Jeff Gilchrist Profile picture
Dec 10, 2022 27 tweets 9 min read Read on X
Toilets, wastewater COVID-19, RSV, and Influenza all increasing

This thread will be wastewater related, looking at new variants, levels, and what happens in the air after you flush that wastewater down the toilet with some cool video/graphics. 🧵1/ Image of aerosol plumes over a flushed toilet.
The wastewater levels in Ottawa, Canada are increasing for all measures ( ). RSV and Influenza A levels continue to increase which means even more pressure will be put on the hospitals that are already overwhelmed. 2/ covid.gilchrist.ca/Ottawa.html
Graphs showing increasing RSV and Influenza A wastewater levels in Ottawa, Canada
To make matters worse, COVID-19 levels have started increasing again. You can see in the graph that levels never actually returned to low levels but are on the rise again ( ). 3/ covid.gilchrist.ca/Ottawa.html
Graphs showing increasing COVID-19 wastewater levels in Ottawa, Canada
What might be making the difference now? New Omicron BQ based variants have rapidly been increasing in Ontario and as of 10 days ago already made up more than 40% of sequenced cases ( ). Graphs by @Mike_Honey_ 4/ app.powerbi.com/view?r=eyJrIjo…
Graph showing increasing levels of BQ.* variants in Ontario
The BQ.1 Typhon and BQ.1.1 Cerberus variants specifically have overtaken all the other circulating variants in Ontario and are much more immune evasive than previous variants. 5/ Graph showing increasing levels of BQ.1 and BQ.1.1 variants now dominant in Ontario
If wastewater levels are going up again it could be because more people are susceptible to this newer variant and is being more easily transmitted amongst people, even those that have been previously infected. 6/
Speaking of wastewater, a very cool study used lasers to actually see and measure the aerosol plumes that come out of toilets when you flush ( ). H/T: @yaneerbaryam 7/ nature.com/articles/s4159…
Image of aerosol plumes over a flushed toilet.
Aerosols can transmit infectious diseases including COVID-19, influenza and likely most respiratory viruses and even Strep bacteria has airborne spread ( ). H/T: @ELHopkins 8/thelancet.com/journals/lanmi…
When you flush a toilet, it releases plumes of tiny water aerosols into the air around you which can spread pathogens from human waste and expose people in public washrooms to contagious disease ( ). 9/ theconversation.com/toilets-spew-i…
Image of aerosol plume over a flushed toilet.
A typical commercial toilet generates a strong upward jet of air with velocities exceeding 2m/sec rapidly carrying particles up to 1.5m (5 feet) above the bowl within 8 seconds of the start of the flush which you can see in this video ( ). 10/
Toilet bowl water contaminated by feces can have pathogen concentrations that persist after dozens of flushes but it is still an open question as to how much of a transmission risk toilet aerosol plumes present. 11/
Particle size matters, the smaller aerosols can stay floating in the air for sometimes hours. Particles smaller than 5 microns can be breathed directly into your lower respiratory tract and those 6-12 microns can deposit in the upper airways ( ). 12/thelancet.com/journals/lanre…
The study measured a massive increase in particles smaller than 2.5 microns with most particles being less than 1 micron. 13/
Over the bowl (Loc 1 and 2 in diagram) particle counts increased more than 10x pre-flush rates and decreased afterwards, while 1.5m away from the bowl the particle count increase was lower but remained constant after flushing ( ). 14/ nature.com/articles/s4159…
Charts of aerosol particle size measurements from various locations.
While the visible aerosols in the video (5-10 microns) scatter more light from the lasers, they also captured the dimmer light scattered by smaller aerosols which found they move within the same envelope as the larger ones ( ). 15/ nature.com/articles/s4159…
Image showing small aerosol particles staying within plume with larger droplets fall quickly outside of plume.
Most interesting is they show large droplets follow ballistic trajectories, remain outside the aerosol plume envelope and drop quickly. Yet another blow to droplet theory being a significant contributor to transmission <darn laws of physics>. 16/
Closing the lid before flushing helps but does not completely eliminate plumes as there are still large gaps of space between the lid and the bowl when closed and many commercial toilets do not have lids at all. 17/
The researchers suggest that UV disinfection systems and better ventilation could help mitigate exposure to aerosol plumes in the bathroom. The next time you visit a public washroom, think of all the aerosolized human waste you are breathing in if you aren't wearing a mask... 18/
A lot of people have been saying to close the toilet lid first which doesn't stop the aerosols coming out and contaminates the underside of the lid that people touch as pointed out by this comment ( ). 19/
As @rnaguru points out, each pathogen is different, and so far studies haven't found much infectious COVID-19 particles in urine or feces so while the risk is not zero it may be very low. This may not be the case for other viruses/pathogens though ( ). 20/
But you will still want to wear your mask in public bathrooms to at least protect yourself from respiratory aerosols from the other people using the bathroom who may be infected (COVID, RSV, Influenza, etc...). 21/
It is not just breathing in aerosols released from flushing toilets that people need to worry about, but bacteria that gets deposited on your hands from hot-air hand dryers ( ). 22/ cnbc.com/2022/12/19/how…
Image showing person drying their hands using a hot air dryer in a bathroom
A study before the pandemic found that test plates exposed to bathroom air for 2 minutes with hand dryers off averaged < 1 bacteria colony while plates exposed to hand dryer air for 30 seconds averaged 18-60 colonies ( ). 23/journals.asm.org/doi/10.1128/AE…
When researchers swabbed the interior hand dryer nozzle, they found surfaces had minimal bacterial levels so they added HEPA filters to the hand dryers which reduced bacterial deposition by 4x. 24/
This implies the bacteria was coming from the bathroom air so just after washing your hands you get pathogens deposited on them right after again. 25/
Health experts recommended using paper towels to dry their hands instead and remind everyone that acquiring infection from direct contact with other people is much more likely than from drying your hands. 26/
This study found during contact tracing that someone got infected after using a shared bathroom 40 minutes after the index case left. A good example of it isn't just the people currently there in a public bathroom you need to worry about ( … ). 27/icjournal.org/DOIx.php?id=10…

