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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Oct 30, 2021 • 30 tweets • 13 min read • Read on X
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1. Ariadne's Thread - SARS Vaccines

Collection of SARS Vaccine papers for Maze addicts

How was the Minotaur born?
2. Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins
Peng Zhou,Zhenggang Han,Lin-Fa Wang, Zhengli Shi

ncbi.nlm.nih.gov/labs/pmc/artic…
3. Immunogenicity of the spike glycoprotein of Bat SARS-like coronavirus

Yu-xuan Hou,Cheng Peng, Zheng-gang Han, Peng Zhou, Ji-guo Chen, Zheng-li Shi

1https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7090579/
4. Following the thread
5. Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
6. Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

ncbi.nlm.nih.gov/labs/pmc/artic…
7. A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

ncbi.nlm.nih.gov/labs/pmc/artic…
8. Unravelling the Thread
9. Antigenicity and immunogenicity of SARS-CoV S protein receptor-binding domain stably expressed in CHO cells

ncbi.nlm.nih.gov/labs/pmc/artic…
10. Vaccine Design for Severe Acute Respiratory Syndrome Coronavirus

liebertpub.com/doi/10.1089/vi…
11. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
12. Recombinant Modified Vaccinia Virus Ankara Expressing the Spike Glycoprotein of Severe Acute Respiratory Syndrome Coronavirus Induces Protective Neutralizing Antibodies Primarily Targeting the Receptor Binding Region

ncbi.nlm.nih.gov/labs/pmc/artic…
13. Minotaur emerges
14. Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of SARS-CoV Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection

ncbi.nlm.nih.gov/labs/pmc/artic…
15. Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice (WIV, 2010)

ncbi.nlm.nih.gov/labs/pmc/artic…
16. Easy for a Mouse
17. A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response upon Challenge

ncbi.nlm.nih.gov/labs/pmc/artic…
For whom does the Bell Toll?

Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine

ncbi.nlm.nih.gov/labs/pmc/artic…
19. For Ferrets and Monkeys!

Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

ncbi.nlm.nih.gov/labs/pmc/artic…
20. SARS-CoV Spike Proteins Expressed by the Vaccinia Virus Tiantan Strain: Secreted SQ Protein Induces Robust Neutralization Antibody in Mice

liebertpub.com/doi/10.1089/vi…
21. Chimeric Minotaur
22. Chimeric severe acute respiratory syndrome coronavirus (SARS-CoV) S glycoprotein and influenza matrix 1 efficiently form virus-like particles (VLPs) that protect mice against challenge with SARS-CoV

ncbi.nlm.nih.gov/labs/pmc/artic…
23. Development of subunit vaccines against severe acute respiratory syndrome
Du, L., He, Y., Jiang, S., Zheng, B.-J.

journals.prous.com/journals/servl…
24. The Shadow
25. Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain

pubmed.ncbi.nlm.nih.gov/15640862/
27. The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

ncbi.nlm.nih.gov/labs/pmc/artic…
28. Truncated Recombinations, a Mouse's Nightmare
29. More
1. Studies of SARS vaccines
hkmj.org/abstracts/v14n…
2. Animal coronavirus vaccines: lessons for SARS
pubmed.ncbi.nlm.nih.gov/15742624/
3. SARS Vaccine Development
ncbi.nlm.nih.gov/labs/pmc/artic…
4.Vaccine Design for SARS
liebertpub.com/doi/10.1089/vi…
5. SARS Vaccination
cmi.ustc.edu.cn/1/3/193.pdf
30. From SARS 1 to SARS 2

A Review on SARS-CoV-2-Induced Neuroinflammation, Neurodevelopmental Complications, and Recent Updates on the Vaccine Development

ncbi.nlm.nih.gov/labs/pmc/artic…
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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 3
1. Remote truck drone swarm bombings are here to stay!

The techniques will be improved and nowhere will be safe unless underground.

Questions to consider:

How many panel vans, transit vans and small trucks are there in a big city at any one time?
2. This is a revolution in sabotage tactics



Welcome to the future of air bases!

Without concrete and earth roofed revetments, aircraft are sitting ducks.kyivpost.com/post/53749
3. The "good old days"

"clandestine antiaircraft devices using gallium metal pencils which when applied to aircraft results In destruction due to stress failure at the point of application"

osti.gov/opennet/servle…

Use & Effectiveness of Sabotage (1987)

apps.dtic.mil/sti/tr/pdf/ADA…
Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
May 29
As we edge faster and faster into the future, the future

Why not travel back in time, a mere one hundred years?

