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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Oct 30, 2021 • 30 tweets • 13 min read • Read on X
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1. Ariadne's Thread - SARS Vaccines

Collection of SARS Vaccine papers for Maze addicts

How was the Minotaur born?
2. Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins
Peng Zhou,Zhenggang Han,Lin-Fa Wang, Zhengli Shi

ncbi.nlm.nih.gov/labs/pmc/artic…
3. Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus

Yu-xuan Hou,Cheng Peng, Zheng-gang Han, Peng Zhou, Ji-guo Chen, Zheng-li Shi

1https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7090579/
4. Following the thread
5. Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
6. Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

ncbi.nlm.nih.gov/labs/pmc/artic…
7. A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
8. Unravelling the Thread
9. Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

ncbi.nlm.nih.gov/labs/pmc/artic…
10. Vaccine Design for Severe Acute Respiratory Syndrome Coronavirus

liebertpub.com/doi/10.1089/vi…
11. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
12. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
13. Minotaur emerges
14. Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of SARS-CoV Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection

ncbi.nlm.nih.gov/labs/pmc/artic…
15. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice (WIV, 2010)

ncbi.nlm.nih.gov/labs/pmc/artic…
16. Easy for a Mouse
17. A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response upon Challenge

ncbi.nlm.nih.gov/labs/pmc/artic…
For whom does the Bell Toll?

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

ncbi.nlm.nih.gov/labs/pmc/artic…
19. For Ferrets and Monkeys!

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

ncbi.nlm.nih.gov/labs/pmc/artic…
20. SARS-CoV Spike Proteins Expressed by the Vaccinia Virus Tiantan Strain: Secreted SQ Protein Induces Robust Neutralization Antibody in Mice

liebertpub.com/doi/10.1089/vi…
21. Chimeric Minotaur
22. Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV

ncbi.nlm.nih.gov/labs/pmc/artic…
23. Development of subunit vaccines against severe acute respiratory syndrome
Du, L., He, Y., Jiang, S., Zheng, B.-J.

journals.prous.com/journals/servl…
24. The Shadow
25. Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain

pubmed.ncbi.nlm.nih.gov/15640862/
27. The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

ncbi.nlm.nih.gov/labs/pmc/artic…
28. Truncated Recombinations, a Mouse's Nightmare
29. More
1. Studies of SARS vaccines
hkmj.org/abstracts/v14n…
2. Animal coronavirus vaccines: lessons for SARS
pubmed.ncbi.nlm.nih.gov/15742624/
3. SARS Vaccine Development
ncbi.nlm.nih.gov/labs/pmc/artic…
4.Vaccine Design for SARS
liebertpub.com/doi/10.1089/vi…
5. SARS Vaccination
cmi.ustc.edu.cn/1/3/193.pdf
30. From SARS 1 to SARS 2

A Review on SARS-CoV-2-Induced Neuroinflammation, Neurodevelopmental Complications, and Recent Updates on the Vaccine Development

ncbi.nlm.nih.gov/labs/pmc/artic…
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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 19
1. Russian Biolab Claims: A Controversial Topic

Good luck using your critical thinking skills to seperate wheat from chaff so that you can decide what is propaganda & what is fact.

Of course the easy options are to dismiss everything as lies or to swallow everything as fact.
2. What I recommend

If you are interested in a balanced analysis of the Russian claims and all the detailed evidence presented by Kirillov before his assassination in Moscow, as well as relted topics, then I highly recommend you plough through those threads at your leisure.
3. Documents & Russian claims on Projects UP-4 (avian flu) Flu-Flyway (wild ducks & bird flu), P-781 (bat coronaviruses), UP-8 (CCHF, Leptospirosis Hanta viruses):

Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 15
1. 'CONSPIRACY OF SILENCE"

Pentagon was told 6 times that Covid was lab leak at start of pandemic – but buried the truth, ex-intel official says.