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More from @jeffgilchrist

Nov 10
COVID-19: Stats, Guides, Articles, Calculators

COVID-19 guides, useful info, stats, calculators & 600+ scientific articles: covid.gilchrist.ca

Ottawa, Canada weekly stats: covid.gilchrist.ca/Ottawa.html

Ontario, Canada weekly stats: covid.gilchrist.ca/Ontario.html

🧵1/ Graph of COVID-19 wastewater levels in Ottawa, Ontario, measured from Sept. 1, 2021. Weekly stats available at ( http://covid.gilchrist.ca/Ottawa.html )
Sankey graph (height of each bar is # of sequences for that variant) showing top variants and their lineages over the past 6 weeks from PCR test genomic sequencing in Ontario, Canada. Visualization tool was created by @Mike_Honey_ ( https://app.powerbi.com/view?r=eyJrIjoiNzE5YzczODItMDQzMS00M2EzLWFjNWYtMjg3OTY3NTNhZDM3IiwidCI6ImRjMWYwNGY1LWMxZTUtNDQyOS1hODEyLTU3OTNiZTQ1YmY5ZCIsImMiOjEwfQ%3D%3D&pageName=ReportSection32240d6b62ba73667e98 )
A sample of some threads listed on the website ( covid.gilchrist.ca ):

How do variant designations work and relate to our immunity ( x.com/jeffgilchrist/… )? 2/
Why should we care about a virus with a "99.8% survival rate" ( )? 3/
Read 16 tweets
Nov 10
#Variant update for #Ontario, #Canada (to Oct 29, 2024)

The KP.3.1.1 #DeFLuQE variant is having some competition from XEC and MC.1 now. Graph tools by @Mike_Honey_ 🧵1/ Graph of top 7 COVID variants from PCR test genomic sequencing in Ontario, Canada. Visualization tool was created by @Mike_Honey_ ( https://app.powerbi.com/view?r=eyJrIjoiNzE5YzczODItMDQzMS00M2EzLWFjNWYtMjg3OTY3NTNhZDM3IiwidCI6ImRjMWYwNGY1LWMxZTUtNDQyOS1hODEyLTU3OTNiZTQ1YmY5ZCIsImMiOjEwfQ%3D%3D )
XEC is a combination of KP.3.3 and KS.1.1 that recombined together. MC.1 is a direct descendant of KP.3.1.1 with one spike mutation at T571I. To learn more about how variant naming works, you can read this article I wrote with @paulseaman31 ( ). 2/docs.google.com/document/d/1q0…
This Sankey graph shows the proportion of variants from PCR test genomic sequencing in Ontario since September 15, 2024. You can follow the lineage backwards to see where a variant is a descendant from. 3/ Sankey graph (height of each bar is # of sequences for that variant) showing top variants and their lineages over the past 6 weeks from PCR test genomic sequencing in Ontario, Canada. Visualization tool was created by @Mike_Honey_ ( https://app.powerbi.com/view?r=eyJrIjoiNzE5YzczODItMDQzMS00M2EzLWFjNWYtMjg3OTY3NTNhZDM3IiwidCI6ImRjMWYwNGY1LWMxZTUtNDQyOS1hODEyLTU3OTNiZTQ1YmY5ZCIsImMiOjEwfQ%3D%3D&pageName=ReportSection32240d6b62ba73667e98 )
Read 9 tweets
Sep 22
Ceilings fans can reduce short-range viral concentrations close to the infected (54%-77%) while increasing long-range levels (5%-14%). Only recommended when # people in room is less than decision tool for various ventilation rates with not highly contagious pathogens (e.g. cold).

