Dr. rer. nat. Valentin Bruttel Profile picture
Dec 3, 2021 21 tweets 13 min read Read on X
@ydeigin So to sum this up:
1) SARS2 Omicron genome looks like it was "separated/frozen" between June 2020 and late summer 2021 -
This has never before been seen in nature, only in lab leaked pandemics
en.wikipedia.org/wiki/1977_Russ…
@ydeigin 2) SARS2 Omicron has 25:1 non-synonymous:synonymous mutations ratio.

Natural evolution would result in 25:50-100 ratios.
The only logical explanation here is targeted manipulation.
@ydeigin 3) No SARS2 Omicron predecessors have been detected between June 2020 and fall 2021, despite their supposedly already higher infectivity.
So in this time,
- a single isolated patient bred more mutations than >100Mio others?
- survived continous infection in complete isolation?
@ydeigin 4) Omicron is better adapted to humans and likely also to vaccinated/recovered humans.
If this was acquired in a large pool of humans, we would have seen predecessors of this variant before.
Evolution in animals should have resulted in a less-well adapted variant.
@ydeigin 5) The only plausible explanation how this huge number of mutations could have been acquired completely below the radar is:
- scientists enabled SARS2 samples to escape antibody responses by passaging them in presence of diluted convalescent serum on human/humanized cell lines.
@ydeigin 6) Exactly this extremely risky research, which could render all of world wide vaccination efforts useless, was done in South Africa.
nature.com/articles/s4158…
And not even at their BSL4 lab, no, in Durban, which only has a BSL3 lab😱. Image
@ydeigin 7) Durban, as you mentioned above @ydeigin, is exactly the city where omicron was first detected in one of the patients.
Where they "trained" SARS2 variants to become resistant to neutralizing antibodies by slowly and carefully increasing covalescent plasma concentrations... Image
@ydeigin 8) and not just any SARS2 varian, no the had sampled their viruses in JUNE 2020, when the closest omicron relative was last seen out there.
And not any variant, no, e.g. a B.1.1.117, B.1.1.1 and a B.1.1.56 virus.
Omicron is B.1.1.529, sounds somewhat similar, right? Image
@ydeigin 9) of course they used cell lines transfected for human ACE2, and froze their viral samples, which still is the only logical explanation for omicron completely splitting off from all other variants of in June 2020. Image
@ydeigin 10) IMO someone:
1. bred different antibody-escape-mutations w/ diluted convalescent plasma
2. maybe also wondered what happened if those were combined & made a recombinant AV.1. SARS2 variant with all these mutations for 3000$
4. inhaled some aerosols/dropped a flask
➡️ omicron Image
@ydeigin 11) The most sickening part is that all of this work could have been done with basically no risk by just putting the spike protein into a harmless pseudovirus.
Like done here:
nature.com/articles/s4158…
With strikingly similar results btw:
@ydeigin 12) These are just my thoughts Saturday morning 2.30am after an intense work week. Please correct me whereever I may be wrong.
This deserves a proper investigation that goes into the labs.
Fact is that virologists keep doing highly risky research at very questionable conditions. Image
@ydeigin 13) We must end this research, at least until we can develop safe international experimental, ethical and reporting guidelines and maybe move it to an isolated island.
Otherwise this research could just end us.

I'm out for now, live long and prosper🖖
@ydeigin 14) have to add a few points:
artificial viruses that acombine resistance-enhancing mutations from several strains are called polymutants:

Someone could have copied that strategy w/ real SARS2, or a pseudovirus spike could have leaked and recombined.
@ydeigin 15) high selective pressure + high viral loads can lead to pretty high non-silent:silent (dN:dS) mutation ratios.
In normal CoV-evolution, this is 0.1 to 0.2.
However, e.g. in immunocompromized patients (long term high viral loads) can increase to 2-3.
academic.oup.com/jid/article/22… ImageImage
@ydeigin 16) The very same should be true, if not even more so, when passaging CoVs in presence of antibodies/convalescent sera.
This study has generated synthetic polymutant SARS2 spikes with mutations generated through exactly this process, and even many mutations are identical: Image
@ydeigin 17) Thus far, we have only seen 1-7 spike mutations in immunocompromised patients, way less than in synthetic polymutants or omicron (33). ImageImage
@ydeigin 18) Also, in immunocompromised patients, nonsilent mutations seem to be less concentrated in the spike.
After all, such patients may not have sufficient antibodies to maintain selective pressure, as opposed to cell culture with finely tuned patient sera concentrations. Image
@ydeigin 19) I have also learned that even some of the lead scientists working on vaccine-resistant SARS2 spike proteins seem to still believe that lab leaks (en.wikipedia.org/wiki/List_of_l…) cannot happen.
Should we trust s.o. who hasn't heard of Chernobyl/Fukushima to run a nuclear power plant? Image
@ydeigin 20) IMO, lab leak is still the most plausible explanation for omicron.
Connects all the dots: location, isolations since ~06/2020, strong selective pressure on spike, not so safe lab...
But not 100% sure anymore it was engineered, maybe only passaging w/ patient sera?
Let's look!
@ydeigin correction: dN:dS in omicron spike alone could be even as high as 33:1, depending on the variant. Typical dN:dS is 1:5 (0.2) in stable sarbeco-viruses.
If this was natural, best explained by continuous passaging (high viral loads) with patient/vacc. sera (high sel. pressure). Image

