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Dr. Elinne Becket - @bielleogy on all platforms Profile picture
@bielleogy
Mar 1, 2023 • 40 tweets • 13 min read • Read on X
Of course I should have known my audience and known a group of microbiologists wouldn't let it slide without photographic evidence.
Promising culprit:
I'm never forgiving @ATinyGreenCell for this
My mom (who made the soup for my birthday) came across this thread and now 1) I have to answer for letting her soup spoil and 2) she's worried @ATinyGreenCell will figure out her secret recipe
THREE TIMES. Photo of a Tupperware with ...
Metagenomic DNA acquired! Photo of a nanodrop reading...
Blue beef soup swab, stab in beef bullion agar, and metagenomic DNA on its way to @ATinyGreenCell !! Photo of a gloved hand hold...
Trying to grow some myself in the mean time using beef bullion agar that smells like Top Ramen 😅 #BlueSoup Photo of an agar stab tube ...
Just thinking how absolutely ridiculous the Materials & Methods and Acknowledgements sections are going to be if #BlueSoup becomes a paper 🤣
No turning back now!
Lollll think #BlueSoup is a contender? 😆
Growth after 24h. There's some trace growth on the plate incubated at 4°C but hard to see from photo Photo of 3 bacterial agar p...
CALM DOWN EVERYONE @ATinyGreenCell and I have a plan
Since this is of great debate, no there is not cabbage, beets, garlic (surprising given my family uses it in everything), tomatoes, or soy milk in this recipe.

There's definitely at least 1 carrot though.
Alright we've figured out how the recipe will appear in the #BlueSoup paper 🤣
I forgot we had more witnesses to the genesis!
Left the plates where they were for another 2 days, except the 37°C one was brought to RT, which then grew white stuff over the yellow stuff and stinks to high heaven. RT looked the same. 4°C had impressive growth. Restreaked them all onto TECH agar, awaiting results! Photo of 3 agar plates side...
Since I'm catching flak for this, to be clear, these were literally just the blue swabs directly onto the plates to try to grow as much as I can while @ATinyGreenCell receives and revives the swabs I sent him, not an attempt to properly streak out samples.
No blue yet on TECH plates at any temps tested. @ATinyGreenCell is still gonna streak out for single colonies to see if he can find any blue ones hidden.
Dual-coast effort begins!
Observational evidence building...
Getting our Materials and Methods in order
Blue spectrum preliminary update! Absorbance peak @ 590nm with swab resusp in H2O and spun down. Gotta check w/ diff cuvette but very cool!
And the plated swab on differential media! Glows on King's Agar B but not A!
Iridescent colonies!
The isolates be cominggggg
This isolation progress continues
FEAR NOT, EVERYONE! The chemists are on it! #BlueSoup Photo of a gloved hand hold...
Blue soup to blue cheese....
#BlueSoup shotgun library! Gonna QC the size distribution tomorrow after I get some rest and fresh soup 🤧 Hopefully first thing next week it can be sent off to @seqcoast! Photo of a blue gloved hand...Photo of a DeNovix DS-11 fl...
Nothing yet on #ScienceCheese but it certainly smells unique!
#BlueSoup enzymatic testing Pt 1!
OK EVERYONE KEEP CALM #bluesoup
I HAVE IMPORTANT INFORMATION! 4°C versus RT!! Also no blue on LB + beef bouillon.

(Before anyone snarks that's an intentional heavy restreak of the original swab blob to keep as much as I could before Seb tested his swab - blue part is heavy part of streak! Density-dep??) Photo of two bacterial King...
The isolation quest continues:
Musing time:
Closer look at blue/indigo diffuse... Seems density dependent.
Back in the lab finally, and #BlueSoup sequencing library is good to go! Photo of a 100bp DNA gel la...
*Preliminary* #BlueSoup 16S-seq results:
Shotgun #BlueSoup data are back from @seqcoast! Prelim analyses support the 16S data: it's predominantly a mix of Pseudomonads, & we're diving in. However, despite using the domestic q-tip and crude trash can containment methods, there's only 0.08% homo sapiens DNA in there 🤣

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More from @bielleogy

Dr. Elinne Becket - @bielleogy on all platforms Profile picture
Mar 1, 2023
Our friend made the assertion that In-n-Out is overrated. So as scientists on a stormy day, we needed to test this properly (with options w/in our neighborhood).

Let me say up front we regretted this almost as soon as we started. 1/ Photo of 8 fast food/fast c...
Several have asked if we did this blind. For the most part, if one has had any of these, there's no way to hide what is what (especially for fellow Silicon Valley fans). 2/ Photo of eight quarters of ...
And now.... ::drumroll::... the results! (rank order) 3/ Photo of 4 people's rank or...
Read 7 tweets
Dr. Elinne Becket - @bielleogy on all platforms Profile picture
Mar 1, 2023
Ok I'm outing myself here but there was forgotten beef soup in our fridge we just cleaned it out and it was BLUE?!?!? Wtf contam would make it blue??? Like BRIGHT blue!! 🤢🤮 Even w/ all my years in micro I'm not handling this well.
I can't believe y'all talked me into going back into the trash. Photo of rotten beef soup t...
Of course I should have known my audience and known a group of microbiologists wouldn't let it slide without photographic evidence.
Read 23 tweets
Dr. Elinne Becket - @bielleogy on all platforms Profile picture
Oct 27, 2022
Things I didn't know until after my first external grant as a PI, that I wish I had known before submitting:

- What are indirects, how much are they, and to whom do they go to, and for what

- Who "manages" your grant, orders, hiring, etc, and the processes for each

1/3
- How wages/stipends are disbursed, who can be paid off them, what are the restrictions, and what are the processes

- How progress reports happen and what support do you (not) get

- How ordering happens, for different cost levels, & when it has to go through procurement

2/3
- How much time I'd spend managing my grant instead of doing the proposed work

Those are off the top of my head, but if anyone has something to add that can benefit ECRs, drop them here 👇

(And yes I knew what indirects were but I didn't REALLY know what indirects were)

3/3
Read 4 tweets

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