has been very disruptive to the old school financial industry which has been very complicated and unfriendly to consumers for many decades. I know last year I abandoned my obsolete old bank for fintech.
2/ Many of these companies disrupted the space by just building simple to use and very easy apps. I have been doing online banking and trading for over 20 years and its never been easier for consumers. These apps are very simple and very easy to use.
Read 21 tweets
Jun 19
🧵Innovation: (Part 1)

I know in a market that is super bubbly with the indexes trading at over 27x earnings many investors are wondering where to put their money. I know I have been looking and searching for value of any kind in this market.
1/ I have been an innovation investor for many years. I put out a thread a few years ago about my thematic investing style. Some of my top themes at the time where #AI, #Automation, #Cybersecurity in Tech, #Fintech, #TechBio and #CRISPR in Biotech.
2/ Today, were are going to look at these themes again. There were 2 themes I opted out of over that time since my last big thematic post. They were #eVTOL and #SynBio. Both seem to be way too early and way too risky at this stage of development.
Read 21 tweets
Apr 27
🧵Looking at $RXRX Platform:

Here I am going to look at the platform for @RecursionPharma and attempt to value this company as a sum of the parts.
1/ The Recursion platform comes in the technology pieces. They are the Data Collection, Mapping and Machine Learning. I will look at each of these parts. Image
2/ The first part of the platform is Data Collection. This is their automated lab where they run 2 million experiments per week and record that experimental data into their super computer Biohive. Image
Read 14 tweets
Apr 18
The Biotech Report:

This covers all my companies since my last report in March. I will cover my 3 new companies here too.
Read 15 tweets
Apr 15
🧵The Little Book of Biotech: Part 5

This is going to be for companies not inside the $XBI. I will post companies here as I come across biotech names and check them out.
$ABSI is a company using synthetic biology to create antibodies and AI to model and design antibodies. They are working on generative AI platform to allow for antibody development. Its a massive bubble at 2x valuation. I love it at the right price.
$EXAI is a company that I own that is working on AI across all stages of drug discovery. They have 2 platforms with single cell biology genomics and AI drug discovery.
Read 6 tweets

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!

Ethereum

0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy

Bitcoin

3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us!

:(