1. Ariadne's Thread - SARS Vaccines

Collection of SARS Vaccine papers for Maze addicts

How was the Minotaur born?
2. Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins
Peng Zhou,Zhenggang Han,Lin-Fa Wang, Zhengli Shi

ncbi.nlm.nih.gov/labs/pmc/artic…
3. Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus

Yu-xuan Hou,Cheng Peng, Zheng-gang Han, Peng Zhou, Ji-guo Chen, Zheng-li Shi

1https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7090579/
4. Following the thread
5. Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
6. Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

ncbi.nlm.nih.gov/labs/pmc/artic…
7. A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
8. Unravelling the Thread
9. Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

ncbi.nlm.nih.gov/labs/pmc/artic…
10. Vaccine Design for Severe Acute Respiratory Syndrome Coronavirus

liebertpub.com/doi/10.1089/vi…
11. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
12. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
13. Minotaur emerges
14. Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of SARS-CoV Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection

ncbi.nlm.nih.gov/labs/pmc/artic…
15. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice (WIV, 2010)

ncbi.nlm.nih.gov/labs/pmc/artic…
16. Easy for a Mouse
17. A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response upon Challenge

ncbi.nlm.nih.gov/labs/pmc/artic…
For whom does the Bell Toll?

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

ncbi.nlm.nih.gov/labs/pmc/artic…
19. For Ferrets and Monkeys!

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

ncbi.nlm.nih.gov/labs/pmc/artic…
20. SARS-CoV Spike Proteins Expressed by the Vaccinia Virus Tiantan Strain: Secreted SQ Protein Induces Robust Neutralization Antibody in Mice

liebertpub.com/doi/10.1089/vi…
21. Chimeric Minotaur
22. Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV

ncbi.nlm.nih.gov/labs/pmc/artic…
23. Development of subunit vaccines against severe acute respiratory syndrome
Du, L., He, Y., Jiang, S., Zheng, B.-J.

journals.prous.com/journals/servl…
24. The Shadow
25. Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain

pubmed.ncbi.nlm.nih.gov/15640862/
27. The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

ncbi.nlm.nih.gov/labs/pmc/artic…
28. Truncated Recombinations, a Mouse's Nightmare
29. More
1. Studies of SARS vaccines
hkmj.org/abstracts/v14n…
2. Animal coronavirus vaccines: lessons for SARS
pubmed.ncbi.nlm.nih.gov/15742624/
3. SARS Vaccine Development
ncbi.nlm.nih.gov/labs/pmc/artic…
4.Vaccine Design for SARS
liebertpub.com/doi/10.1089/vi…
5. SARS Vaccination
cmi.ustc.edu.cn/1/3/193.pdf
30. From SARS 1 to SARS 2

A Review on SARS-CoV-2-Induced Neuroinflammation, Neurodevelopmental Complications, and Recent Updates on the Vaccine Development

ncbi.nlm.nih.gov/labs/pmc/artic…
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More from @BillyBostickson

Jul 9
CHINA MUST PAY!

The final finishing touches have now been added

Heritage Foundation Nonpartisan Commission on China and COVID-19

Report



PDF version



With Credits to DRASTIC & @daoyu15 & @netpoette

h/t @gdemaneuf @JamieMetzl heritage.org/china/report/h…
heritage.org/sites/default/…



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The Conclusion Image
More Credits where Credits are due, to:

@C_Small_
@billybostickson
@gdemaneuf


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Read 12 tweets
Jul 5
Are you interested in Traub's life & experiments?

1. He had a long, rich history & expertise in creating stealth biological weapons that evade tests, confuse diagnosis, & torment the afflicted with slow, chronic diseases, neurological problems & cancers

thesleeperagent.com/post/the-myste…
2. Biological Agents

"He specialized in developing incapacitating biological agents and mastered the art of this new class of germ weapons that were nearly incurable and caused devious effects on the afflicted"

thesleeperagent.com/post/immune-to…
3. Immune Tolerance

"Stealth biological weapons that attack the core of your being, that do not show up in diagnostic tests nor show any abnormal blood work that doctors use to diagnose, & without the level of inflammation that doctors can see in scans"

thesleeperagent.com/post/the-dark-…
Read 20 tweets
Jun 18
1. THE #DRASTIC #BIOSAFETY LAB MAP

Welcome to our new "uncensored" worldwide Map of BSL 3 & 4 Laboratories!

Soon to include epiwatch data & search functions to detect new epidemics in real time!

#DRASTIC Work in Progress!

google.com/maps/d/viewer?…
2. Our basic lab map has finally been launched

Specifically it is being posted publicly so that people can contribute to improving it

Remember, we are DECENTRALISED & AUTONOMOUS, unlike the well funded competition (£110,000)!

We are in this together!

kcl.ac.uk/news/project-a…
3. The "Other Map"

Count the dots



Koblenz and @FilippaLentzos



have done a great job with their well funded project

while ours was built collaboratively with no funding by people using old laptops ;)


scholar.google.com/citations?user…
kcl.ac.uk/warstudies/ass…
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Read 12 tweets
May 4
1. Heinz Felfe was head of the Soviet counter-intelligence Department & ran a long running project called "Panoptikum" to uncover a high level "mole" within the West German Intelligence Service...

In the end, the target of "Panoptikum" would turn out to be Heinz Felfe himself! Image
2. His Memoirs (in German)

Heinz Felfe published his memoir in 1986 under the title "In the Service of the enemy: Ten years as Moscow's man in the Federal Intelligence Service"

annas-archive.org/md5/837f80c065…
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3. Arrested in 1961, Exchanged in 1969

He gave the Soviets identities of 94 West German overseas "field officers", including the agency chief in Bangkok.

Here photographed at the moment of his exchange at a border checkpoint between West & East Germany in 1969 Image
Read 8 tweets
Mar 22
Disinformation from @jhas5

Don't take my word for it!

DYOR with Google Search 2022-2024

TRANSMISSION of SARS-COV-2 in:

Cats ✅
Ferrets ✅
hACE2 mice ✅
Syrian Golden hamsters ✅
(many at WIBP next to WIV)
Macaques ❓

See



and

sciencedirect.com/science/articl…
msdvetmanual.com/generalized-co…

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Cats have 9 lives

1. "SARS-CoV-2 transmission between cats is efficient and can be sustained,”

Efficient Direct & Limited Environmental Transmission of SARS-CoV-2 Lineage B.1.22 in Domestic Cats

journals.asm.org/doi/10.1128/sp…
2. "Genetic study supported the hypothesis of SARS-CoV-2 transmission from the owner to the cat, and then from the cat to the veterinarian"

Suspected Cat-to-Human Transmission of SARS-CoV-2, Thailand, July–September 2021

ncbi.nlm.nih.gov/pmc/articles/P…
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Read 11 tweets
Mar 20
1, What we proposed in 2021

"During initial stages of a forensic investigation of Wuhan Labs, all 4 evaluation tools including Grunow & Finke's epidemiological assessment criteria would be useful adjuncts to the investigation framework of Pilch et al."

researchgate.net/publication/35…



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2. Later in 2021

"The Radosavljevic–Belojevic method for outbreak scoring & differentiation shows that official assumptions of its natural origin is questionable & highlights the probability that the pathogen was "accidentally" introduced to humans"

3. Let's Go back to Stalingrad ;)

There were disputes about Tularemia in 2001

Tularemia, Biological Warfare & the Battle for Stalingrad (1942–1943)

Click on PDF



and in 2005

Alibek, Tularaemia and The Battle of Stalingrad

academic.oup.com/milmed/article…
researchgate.net/publication/24…

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Read 19 tweets

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