The vax is ~4200bp.
This is approximately 1 error in every vax molecule and you get injected with 40T.
This is a result of m1pU which is a low fidelity base. This low fidelity also impacts the next step of tRNA hybridization in translation.
This is compounding error.
Pfizer has mostly sold this base replacement as improving the magnitude and durability of expression.
As with many things in biology, when you optimize for the magnitude of expression, you sacrifice fidelity.
Many think of variants as from the virus, not the vax
The virus has an ExoN gene to error correct the polymerase errors. These error correcting proteins don’t like low fidelity bases like m1pU
In order maximize expression of m1pU they need sloppy enzymes
Add the transcriptional and translational compounded error and you have a combinatorial biochemistry problem on every injection.
This is why there is raw sequence data for vax lots in NCBI.
There is no peptide sequence of one of these mRNA libraries in NCBI.
Just smears on gels.
This is a highly variable prodrug.
The final drug has never been characterized.
There are 3D structures of protein translated from mRNA transcribed from a DNA construct without m1pU. Not relevant. No m1pU.
There are also smeary antibody stained western blots.
Antibody stains are biased toward error free proteins. They don’t tag the mutated ones.
Need to stain everything.
Cell free in vitro TNT to understand glycosylation or PNGase F for removing in vivo glycosylation. More ?s.
In summary, no mRNA injection should ever escape lot to lot sequence QC that is sensitive enough to find parts per thousand error. 40T molecules injected means small % error = billions of contaminants.
Protein sequencing of the final drug should be required.
This is why there is NO lot to lot raw sequence data in NCBI.^^^^
To simplify, the error measured by Chen et al, suggests 1 error in every mRNA molecule. Poisson would imply some molecules have 2 and 3 errors and many have zero.
Now imagine you inject 40 Trillion molecules where each one is different.
The combinatorics are mind blowing.
The good news is that folk at BASE are starting to look at this problem with direct RNA sequencing with Oxford Nanopore (ONT). But the m1pUs look foreign to the ONT platform and get called as both a C and a T. It's unlikely this will have the accuracy to pick up 1:1K heteroplasmy
The bad news is, this should have been done and made public before injecting 1B people. Its pretty bleeding edge so I can see how it was overlooked but at the minimum ILMN sequencing could have measured the heteroplasmies. Probably need UMIs to discount the cDNA syn error.
But now that the camel has its nose in the tent and has feasted on the money machine.. It will be back for more.
For those doubting my assessment of the mfg process, BASE spells it out here. Maybe my purity expectations are too high but I'd be anlot less scrupulous if Pfizer/Moderna put any raw sequence live for lot to lot QC. Zero for the vax. Millions for the virus.
This preprint monitors the translation fidelity in PseudoU and m1PseudoU.
If anyone has spare cycles, it would be helpful to calculate the Amino Acid edit distance of The vax Amino Acids to human amino acids in all 6 reading frames. This would give us a framework of the autoimmune risk of these synthesis errors.
The EMA leak has a lot to say about RNA integrity loss during manufacturing scale up.
If I had to guess,
Nucleotide purity probably played a role. It’s hard to scale up the synthesis of modified nucleotides and the polymerases don’t like to incorporate them. They stall.
Particularly at long tracks of U.
PolyU incorporation it pure N1 methyl pseudoU could create polymerase stall points and truncated mRNA.
But RNA integrity numbers also include mRNAs that are too long so one can’t assume it’s only truncated mRNA.
UPDATE!
More people have weighed in on this including the authors of the Re-adenylation paper.
They have been very transparent and helpful.
The plasmid DNA is there.
The 3’UTR sequence that matches the Fauci/Moderna synthetic constructs is shared sequence between the vaccines and points to a hole in our original assembly of the Moderna vaccines.
Here is how we know.
Thanks to @P_J_Buckhaults for suggesting this.
If you map reads to the Moderna HIV constructs, you only get coverage over the ends of their HIV vaccines (I checked 4).
You don’t get sequence coverage over the whole construct.
That implies there are shared parts of the plasmids in these Moderna vaccines.
Why does our Moderna vaccine have a 60bp hole in it? We sequenced a bivalent vaccine. The assemblers, when faced with 2 conclusions average then into a consensus and this 60bp is jumbled as a result.
BLAST is currently favoring the alignment to HIV vaccines over our Moderna C19 reference as it’s derived from monovalent sequence and more accurate.
🔥Another Contaminant Found in the Moderna Vaccines.
Did you consent to getting Moderna's HIV vaccine?
Parts of it are in there.
The recent Re-Adenylation paper has excellent sequence of not only the m1273 vaccine after application to mice.. but it also has the plasmid DNA
In addition to this plasmid DNA sequence, we can see sequences that map to a Moderna patent for an HIV vaccine in development.
As if the plasmid contamination isn't insulting enough, this envelope glycoprotein sequence has no business being in these data.
You can Thank Mitch McConnell for this Farm Bill trainwreck.
Legalize hemp as long as its NOT Delta 9-THC
Chemists say hold my beer.... or joint.
Suck it you chemically illiterate congressmen.
Here's Delta 8 THC, now in gas stations, smoke shops and online with limited age checks.
Plant doesn't make Delta8 THC so its not cannabis!
Now they have banking, no safety testing, interstate commerce, limited taxes and licensing nonsense. Im all for free markets but why is the natural safety tested Delta9 THC unbanked and federally illegal?
420 is a day to consider safe cannabinoids and their impact on autism.
We ordered 30 THCA Hemp flowers from online vendors that had minimal age checks.
What did we find?
56% were contaminated with Aspergillus Fumigatus.
@RobertKennedyJr @DrJBhattacharya @MartyMakary
Only 1 sample was below 0.3% THC hemp limit but even that sample was ~20% THCA which will become THC once burned.
4 samples were Lewis acid conversions of CBDA into delta8 THC.
Likely hemp sprayed with synthetics. Delta 8 is psychoactive. Less studied.
Evades the Hemp definition. But that Lewis acid also modified many other compounds in the plant into god knows what.