Recent @WhiteCoatWaste FOIA release

Laos to WIV, 2018!

"LAOS PI or Co-Investigators to conduct field
trip to assess markets, identify wildlife in them & arrange for shipment of bats and other high-risk host species to WIV Laboratory in China"

scribd.com/document/53702…
All Roads & Bats lead to the Wuhan institute of Death
If PD won't tell us, then these people need to be asked some questions about exactly aht samples they sent to WIV.
Wuhan University..
The usual suspects
Risks of Exposure to Coronavirus Infection

Aleksei Chmura (2015):

"We do not have formal plans to provide participants with information about minimizing risks of exposure to Coronavirus infection"
DoD, July 2019
To be more precise....Department of the Navy

Remember the LAOS expedition in 2017?

US DoD and Pasteur Institute were in Feuang District where the closest bat coronavirus, Banal 20 52, came from, back in 2017

2016, Page 378

"In addition, your progress report makes reference to two chimeric bat SARS-like CoVs constructed on a WIV-1 backbone"

Fast forward to 2018

"Pathogenicity of 2 new bat SARS-related covs to transgenic mice expressing human ACE2"

Signing off for now with the Wuhan University ABSL3

More on that particular laboratory here:

Pages 5 to 17

researchgate.net/publication/35…

"Experimental work using humanized mice will be
conducted at the Center for Animal Experiment Biosafety 3 lab of Wuhan University"
Lab Mice also at WIV ABSL3! (P. 234)

Laboratory mice will be housed in the BSL-3 small animal facility Center for Animal Experiment
at Wuhan Institute of Virology . Two senior Wuhan Institute of Virology veterinarians (Drs. An XueFang and Zhang Fan) will oversee the experiments"
Why we need to see Dr. Daszak's Emails (P. 234)

"The Veterinarian in charge will notify the on-site Co-P l (Dr. Zheng li Shi) and the Principal Investigator (Dr. Daszak) by telephone and email if there are any issues regarding animal health and welfare"
Ben Hu's grant, for sure (Page 18)

Pathogenicity studies of 2 novel bat SARSr-CoVs on transgenic mice expressing human ACE2""
Source of Support: National Natural Science Foundation of China
Award Amount: $44,776
01/01/2019 -12/31/2021



via @TylerBullit
Bad Idea (Page 19)

Interferon responses in SARS - Like Coronavirus infected Bat cells

National Basic Research program of China

Total Award Amount: $100,298

01/0 1/2018-12/31 /2022

Location of Project: Wuhan Institute of Virology, CAS
Why a bad idea, you may ask?

Explained here (Pages 53 and 54):

"unknown or undetected highly pathogenic bat viruses would have been able to replicate clandestinely in WIV bat cell lines as they would not have been constrained by a bat immune system"

researchgate.net/publication/35…
unroll updated FOIA Laos to WIV @threadreaderapp

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More from @BillyBostickson

Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets
Jul 1
🧵Rev. Billy's Lab Leak Cover-Up Sermon

1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!

The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!

Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!

And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception

Let us expose this falsehood, this house of deceit

& the duties of the vigilant in these perilous times!

Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
Read 23 tweets

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