An Examination of the Potential Threat of a State-Sponsored Biological Attack Against the United States

2019 Master's Thesis

bearworks.missouristate.edu/cgi/viewconten…Image
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This section refers to:

China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities (2015) by Danny Shoham

idsa.in/jds/9_2_2015_C…

PDF

idsa.in/system/files/j… Image
The author of the Master's Thesis, Corey Pfluke wrote a summarised version of her section on China in 2020:

Biohazard: A Look at China’s Biological Capabilities and the Recent Coronavirus Outbreak

airuniversity.af.edu/Wild-Blue-Yond…
"Although assessing China’s military capabilities is notoriously difficult due to the secrecy & opacity its institutions maintain, a combination of historical records, assessments, & studies provide a view of China’s BW programme, this view is unsettling"
orfonline.org/expert-speak/a…
An Indian Perspective (Part 1)

AN ASSESSMENT OF CHINA’S BIOLOGICAL WARFARE CAPABILITIES AND NEED FOR GLOBAL APPROACH TO BIO-SECURITY

researchgate.net/publication/34…
An Indian Perspective (Part 2)

AN ASSESSMENT OF CHINA’S BIOLOGICAL WARFARE CAPABILITIES AND NEED FOR GLOBAL APPROACH TO BIO-SECURITY

researchgate.net/publication/34…
An important Reference is a paper by Croddy (2002 )

(a little dated but with many useful references to suspected facilities)

including the Wuhan Institute of Biological Products

(WIBP..next door to the WIV BSL4 Complex in Jingxia)

nonproliferation.org/wp-content/upl…Image
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Hence my tweet yesterday



and

"126 tedious WIBP supply documents translated

A minor victory!"
Essential Background Reading on WIBP

Published by DRASTIC a year ago

researchgate.net/publication/35…
Referenced in the Taiwan News by @keverington

taiwannews.com.tw/en/news/4137284
Another article by Dany Shoham focusing on the Canadian-Tianjin Angle:

China's Biological Warfare Programme and the Curious Case of Dr. Xiangguo Qiu (2019)

idsa.in/cbwmagazine/ch…

x.com/BillyBostickso…
The Radosavljevic–Belojevic method for outbreak scoring & differentiation shows that the official assumption of its natural origin is questionable and highlights the probability that the pathogen may have been "accidentally" introduced to humans

researchgate.net/publication/35…
“The genomic origin of the strain that infected patient zero was a Chinese bat virus that underwent extensive pre-adaptation to humans, including continual transmissibility..
“The open question is how, where, and when such exceptional genomic pre-adaptation took place” Image
One possibility is that a wild-type virus was 1st propagated repeatedly in human tissue cultures & the resulting spontaneously upgraded virus was then used to experimentally infect monkeys or ferrets—one of which then accidentally infected staff in the lab
jns.org/opinion/where-…
An interesting observation

"The WIV has long been routinely supplied with rhesus monkeys from the Macaque Breeding Base in Suizhou City"

Note the references have been deleted:
safeboxasia.com/en/center/inde…
and
safeboxasia.com/en/news/info_9…

and replaced with chinese lottery website! Image
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Top Gene Bio's new cleaned website

topgenebio.com

Facebook Page:

facebook.com/topgenebio/

Invite you to search
Snapshots Image
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20,000 Caged rats and mice

800 + Monkeys Image
1300 Types of genetically engineered mice
300 types of genetically engineered rats Image
A well known supplier of Monkeys.... Image
"Business Segment" Image
Taking a break, to be continued.
The "chinese lottery" website can actually be accessed in all its tedious glory by installing a noscript extensión

safeboxasia.com/cn/animal/list…Image
You may be wondering why this thread has diverged into Chinese monkey breeding minutia...

Reason being that any putative Chinese BW program would require monkeys for experiments, as was the case in USA & UK (Operation Cauldron)

en.wikipedia.org/wiki/Operation…

bbc.com/news/uk-englan…
Operation Cauldron Film:

The trials were conducted by scientists from Porton Down in Wiltshire. About 3,500 monkeys and guinea pigs were killed after exposure to "plague bombs"

archive.org/details/Operat…

and

vimeo.com/50042014 Image
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Operation Cauldron, The Stuff of Monkey Nightmares

More Chinese Monkey Research and Breeding Programs to come tomorrow
5 species of primates are used in China for biomedical research
2 species, crab-eating macaque & common marmoset are alien
3 species, pigtail, stumptail & rhesus macaques, are indigenous
Those used for biomedical research are crab-eating & rhesus macaques
ncbi.nlm.nih.gov/books/NBK22179…
The following information on monkey breeding centres in China is from 2001.

There have been some changes...

For example, Hubei Topgene Biotechnology, the new supplier to WIV and WIBP was not listed 20 years ago. Image
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Tables in previous tweet are from

Chinese Primate Status and Primate Captive Breeding for Biomedical Research in China

ncbi.nlm.nih.gov/books/NBK22179…
From 2004, another table offers more information

Highlighted is Laboratory Animal Center of Chinese Academy of Military Medical Sciences in Beijing

From:

Notice of State Forestry Administration on Strengthening the Management of Experimental Monkeys

archive.ph/fIzFxImage
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To be continued..Homework

China has become the Mecca for those deluded Chinese & Foreign Researchers keen to abuse monkeys on the altar of Science.

While author Xin Hao gloats over low labor costs

Monkey Research in China
Developing a Natural Resource

cell.com/fulltext/S0092…Image
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Please Note

This thread is an investigation of the breeding centres, laboratory research evidence, logistics and locations of possible Biological Warfare research in China using non human primates.

It may lead nowhere or it might lead somewhere, that remains to be determined. Image
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More from @BillyBostickson

Aug 22
1. Nord Stream Update

3 years for truth to emerge

@MerlynThomas on 27/09/22 for @BBCWorld

Ukraine accuses Russia of pipeline terror attack

bbc.com/news/world-eur…

@JamesCrisp6 on 28/09/22 for @Telegraph

Why Putin would want to blow up Nord Stream 2

archive.ph/JCtYB x.com/Panchenko_X/st…
2. Hilariously

Merlyn Thomas has been working for the "BBC disinformation unit" since September 2021.

He certainly earned his UK STATE propaganda pay check with Nord Stream!

3. Invitation to venture down a Baltic rabbit hole!

You are hereby invited to read my 682 tweet thread investigating all angles of the Nord Stream sabotage, which ends here:



Thanks @Erkperk @VasilijProzorov @JeffreyBrodsky5 @MichaKobs @bopanc @liscer72
Read 4 tweets
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets

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