• • •

Missing some Tweet in this thread? You can try to force a refresh
 

Keep Current with Dr. rer. nat. Valentin Bruttel

Dr. rer. nat. Valentin Bruttel Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

More from @VBruttel

Nov 2, 2023
🧵How few anonymous accounts censor Wikipedia with regards to the origin of SARS-CoV-2, and why you should supporting Wikipedia until this is resolved.
I love Wikipedia. It's a great place to start reading into new topics, to find relevant literature, to look things up.
1/
However, the page on the origin of SARS2 is highly misleading. I tried to improve it. And got censored. Here are some of the biggest problems, and why they are not getting resolved.
2/en.wikipedia.org/wiki/COVID-19_…
1) Wording/tone clearly not objective
A hypothesis based on very solid facts and observations becomes a an "idea". The term "conspiracy theory" comes up 36 times. The fact that quoted scientists believed a lab leak to be likely is completely ignored. Many editors are rude.
3/


Image
Image
Image
Image
Read 11 tweets
Jul 16, 2023
unpopular opinion:
no one on earth truly understands how vaccinations fully work, and which patient-dependent long-term side effects (MHC-dependent molecular mimicry) and unexpected benefits (local immune activation) they bring. let's start re-thinking tests and trial designs.
IMO there are 2 camps with an enormous trench in between. Some say vaccinations are safe, don't cause relevant side effect, some see vaccinations as the source of all evil. I do not agree with either side, and think we could all benefit from a serious scientific discussion.
I am not an expert on vaccinations, but have studied immune responses to antigens for >15 years. The work by @StabellBenn IMO clearly shows that vaccinations do more than just protect against the disease against which someone is vaccinated, and that vaccine formats matter a lot.
Read 10 tweets
Apr 8, 2023
Wie Deutsche Medien Ihren Lesern immer noch den größten Quatsch zum Ursprung von SARS-CoV-2 unterjubeln.
Heute die @DIEZEIT, Artikel von @flor8i und @ClaWuest:

(Gott sei dank mit paywall)
1/ https://t.co/XV9mwiOyeKzeit.de/gesundheit/202…
Image
Vorweg ist es wiedermal ein Riesenproblem, dass nicht über die MASSIVEN INTERESSENSKONFLIKTE von Eddie Holmes aufgeklärt wird. Holmes hat 2018 mit dem WIV eine 99,5% proteinidentische Virensequenz hochgeladen, irreführende Artikel veröffentlicht und verweigert Zugang zu Emails
/2


Image
Image
Image
Image
Achja, und Holmes in Gastprofessor am chinesischen CDC.

Das schrieb übrigens die wohl wichtigste non-profit Organisation zum Thema, Biosafety Now, über Holmes und seine Mitautoren:

/3 https://t.co/X4BR8alntwbiosafetynow.org/press-release-…

Image
Image
Read 21 tweets
Mar 25, 2023
Leaving Twitter

Dear Friend,
GoF research especially in the field of synthetic virology still is extinction level threat as @AshleyRindsberg recently phrased it.
thespectator.com/topic/funding-…
Despite constant ghost bans, Twitter was important to investigate the origin of SARS2.
1/
The recent #RaccoonDogHoax proved that
a) Zoonati have left the realms of objective, responsible scientific investigating &reporting.
Facts will not change their minds.
The truth is just too terrible.
b) There really is no evidence for a zoonotic origin
vbruttel.substack.com/p/the-stronges…
2/
The cumulative evidence, which many may not know or understand, proves a synthetic origin of SARS2 beyond reasonable doubt:

I am proud that @WashburneAlex and @tony_vandongen an I have contributed an important puzzle piece here
biorxiv.org/content/10.110…
3/
Read 12 tweets
Mar 21, 2023
@flodebarre @KatherineEban @JamesCTobias wait a minute - that's it?
are you calling it a report to avoid peer review or because you have no raw data?
where are the ancestral genomes?
what are the sample collection methods and time points?
all the fanfare - about virtually nothing?
here some super obvious weaknesses:
🧵
@flodebarre @KatherineEban @JamesCTobias 1st sentence wrong, the preponderance of according to Prof. Embarek likely thousands of early samples was never sequenced. Case screening specifically targeted people connected to HSM, all we can say is that it was a sampling hotspot. HSM samples are clearly not ancestral.
@flodebarre @KatherineEban @JamesCTobias again, the area with the mammals was just the most sampled area. if corrected for sampling frequency, most positive samples clearly correlate with were the toilets are
zenodo.org/record/7212687…
Read 19 tweets
Jan 21, 2023
Now this is interesting:

seems like a State Key Laboratory of Pathogen and Biosecurity scientist from Beijing uploaded a sequence from a 2019 Henan sputum sample. He thought it was from pseudomonas, but it is actually 100% the SARS2 spike without the FCS.
Remember, contaminations happen, this sequence could have come from another sample analysed in the same deep sequencing analysis.
This strongly indicates that a 100% SC2-identical spike (minus FCS) was worked with in 🇨🇳 in 2019.
Were 3nt (coding A) removed before adding the FCS?
I wrote worked with, as the rest of the seq. looks engineered:
-the first 21aa seem to come from a human plasminogen activator
-the following 8aa (GGGSGGGS) could be a flexible protein linker
-what's that IEK rich domain that follows?
Could this be a protein vaccine spike?
Read 19 tweets

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!

Ethereum

0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy

Bitcoin

3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us!

:(