Thread by @_HeartofGrace_

1/ VIRAL REACTIVATION: The SV40 Promoter on DNA Plasmids found in mRNA "Vaccines":
The study below does not mention the SV40 promoter, at all.

The SV40 promoter can possibly interact with intra-condensates and influence viral reactivation.
(purple circles=plasmids)

https://twitter.com/renesugar/status/1656795962071986181

2/Intra-condensates are dynamic liquid-like compartments inside the nucleus, which are involved in gene regulation, playing an integral role in gene expression.
The SV40 promoter contains DNA sequences that are binding sites for

3/ transcription factors and some other regulatory proteins. These factors and proteins can interact with intra-condensates, which can affect the accessibility and activity of the SV40 promoter.

4/ The interaction between the SV40 promoter and intra-condensates may influence viral reactivation. The formation and behavior of intra-condensates can act on the SV40 promoter and the recruitment of factors and co-regulators needed for viral gene expression.

5/ IF a DNA PLASMID entered the nucleus of a cell, changes may occur in nuclear organization and
the interactions of the SV40 promoter and intra-condensates could influence viral reactivation.

6/ Sources:

Chong et al. (2018) Intra-nuclear concentration of membrane-associated proteins determines the outcome of herpesviral infection

Strom et al. (2017) - Phase separation drives heterochromatin domain formation

7/ Amin-Wetzel et al. (2020) The chromatin-associated protein Dpr1 is a repressive regulator of RNA polymerase I transcription

Altmeyer et al. (2015) Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

#plasmidgate

@CharlesRixey
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