Another Pfizer funded study finds Pavlovid is wonderful and Jha just laps it up like a good Pharma pawn.
The he adds in another preprint with pharma ‘honorarium’ or bribe money.
He then links to a retrospective study that only looks at vaxxed people.

How many Retrospective IVM studies did he dismiss because they were retrospective?
Another preprint he fawns over demonstrates the older unvaxxed comorbid cohort was unvaxxed and did better with Pavlovid.

You don’t say.
Such obvious Confounders should be ignored by Faucists.

• • •

Missing some Tweet in this thread? You can try to force a refresh
 

Keep Current with Kevin McKernan 🙂

Kevin McKernan 🙂 Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

More from @Kevin_McKernan

Aug 27
Once you mix your 5 minute transposon step, another SPRI step is needed to kill the transposon enzyme and then a motor protein is added. This is unique to ONT sequencing. This pooled library just needs to quantitated on a Tape Station to get the concentration b4 loading. Image
The Flongles want 3-20 femptomoles of DNA.

They are tiny. 30uls are loaded. Image
Once you load this DNA, 10,000 reads in 30 minutes and 300,000 overnight.
These are single molecule reads that require GPUs for basecalling. Image
Read 6 tweets
Aug 27
Validating PCR assays has never been easier. This is why there is no excuse for clinical primers sets used in Covid to go to market without sequence data.
This is a $5K Oxford Nanopore Sequencer.
The flow cell (flongle) is $90 and is a real time sequencer. Image
In this study we are amplifying with two different ITS primers sets.
One set is 450bp in length and doesn’t amplify the host ITS region (Cannabis).
The other 750bp product captures plants and fungi.
ITS regions are from rDNA and organize the tree of life

biorxiv.org/content/10.110… Image
These are the key regions used to classify the taxonomy or eukaryotes. Bacteria use 16S regions and viruses have no such conserved regions to organize around.

Amplifying and sequencing these regions is the most common way microbiome studies are performed. Image
Read 8 tweets
Aug 14
Franklins Tower
Read 4 tweets
Aug 10
Just a reminder.
Hotez continues to flamed journals with hatred toward vex skeptics that require military intervention.
Choice words to discriminate against people that question his holy wisdom.
Read 5 tweets
Aug 5
Colonies from a cannabis flower.
What are they?

Toothpick into an 8 minute boil prep and we can PCR the ITS regions in an 1.5hours.

15minute TN5 transposon based rapid barcoding kit and we have DNA ready for Oxford Nanopore.
Agilent tape station used to quantify the PCR bands prior to TN5 transposition of the DNA barcodes.
230K reads in a few hours on a $5K instrument.

Sanger is dead.
Read 4 tweets

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!

Ethereum

0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy

Bitcoin

3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us on Twitter!

:(