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The TRILOGY that changes the face of the pandemic :
VIROPORIN, ION CHANNELS, MUTATION T9I in the E PROTEIN
VIROPORIN, ION CHANNELS, MUTATION T9I in the E PROTEIN
2) We recently covered these 3 topics, once again. First of all, viroporins.
https://twitter.com/ejustin46/status/1705251426182525244?t=IOXk39O84plagNO8dpxydQ&s=19
3) Then very recently the ion channels and the key role of the envelope protein.
https://twitter.com/ejustin46/status/1705572818711093661?t=MgaWzmr6x3NuaH1fQeCHsw&s=19
4) Concerning the E:T9I mutation, we have addressed this subject several times, showing that it has changed the game with the Omicrons, and then with the mutation E:T11A for the XBBs.
https://twitter.com/ejustin46/status/1648182241770500098?t=w3_hodYF25MEUL6k89_CZw&s=19
5) We would like to return to this trilogy of viroporin, ion channels and E:T9I mutation, with a study that we will not publish (to avoid unnecessary reactions) but from which we will quote extracts.
"The envelope protein of SARS-CoV-2 (2-E) forms a homopentameric cation channel
"The envelope protein of SARS-CoV-2 (2-E) forms a homopentameric cation channel
6) ...that is important for viral pathogenicity. Our previous study demonstrated that the E- protein alone is sufficient to induce cell death, provoke a cytokine storm, and even cause acute respiratory distress syndrome-like damage in vivo."
7) "The allele frequency of the mutation T9I (threonine to isoleucine) in all statistical samples was higher than 99.49%."
"T9I in the envelope protein of the SARS-CoV-2 Omicron variant changes channel properties and attenuates virus production and virulence"
"T9I in the envelope protein of the SARS-CoV-2 Omicron variant changes channel properties and attenuates virus production and virulence"
8) "The results revealed that the ion selectivity of T9I mutant channels is different from that of WT channels." (Fig.1C and D)
"Compared with exogenous expression of the WT protein, exogenous expression of the T9I mutant significantly attenuated the viral load (Fig. 1H).
"Compared with exogenous expression of the WT protein, exogenous expression of the T9I mutant significantly attenuated the viral load (Fig. 1H).
9) "As shown in Figure 1I, in comparison with that of WT, the cytotoxic ability of T9I was approximately 150-fold lower, as was cytokine production."
To sum up, "the reduced potassium efflux and additional chloride influx mediated by T9I channels ...
To sum up, "the reduced potassium efflux and additional chloride influx mediated by T9I channels ...
10) ...cause less severe ionic dyshomeostasis, membrane disruption, and lysosome-related cell death."
This doesn't mean that Omicron is less dangerous but its pathogenicity is very different from the previous VOC.
This doesn't mean that Omicron is less dangerous but its pathogenicity is very different from the previous VOC.
11) The almost exclusive discourse on Spike and immune escape mutations, the virtual refusal to take into account the changes in pathogenicity with the E:T9I mutation in Omicrons and E:T11A in XBBs, the failure to take into account the absence of the E:T11A mutation ...
12) ... of the XBB in BA.2.86, which brings its pathogenicity to the level of the first Omicrons, all this has not facilitated a clear discourse on a pandemic, which has changed its face.
13) Thanks for reading 🙏
FYI
@siamosolocani @SystemsVirology @shay_fleishon @mrmickme @DavidJoffe64 @arijitchakrav @HarrySpoelstra @C_A_Gustave @outbreakupdates @GourlaySyd @_ppmv @AltenbergLee @UseBy2022 @RolandBakerIII
FYI
@siamosolocani @SystemsVirology @shay_fleishon @mrmickme @DavidJoffe64 @arijitchakrav @HarrySpoelstra @C_A_Gustave @outbreakupdates @GourlaySyd @_ppmv @AltenbergLee @UseBy2022 @RolandBakerIII
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