Thread by @_HeartofGrace_

1/👀 OH! ANTIBODIES CLASS SWTICHING? IS the SV40 PROMOTER to BLAME?
The SV40 promoter, which is part of the starter plasmid that McKernan discovered in vials of Pf!zer mRNA "vaccin3s", can bind to many different things--like several classes of proteins.

thailandmedical.news/news/covid-19-…!

2/ Scientists have used that SV40 promoter to instigate the expression of certain factors involved in class switching ANTIBODIES, to elucidate the underlying processes. By forcing the SV40 promoter into B cells or cell lines, researchers have

3/ studied their impact on class switching.
Researchers have used SV40 promoter to drive the expression of activation-induced cytidine deaminase (AID), the enzyme directly involved in class switching. By using the SV40 promoter, researchers have

4/ forced class switching to different antibody isotypes. the SV40 promoter has been used by researchers in studies to force the expression of other transcription factors, co-factors, or regulatory elements implicated in class switching (antibodies)

5/ sources:
Sen, R., & Kastner, P. (1993). Enhancer interference and immunoglobulin heavy chain class switch recombination. Trends in Biochemical Sciences, 18(5), 176-180. doi: 10.1016/0968-0004(93)90161-t

--analysis of studies that utilize the SV40 promoter in expression

6/ Chaudhuri, J., et al. (2007). Evolution of the immunoglobulin heavy chain class switch recombination mechanism. Advances in Immunology, 94, 157-214. doi: 10.1016/s0065-2776(06)94006-3 : use of SV40 promoter

7/ Sale, J. E., et al. (2001). The molecular mechanism of class switch recombination: Balancing long-range synapsis and exonuclease processing. Genes & Development, 15(23), 3266-3277. doi: 10.1101/gad.943001

Immunoglobulin class switch and SV40 promoter

#plasmidgate