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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Oct 30, 2021 • 30 tweets • 13 min read • Read on X
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1. Ariadne's Thread - SARS Vaccines

Collection of SARS Vaccine papers for Maze addicts

How was the Minotaur born?
2. Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins
Peng Zhou,Zhenggang Han,Lin-Fa Wang, Zhengli Shi

ncbi.nlm.nih.gov/labs/pmc/artic…
3. Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus

Yu-xuan Hou,Cheng Peng, Zheng-gang Han, Peng Zhou, Ji-guo Chen, Zheng-li Shi

1https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7090579/
4. Following the thread
5. Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
6. Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

ncbi.nlm.nih.gov/labs/pmc/artic…
7. A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
8. Unravelling the Thread
9. Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

ncbi.nlm.nih.gov/labs/pmc/artic…
10. Vaccine Design for Severe Acute Respiratory Syndrome Coronavirus

liebertpub.com/doi/10.1089/vi…
11. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
12. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
13. Minotaur emerges
14. Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of SARS-CoV Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection

ncbi.nlm.nih.gov/labs/pmc/artic…
15. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice (WIV, 2010)

ncbi.nlm.nih.gov/labs/pmc/artic…
16. Easy for a Mouse
17. A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response upon Challenge

ncbi.nlm.nih.gov/labs/pmc/artic…
For whom does the Bell Toll?

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

ncbi.nlm.nih.gov/labs/pmc/artic…
19. For Ferrets and Monkeys!

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

ncbi.nlm.nih.gov/labs/pmc/artic…
20. SARS-CoV Spike Proteins Expressed by the Vaccinia Virus Tiantan Strain: Secreted SQ Protein Induces Robust Neutralization Antibody in Mice

liebertpub.com/doi/10.1089/vi…
21. Chimeric Minotaur
22. Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV

ncbi.nlm.nih.gov/labs/pmc/artic…
23. Development of subunit vaccines against severe acute respiratory syndrome
Du, L., He, Y., Jiang, S., Zheng, B.-J.

journals.prous.com/journals/servl…
24. The Shadow
25. Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain

pubmed.ncbi.nlm.nih.gov/15640862/
27. The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

ncbi.nlm.nih.gov/labs/pmc/artic…
28. Truncated Recombinations, a Mouse's Nightmare
29. More
1. Studies of SARS vaccines
hkmj.org/abstracts/v14n…
2. Animal coronavirus vaccines: lessons for SARS
pubmed.ncbi.nlm.nih.gov/15742624/
3. SARS Vaccine Development
ncbi.nlm.nih.gov/labs/pmc/artic…
4.Vaccine Design for SARS
liebertpub.com/doi/10.1089/vi…
5. SARS Vaccination
cmi.ustc.edu.cn/1/3/193.pdf
30. From SARS 1 to SARS 2

A Review on SARS-CoV-2-Induced Neuroinflammation, Neurodevelopmental Complications, and Recent Updates on the Vaccine Development

ncbi.nlm.nih.gov/labs/pmc/artic…
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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 29
GUESS WHO SAID THIS?

"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?

@janeqiuchina

She continued:

"I was equally shocked by Daszak’s constant self-promotion and how effective it was." Image
@janeqiuchina About the film:

"Blame: Bats, Politics & a Planet Out of Balance"

"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda" Image
Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 28
Sebastian Castellio (1551)

To seek truth & to utter what one believes to be true can never be a crime. No one must be forced to accept a conviction

Conviction is free

One of the first proponents of religious toleration, freedom of conscience & thought

gospeltruth.net/zweig/heresy_c…Image
One of the great forgotten men of the Reformation.

One of the greatest minds in Geneva, a brilliant scholar and thinker.

Sebastian Castellio published what has been called "the first manifesto in favor of toleration"

web.archive.org/web/2005030616…
The Plague in Geneva

At the same time, he was the only minister in Geneva during Calvin’s day to risk his life to assist the sick during the Black Plague.

The friend of Truth obeys not the multitude, but the Truth.”

evangelicalarminians.org/sebastian-cast…
Read 11 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 28
🧵Latest SAGO Report

1. A good summary by @mattwridley but let's look at some details from the report, available here:

cdn.who.int/media/docs/def… x.com/R_H_Ebright/st…
2, Conflicts of Interest and Bias

Summed up by @R_H_Ebright

@R_H_Ebright 3. SAGO history and our struggle (January 2022)

Read 20 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 24
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.

Time to spend some of this tax money on local electronics, biological, ecological, IT, optical, chemistry, engineering & physics labs run by local amateurs for the benefit of our communities?
DRASTIC MEASURES NEEDED

Finally #scamdemia is waking up to the putrid stench of education for profit, ruined lives and academic credential inflation?

Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 9
Short 🧵on Primer & PCR Test issues (Pangolin Covs)

1. The key issue with pangolins and Pcovs is that:

It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless

The samples are retested with bespoke primers & more accurate PCR tests available now,

We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.

see Nga et al., 2022 for details

frontiersin.org/journals/publi…Image
3. It is unlikely that anyone will do that now😒

However, "they" (I will name who they are later) should, and perhaps in the future, may retest the stored samples at WIV, IPB, SCAU AND GIABR, to clarify the question of which coronaviruses were present in their pangolin samples.
Read 22 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 4
1. Tip of the Riceberg?

Not to mention wheat, barley and maize!

Read the disturbing criminal compaint here:

wlns.com/wp-content/upl… x.com/danaparish/sta…
2. May have been for research and commercial purposes, but they were caught bang to rights.

"LIU initially stated that he did not know what the materials were and that someone must have put them into his bag" Image
3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum
Read 17 tweets

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