Thanks to the @internetarchive

Take a moment to digest the news and advertisements found in the Hong Kong Daily Press 28th of May 1925

archive.org/details/NPDP19…
Some stories!

GREAT REVOLT SCHEME. SECRET PLANS OF THE THIRD INTERNATIONAL. BOLSHEVISING BULGARIA. A BALKAN FRONT. SPENDTHRIFT MURDERER. SPENDS AMILLION FRANCS IN FOURTEEN DAYS. MENUS OF LONDON'S ZOO. MR. CHURCHILL'S BUDGET. HAKKAS AND HOKLOS. PITCHED BATTLE AT PING CHAU.
More stories!

CANTON SITUATION. SPORT IN THE FAR EAST. GERMAN DISARMAMENT. BRITISH SILK DUTIES. COTTON SPINNERS. WORKING WEEK DISPUTE NOT YET SETTLED. FATE OF AMUNDSEN. MR. HAMMER IS MAKING PLANS FOR RESCUE. THE SOFIA OUTRAGE. POSITIONS FOR TROTZKY. THE EPSOM DERBY.
Read 34 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
May 22
🧵1. Another Modest Proposal

This time for the Pangolin’s Noble Sacrifice!

In this enlightened year of our Lord 2025 I, a humble observer of mankind’s ceaseless quest for health, find myself much struck by the ingenious practices of the Orient..
🧵2. A Scaly Panacea

...where the pangolin, that scaly denizen of subtropical climes, hath been elevated to a panacea most wondrous.

The Baicao Chinese Medicine compendium, a veritable gospel of herbal wisdom, doth proclaim the virtues of this creature’s scales...
🧵3. Humanity's Salvation?

...harvested with a diligence that would shame the most ardent of alchemists.

I propose, therefore, a modest scheme to amplify this noble tradition, ensuring that the pangolin’s sacrifice might serve the greater good of humanity.
Read 16 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
May 21
🧵🗞️The International News

A Convulsive Horizon of No Escape

Clang! Bar, far, scar, star.

Take the swarming headlines, shred them,

South Korea’s soy sauce grand master stirs

Books that don’t exist, AI’s fevered brew.

War without purpose, war crimes bite,
🧵🗞️ Clang! Crimes, limes, slimes!

EU’s chocolate crisis, cocoa beans scream!

Climate breakdown bites the fields.

The moment that changed me

a boulangerie revelation melting into Vindolanda’s stone relief

But the man in the navy suit and tie,

chokes on known unknowns.
🧵🗞️ Clang! Rice, nice, vice, dice!

Japanese minister’s rice is free, free, glee, spree,

As Trump’s tariffs tangle with toilet roll wedding lists.

No escape. The hour will no longer be ours.

Clang! Dome, home, foam, roam!
Read 38 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
May 16
Some insights into our DRASTIC investigation

1. What appears wasteful research to you in the present may be of great value to you or others in the future, if it is given the time to develop to its logical end.

Don’t judge the usefulness of your findings too quickly!
2. What appears wasteful or a dead end to you..

May be of immense value to somebody else!

Don’t judge the usefulness:

1. Of other people’s findings based only on your own assessment of them.

2. Of your own findings based only on your own current assessment of them either.
3. What appears wasteful and a dead end to you..

A. May be the raw stuff that new hypotheses and new research directions are born from.

B. Don’t judge the usefulness of your "investigations" until you have given them enough time to surprise you.
Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
May 13
🧵Holmes Studies "Lab Leak"🤔

1⃣ WIV

-Deliberate release of bioweapon
-Accidental release of bioweapon in development
-Lab accident during genetic engineering
-Lab accident during cell culture
-Lab accident during vaccine development
-Accidental infection that no one knew about
2⃣ WCDC

• Lab worker infected during field animal sampling
• Accident during experimental work
• Accident from exposure to infected waste
• Accident during move to new location
• WCDC gave bat samples to the WIV
3⃣ Virus engineering

•Engineered from RaTG13
•Engineered from pool of bat viruses (Laos)
•Engineered from secret database of bat viruses
•Engineered at UNC & shipped to the WIV
•Engineered hybrid of bat & pangolin viruses🐵
•Synthetic infectious clone engineered in vitro
Read 8 tweets

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