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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Oct 30, 2021 • 30 tweets • 13 min read • Read on X
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1. Ariadne's Thread - SARS Vaccines

Collection of SARS Vaccine papers for Maze addicts

How was the Minotaur born?
2. Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins
Peng Zhou,Zhenggang Han,Lin-Fa Wang, Zhengli Shi

ncbi.nlm.nih.gov/labs/pmc/artic…
3. Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus

Yu-xuan Hou,Cheng Peng, Zheng-gang Han, Peng Zhou, Ji-guo Chen, Zheng-li Shi

1https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7090579/
4. Following the thread
5. Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
6. Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

ncbi.nlm.nih.gov/labs/pmc/artic…
7. A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
8. Unravelling the Thread
9. Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

ncbi.nlm.nih.gov/labs/pmc/artic…
10. Vaccine Design for Severe Acute Respiratory Syndrome Coronavirus

liebertpub.com/doi/10.1089/vi…
11. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
12. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
13. Minotaur emerges
14. Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of SARS-CoV Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection

ncbi.nlm.nih.gov/labs/pmc/artic…
15. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice (WIV, 2010)

ncbi.nlm.nih.gov/labs/pmc/artic…
16. Easy for a Mouse
17. A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response upon Challenge

ncbi.nlm.nih.gov/labs/pmc/artic…
For whom does the Bell Toll?

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

ncbi.nlm.nih.gov/labs/pmc/artic…
19. For Ferrets and Monkeys!

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

ncbi.nlm.nih.gov/labs/pmc/artic…
20. SARS-CoV Spike Proteins Expressed by the Vaccinia Virus Tiantan Strain: Secreted SQ Protein Induces Robust Neutralization Antibody in Mice

liebertpub.com/doi/10.1089/vi…
21. Chimeric Minotaur
22. Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV

ncbi.nlm.nih.gov/labs/pmc/artic…
23. Development of subunit vaccines against severe acute respiratory syndrome
Du, L., He, Y., Jiang, S., Zheng, B.-J.

journals.prous.com/journals/servl…
24. The Shadow
25. Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain

pubmed.ncbi.nlm.nih.gov/15640862/
27. The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

ncbi.nlm.nih.gov/labs/pmc/artic…
28. Truncated Recombinations, a Mouse's Nightmare
29. More
1. Studies of SARS vaccines
hkmj.org/abstracts/v14n…
2. Animal coronavirus vaccines: lessons for SARS
pubmed.ncbi.nlm.nih.gov/15742624/
3. SARS Vaccine Development
ncbi.nlm.nih.gov/labs/pmc/artic…
4.Vaccine Design for SARS
liebertpub.com/doi/10.1089/vi…
5. SARS Vaccination
cmi.ustc.edu.cn/1/3/193.pdf
30. From SARS 1 to SARS 2

A Review on SARS-CoV-2-Induced Neuroinflammation, Neurodevelopmental Complications, and Recent Updates on the Vaccine Development

ncbi.nlm.nih.gov/labs/pmc/artic…
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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Sep 11
1. "We’ll be seen as spies, not scientists.”

x.com/emilyakopp/sta…

Daszak "seems" confused when questioned BY @RepJamesComer whether he ever worked with, or for, spooks.

Unsurprisingly in light of what is shown on page 79 of the DRASTIC EHA files

researchgate.net/publication/39… x.com/Dylan_Housman/…Image
2. He may claim it was post pandemic contact

But thanks to @AGHuff we know it went back quite a while, at least to 2015.

@AGHuff 3. More questions from @NameIsSpartacus

We need to find out why Peter Daszak & EcoHealth had In-Q-Tel backing and why Nathan Wolfe (an obvious intelligence asset with links to Epstein/Maxwell, Boris Nikolic, and Bill Gates) was on their editorial board.

Read 14 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Sep 10
Good to see Generation Z in Asia growing a set of balls and fighting back against shit governments.



#NepalGenZProtest #Myanmar #SriLanka

Hopefully #China will be next!
Just 3 years ago #SrilankaProtest

One year ago #BangladeshProtest

Read 10 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Sep 6
🧵My comments and some conclusions regarding the EHA documents we found:

1. Peter Daszak actively schemed to foil congressional and other investigations into EcoHealth Alliance (EHA) and its ties to the Wuhan Institute of Virology, as revealed in the DRASTIC document stash.
2⃣ He orchestrated a “Coalition of Support” with former USAID officials like Dennis Carroll and NGOs like the Smithsonian, which sent letters (e.g., April 24, 2020, to NIH) decrying grant cuts as political attacks to deflect scrutiny (Part 6, p.35-36).
3⃣ Daszak hired the Kyle House Group (KHG) to lobby against NDAA defunding amendments, with KHG’s Porter joining meetings like one on August 4, 2023, with Rep. Adam Smith’s staff to kill Sen. Joni Ernst’s provisions (Part 7, p.52).
Read 16 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 22
1. Nord Stream Update

3 years for truth to emerge

@MerlynThomas on 27/09/22 for @BBCWorld

Ukraine accuses Russia of pipeline terror attack

bbc.com/news/world-eur…

@JamesCrisp6 on 28/09/22 for @Telegraph

Why Putin would want to blow up Nord Stream 2

archive.ph/JCtYB x.com/Panchenko_X/st…
2. Hilariously

Merlyn Thomas has been working for the "BBC disinformation unit" since September 2021.

He certainly earned his UK STATE propaganda pay check with Nord Stream!

3. Invitation to venture down a Baltic rabbit hole!

You are hereby invited to read my 682 tweet thread investigating all angles of the Nord Stream sabotage, which ends here:



Thanks @Erkperk @VasilijProzorov @JeffreyBrodsky5 @MichaKobs @bopanc @liscer72
Read 4 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets

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