Story now published with intriguing detail by @imogen_braddick after we informed her of John Myers Tweet

thesun.co.uk/news/32257526/… x.com/BillyBostickso…
2. John Myers said that:

he "knows with absolute certainty" that top US military chiefs were told Covid had leaked from a lab in Wuhan.

"the Joint Chiefs of Staff were briefed in October 2019 - more than five years ago - about a new virus that had leaked from a lab in China"
3. Claims

This was briefed in Oct& Nov 2019 as a lab leak.

It was in the intelligence. We briefed it. It was accepted. I briefed it numerous times about a viral outbreak and that it was from a lab.

"Over the course of late Nov & Dec, it came up 6 or 7 times in briefings.
Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 12
🧵📸 We have now solved the Pangolin Riddle!

This is BIG so sit tight!

1. WIV did have Pangolins in 2019 (we now have proof)

2. WIV isolated Pangolin/HKU4/MERS like Covs with Furin Sites in 2019, just before the pandemic!
3. Important Note

If you have not done so already, please read the linked thread above before continuing, it will make much more sense if you do so.
4. GX Pangolins at WIV in 2019!

From the Horse's Mouth in 2024!

"In 2019 & 2020, 86 anal swab samples & 80 serum samples were collected from Guangxi pangolins"

"Pangolin samples were transported & stored at Wuhan Institute of Virology, CAS"

cell.com/cell/fulltext/…Image
Read 53 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 11
Missing Documents from EHA & Peter Daszak

He hid all WeChat comms with WIV & Zhengli Shi from @COVIDSelect

However, EHA provided the #DOJ subpoena with tens of thousands of unredacted comms stretching back a decade.

AND where are these OIG audits:

#DHHS
@USGAO
@NIH
@USAID
1. @COVIDSelect @mitch_benzine @RepBradWenstrup

Have much more unreleased information

@COVIDSelect @mitch_benzine @RepBradWenstrup 2. DOJ Audit

1. "DOJ subpoenaed EcoHealth’s communications (inlcuding WeChat) with, at least, Dr. Shi. This subpoena included both Dr. Shi’s official WIV email address & her Hotmail"

2. “DoJ subpoena for genetic sequences, docs — almost complete”

nypost.com/2024/12/02/us-…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 11
Pangolin Riddle!

MpCoV-GX Isolated in 2017 said YiGang Tong

BUT ZLS & Yi GangTong in 2023 said:

"MpCoV-GX was isolated in 2019"

SARS-CoV-2-Related Virus from Malayan Pangolin

pmc.ncbi.nlm.nih.gov/articles/PMC99…

Accession data says submission date is 8 FEB 2020

ncbi.nlm.nih.gov/nuccore/MT0403… x.com/BillyBostickso…
2. Double Confirmed

Yi Gang Tong confrimed again the 2017 isolation date for MpCov-GX.

He experimented with it, but did not write a paper, and they did not upload the sequence until 8th Feb 2022, just after the beginning of the SARS-COV-2 pandemic

3. Why is this significant?

Yi Gang Tong stated in a paper received on 22 Feb 2020:

"no suspected infections were found in those having close contacts with pangolins"

"Our pangolin coronavirus isolate was routinely cultured in BSL 2 FACILITIES"

Read 20 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Nov 29
Most interesting RaTG13 - SARS-COV 2 paper ever written by Chinese Scientists!

Received 17 July 2020 before Gag Orders kicked in.

Comparative Genomic Analyses Reveal Specific Mutation Pattern Between SARS-CoV-2 & RaTG13

frontiersin.org/journals/micro…

See Tweeets 32 to 35 for Why 👇 x.com/BillyBostickso…
2. Potential Effects of 5-FU

Use of 5-FU during serial passage could lead to increased mutation rates in RNA viruses which might result in variants in enhanced virulence.

However, this is not guaranteed; many mutations can be deleterious rather than beneficial.
3. Using 5-FU

5-fluorouracil can induce mutations during serial passage of RNA viruses that could lead to variants with altered virulence characteristics.

Outcome would depend on specific virus involved, the nature of induced mutations & environmental pressures during passage. Image
Read 6 tweets

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