Decision tool for using or not using fans to mix the air in the room. Decision tool for using or not using fans under various ventilation rates (200, 400, and 800 m³/h) on reducing overall airborne transmission risk, considering occupants with and without the use of masks. The logarithmic scale on the left vertical axis shows the contagiousness of the pathogen, which indicates the quanta emission rate. The horizontal axis measures the number of people at long-range distances in relation to per person ventilation rates. The range of conditions that using fans is beneficial is filled in turqu...
The number concentration of lumped tracer particles between 0.3 and 5.0 μm for both short-range and long-range routes, respectively.
Link to full study here ( ).sciencedirect.com/science/articl…
There are some important caveats in this research. "Fans are beneficial in well-ventilated rooms with not highly contagious pathogens." 3/
Read 4 tweets
Sep 19
Variants: JN.1 vs KP.2 vs KP.3.1.1

What do the variant designations like KP.3.1.1 mean and how does it compare to JN.1? How important is just 1 mutation? Can 1 mutation potentially affect our immunity and how vaccines are designed? Read on to find out more. @paulseaman31 🧵1/ Structural alignment of the AF2-predicted RBD conformational ensembles in the complexes with ACE2 for JN.1 (A), KP.2 (B) and KP.3 variants (C). Structural alignment of the AF2-predicted conformations with high pLDDT values > 80.0 and the cryo-EM structure of the BA.2.86 RBD-ACE2 complex (pdb id 8QSQ). The RBD conformations are shown in ribbons. The sites of unique BA.2.86 mutations D339H, K356T, R403K, V445H, G446S, N450D, L452W, N460K, N481K, A484K, F486P, R493Q are shown in blue spheres on panels (A-C). Mutation L455S in JN.1 is shown in red spheres on panel A. The positions of KP.2 mutat...
The web version of this article with nice table of contents and is easy to share with others can be found here ( ) or ( ). 2/ tinyurl.com/VariantsSummer…
docs.google.com/document/d/1q0…
Table of Contents Variant Designations (e.g. KP.3.1.1) - Parent & Child Lineages - KP.3 vs KP.3.1 vs KP.3.1.1 - Variants that Start with X How does this relate to our immunity? How important is 1 mutation? What variants are circulating now? - Canada - USA - United Kingdom - Europe - Asia - South America -Oceania
Variant Designations (e.g. KP.3.1.1)

You have probably seen people refer to COVID-19 variants with specific codes containing both letters and numbers like JN.1 or XBB.1.5, but what does that mean? 3/
Read 89 tweets
Aug 28
Virus update for Ottawa, Canada (Aug 28, 2024)

COVID-19 #Wastewater levels in #Ottawa are up again with the circulation of a new KP.3.1.1 DeFLuQE variant ( ). Wastewater is still being updated but on a weekly basis now. 🧵1/3 covid.gilchrist.ca/Ottawa.html
Image
New hospitalizations in Ottawa *due* to COVID-19, Flu or RSV are unfortunately increasing again. There were two big spikes over the past month ( ). 2/3 ottawapublichealth.ca/en/reports-res…
Graph of New Hospitalizations in Ottawa *due to* COVID-19, Influenza or RSV. Weekly stats available at ( http://covid.gilchrist.ca/Ottawa.html )
One virus is causing a lot more hospitalizations than the other two over the past year. Even at the lowest point at the end of March 2024, COVID-19 still accounted for more than 40% of hospitalizations between the three viruses and more than 80% for almost half the year. 3/3 Graph of % of New Hospitalizations in Ottawa *due to* COVID-19, Influenza or RSV. Weekly stats available at ( http://covid.gilchrist.ca/Ottawa.html )
Read 4 tweets
Aug 23
#Variant update for #Ontario, #Canada (to Aug 13, 2024)

Today's variant show is brought to you by the letters KP... Looks like KP.3.1.1 has really taken off and separated from the pack at 40% prevalence in Ontario. Graph tools by @Mike_Honey_ 🧵1/ Graph of top 7 COVID variants from PCR test genomic sequencing in Ontario, Canada. Visualization tool was created by @Mike_Honey_ ( https://app.powerbi.com/view?r=eyJrIjoiNzE5YzczODItMDQzMS00M2EzLWFjNWYtMjg3OTY3NTNhZDM3IiwidCI6ImRjMWYwNGY1LWMxZTUtNDQyOS1hODEyLTU3OTNiZTQ1YmY5ZCIsImMiOjEwfQ%3D%3D )
KP.3.1.1 has the #FLuQE mutations and also a key point in the spike protein deleted which gives it much more immune evasion than other variants. 2/
Testing with a recently released monoclonal antibody treatment (pemivibart) found that while it neutralized JN.1 and KP.2 with similar performance... ( ). 3/biorxiv.org/content/10.110…
Read 14 tweets

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