1. Alternatives to Gain of Function Research
Loss of Function Research
Molecular dynamical modeling of viral proteins
Interactions with inhibitors and receptor
In vitro studies of specific properties required for human adaptation using single proteins
archive.ph/Lzwxz
2. More Alternatives

In vitro studies of genetic interactions between loci in viral proteins using replication-incompetent viruses – epistatic interactions

Sequence database comparisons of genetic properties of human and animal adapted viruses

thebulletin.org/2022/06/gain-o…
3. Ethical Alternatives

"to Experiments with Novel Potential Pandemic Pathogens"

journals.plos.org/plosmedicine/a…
4. The Seven Deadly Sins of....

Biomedical Research

by Laura H. Kahn

gjia.georgetown.edu/2023/03/03/the…Image
5. The latest egregious example in a long list

6. Now is the time to support @BiosafetyNow



@BioSRP
@pricklyresearch
@R_H_Ebright
@mattwridley
@gdemaneuf
@WashburneAlex
@Ayjchan
@alisonannyoung
@BillyBostickson
@nicholsonbaker8
@Bryce_Nickels
@tony_vandongen
@angoffinet
@BiophysicsFL
@ColinDavdButler biosafetynow.orgImage
7. What Needs To Be Made Public

All proposals & progress reports discussing dangerous virus experiments & documents related to the planning of the experiments

Details as to when, where & why such experiments were planned including any review processes involved

Risks & Benefits Image
8. Question of Biowarfare Research

It is now clear (to me) that the US, despite its denials, has pursued an illegal biowarfare program, by outsourcing collection of deadly viruses around the world & making them more virulent & pathogenetic.

We now have all the evidence we need
9. Public Denials, Ongoing Research.

Of course, The US Government and the Scientists involved will never state that they are engaged in biowarfare research, but rather "biodefense" and the noble drive to develop vaccines and prophylactics for deadly viruses & bacterial diseases. Image
10. The New Mantra: "Protecting the War Fighter"

Bio-Defense Now:

56 Suggestions for Immediate Improvements Final Report 85% Biological Defense Project (2005)

US Air Force Counterproliferation Center

airuniversity.af.edu/Portals/10/CSD…Image
11. Recently Highlighted in the #DEFUSE Documents

US Defense Advanced Research Projects Agency (DARPA) program to protect the US “warfighter”
by reverse engineering dangerous SARS-like bat viruses and creating aerosol vaccine sprays for use in bat caves

drasticresearch.org/2021/09/20/158…Image
12. No Need for Resurrection

of more controversial elements of US cold war biowarfare projects (well documented), e.g. aerosolisation of pathogens & their respective delivery programs, as they know exactly how to do all this

Now there is a wider repertoire of offensive weapons Image
Image
13. Extent of Pentagon Biowarfare Program?

Pentagon linked researchers like Lipkin (Mailman) Nichol (Atlanta) Baric (UNC) Bavari, Totura (USAMRIID), Denison, Munster (Rocky Mountain) & DARPA, DTRA, PREDICT Scientists involved could tell us a great deal!

14. Final Point in This Thread

We cannot only point the finger of blame at the US

Russia has continued Soviet work on biological weapons, but I will discuss that in a new thread



Did the Russian program encourage the Pentagon to covertly pursue its own?thebulletin.org/2022/10/the-ru…
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More from @BillyBostickson

Sep 11
1. "We’ll be seen as spies, not scientists.”

x.com/emilyakopp/sta…

Daszak "seems" confused when questioned BY @RepJamesComer whether he ever worked with, or for, spooks.

Unsurprisingly in light of what is shown on page 79 of the DRASTIC EHA files

researchgate.net/publication/39… x.com/Dylan_Housman/…Image
2. He may claim it was post pandemic contact

But thanks to @AGHuff we know it went back quite a while, at least to 2015.

@AGHuff 3. More questions from @NameIsSpartacus

We need to find out why Peter Daszak & EcoHealth had In-Q-Tel backing and why Nathan Wolfe (an obvious intelligence asset with links to Epstein/Maxwell, Boris Nikolic, and Bill Gates) was on their editorial board.

Read 14 tweets
Sep 10
Good to see Generation Z in Asia growing a set of balls and fighting back against shit governments.



#NepalGenZProtest #Myanmar #SriLanka

Hopefully #China will be next!
Just 3 years ago #SrilankaProtest

One year ago #BangladeshProtest

Read 10 tweets
Sep 6
🧵My comments and some conclusions regarding the EHA documents we found:

1. Peter Daszak actively schemed to foil congressional and other investigations into EcoHealth Alliance (EHA) and its ties to the Wuhan Institute of Virology, as revealed in the DRASTIC document stash.
2⃣ He orchestrated a “Coalition of Support” with former USAID officials like Dennis Carroll and NGOs like the Smithsonian, which sent letters (e.g., April 24, 2020, to NIH) decrying grant cuts as political attacks to deflect scrutiny (Part 6, p.35-36).
3⃣ Daszak hired the Kyle House Group (KHG) to lobby against NDAA defunding amendments, with KHG’s Porter joining meetings like one on August 4, 2023, with Rep. Adam Smith’s staff to kill Sen. Joni Ernst’s provisions (Part 7, p.52).
Read 16 tweets
Aug 22
1. Nord Stream Update

3 years for truth to emerge

@MerlynThomas on 27/09/22 for @BBCWorld

Ukraine accuses Russia of pipeline terror attack

bbc.com/news/world-eur…

@JamesCrisp6 on 28/09/22 for @Telegraph

Why Putin would want to blow up Nord Stream 2

archive.ph/JCtYB x.com/Panchenko_X/st…
2. Hilariously

Merlyn Thomas has been working for the "BBC disinformation unit" since September 2021.

He certainly earned his UK STATE propaganda pay check with Nord Stream!

3. Invitation to venture down a Baltic rabbit hole!

You are hereby invited to read my 682 tweet thread investigating all angles of the Nord Stream sabotage, which ends here:



Thanks @Erkperk @VasilijProzorov @JeffreyBrodsky5 @MichaKobs @bopanc @liscer72
Read 4 tweets
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets

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