Christie Laura Grace Profile picture
May 12 7 tweets 2 min read Twitter logo Read on Twitter
1/👀 OH! ANTIBODIES CLASS SWTICHING? IS the SV40 PROMOTER to BLAME?
The SV40 promoter, which is part of the starter plasmid that McKernan discovered in vials of Pf!zer mRNA "vaccin3s", can bind to many different things--like several classes of proteins.

thailandmedical.news/news/covid-19-…! Image
2/ Scientists have used that SV40 promoter to instigate the expression of certain factors involved in class switching ANTIBODIES, to elucidate the underlying processes. By forcing the SV40 promoter into B cells or cell lines, researchers have
3/ studied their impact on class switching.
Researchers have used SV40 promoter to drive the expression of activation-induced cytidine deaminase (AID), the enzyme directly involved in class switching. By using the SV40 promoter, researchers have
4/ forced class switching to different antibody isotypes. the SV40 promoter has been used by researchers in studies to force the expression of other transcription factors, co-factors, or regulatory elements implicated in class switching (antibodies)
5/ sources:
Sen, R., & Kastner, P. (1993). Enhancer interference and immunoglobulin heavy chain class switch recombination. Trends in Biochemical Sciences, 18(5), 176-180. doi: 10.1016/0968-0004(93)90161-t

--analysis of studies that utilize the SV40 promoter in expression
6/ Chaudhuri, J., et al. (2007). Evolution of the immunoglobulin heavy chain class switch recombination mechanism. Advances in Immunology, 94, 157-214. doi: 10.1016/s0065-2776(06)94006-3 : use of SV40 promoter
7/ Sale, J. E., et al. (2001). The molecular mechanism of class switch recombination: Balancing long-range synapsis and exonuclease processing. Genes & Development, 15(23), 3266-3277. doi: 10.1101/gad.943001

Immunoglobulin class switch and SV40 promoter

#plasmidgate

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More from @_HeartofGrace_

May 11
1/ VIRAL REACTIVATION: The SV40 Promoter on DNA Plasmids found in mRNA "Vaccines":
The study below does not mention the SV40 promoter, at all.

The SV40 promoter can possibly interact with intra-condensates and influence viral reactivation.
(purple circles=plasmids) Image
2/Intra-condensates are dynamic liquid-like compartments inside the nucleus, which are involved in gene regulation, playing an integral role in gene expression.
The SV40 promoter contains DNA sequences that are binding sites for
3/ transcription factors and some other regulatory proteins. These factors and proteins can interact with intra-condensates, which can affect the accessibility and activity of the SV40 promoter.
Read 8 tweets
May 11
👀Re; mRNA v@ccines: Science/med people: I took the entire PLASMID gene sequence from here:

anandamide.substack.com/p/sequencing-t…

placing it into an epitope bioinformatics epitope/antigen detector, and it spit out this:
iedb.org/result_v3.php?…
Don't let me have all the fun!

Thoughts?
Image
Read 4 tweets
May 10
DNA plasmids can interact with SOME DEAD BOX (DDX#) proteins in the human body. It would be especially concerning if an mRNA based "vax": contained plasmids that housed a known cancer causing SV40 promoter. Image
Turbo Cancer: Pfizer mRNA Vaccines, DNA Contamination, the SV40 Promoter, and DEAD-Box Proteins

christielauragrace.substack.com/p/turbo-cancer… Image
Read 7 tweets
Apr 20
1/ 🧵A negative net charge on a Lipid Nanoparticle can cause adverse events, including: allergic reaction, cytokine storm, off target expression of immunogens, clots, and amplifying the frequency and severity of ALL adverse events. Pfizer calculated the net charge on the LNP
2/ in their initial studies to be approximately - 3 mV.
However, this should have been calculated without the presence of any DNA plasmids, only lipids (cholesterol, PEG, ionizable lipids, and helper lipids (DSPC)) and RNA with positive and negative charges (cation lipids/RNA). ImageImageImage
3/ Today I asked ChatGPT a series of questions about lipid nanoparticles and DNA plasmids. At first it said there were no plasmids present in the LNP within the Pf!zer vaccine product, which @Kevin_McKernan has proven to be false. anandamide.substack.com/p/sequencing-t…
Read 15 tweets
Apr 20
Hmmm, AI, RNA, DNA plasmids, and LNPs.
I have some questions for it.

Here is one of the first I just asked it:
"What is the zeta potential of a pfizer lipid nanoparticle for the covid 19 vaccines which also contains DNA plasmids?"
Wrong answer. Image
Now it is being cheeky with me.
(I am J and I gave it what it wanted--size, composition resting in human blood with pH accordingly, and I named the plasmids.) Image
Read 7 tweets
Apr 20
I've got an interview tomorrow, which I'll be introducing what happens when you combine RNA, plasmid DNA, and cationic ionizable lipid with LNP including static charges and zeta potential--the lipid nanoparticle LIPOPLEX hybrid.
It's going to get interesting.
@Kevin_McKernan
@Kevin_McKernan Hey
@Kevin_McKernan
, what do you suppose would happen if not only LNP formed with RNA, but LIPOPLEX formed in hybrid, secondary and tertiary structures formed, and due to electrostatic forces, the cationic lipids formed lipoplexes with the DNA plasmid?
I'll explain it tomorrow. Image
@Kevin_McKernan Hey @Kevin_McKernan serious question I hope you can answer. If not off the top of your head I will go after the sequences in your substack. Is there a transcription factor sequence embedded in that plasmid? I remember off the top of my head potential for nuclear transport. Ty
Read 4 tweets

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