1/PURE ZETA: PART TEN:
Thanks to the recent discovery of DNA contamination by Dr. Phillip Buckhaults, Kevin Mckernan and others, Dr. David Speicher, Dr. Rose finding DNA vs batch vs adverse event, Dr. Sebine (PEG) and others--this proves so much, including change in zeta.
2/ This series provides a background on lipids, their activities, charges, colloids, the human body, how to determine the charge on a lipid nanoparticle, what influences it, where it goes in the body, and how it starts the cascade for adverse events. openvaers.com
3/ If it were not for the very hard work of those listed above, and so many behind the scenes, people would not be able to combine the work of others towards their own findings. Many people have faced harsh consequences as a result of doing this work at much personal risk. TY
4/ Zeta potential is not a new concept--not a hypothesis. This is known, and has been known, for years--far before the idea of the LNP ever became the sparkle in the eye of some scientist in a lab somewhere. It is the charge on a particle that determines what it does.
5/ This is all a summary and a repeat of what has been said in other threads. Pf!zer et al., determined the zeta potential, in a bulk solvent (that does not represent the human body in its entirety) to have a zeta potential, of approximately -3 mV.
"To avoid binding events"
6/ We have covered what a binding event means": binding with other molecules, ions, proteins, other lipids, the endothelium, platelet factor 4--there are many things the LNP can bind with if the charge swings positive or negative. But it can also bind due to "hydro" interactions
7/ This can cause clots, immune cascade, cytokine storm, binding with the endothelium to express the protein in the capillary walls, microclots, and kick off, the rapid hypercoagulation cascade that is proven to result form negatively charged liposomes interacting with the blood.
8/ The thick clots people are saying do not exist, were proven in a study by Fazulin et al, early 2020. They finished this study in 2019. It was published in 2020. t proves highly negatively charged liposomes drive clot formation by 1000s of times that of what usually occurs.
9/ when zeta potential was measured, it was measured using a bulk solvent, with process ONE, this is when the modRNA was made by using IVT enzymes, which do not employ E coli, or plasmids. DNA to RNA to protein--IVT enzymes, to RNA to protein. The zeta was measured by pf!zer
10/ by measuring the LNP, after it was made, using process ONE--without the presence of any plasmids or E coli. It was an entirely separate manufacturing process for the clinical trials. Process TWO, the stuff being injected into everyone's arms, used DNA plasmids to make RNA.
11/ Pf1zer states very clearly, in their own document, that they measured the LNP zeta potential to be closer to neutral, to "avoid non specific binding events in the blood compartment". THEY KNEW. They knew that if this would swing positive or negative, this would be bad.
12/ Multiple studies have proven, that if the LNP is not neutral, that if you change the RATIO of charges inside the LNP, to be more positive, or more negative, it changes WHERE the NLP goes, and what it does. If it is neutral, it goes to liver. If Positive, it goes to lung.
13/ Slightly negative, goes to spleen. And VERY negatively charged LNP, will leak right into the vascular. This does not matter if you inject into muscle or vein. It does not matter if you aspirate. That talk needs to stop right now.
14/ So, the original zeta potential was measured in a bulk solvent. But when it was measured, there was no E coli contamination or endotoxin from using plasmids, and there was no DNA contamination, therefore, the measured zeta is INCORRECT. Pf1zer got it wrong.
15/ The DNA plasmids have a high negative charge, they are very electronegative. This means any introduction, ANY, would cause the zeta potential to shift to be even MORE negative, causing it to want to bind with things, bind with the endothelium, and leak into the vascular.
16/Speicher and McKernan (TY!) recently found certain batches /lots of Pf!zer had higher contamination than others, and they linked these up to certain batches, and lo and behold, the higher the DNA content, the more adverse events! (myocarditis!) anandamide.substack.com/p/world-counse…
17/ If you look at those batch numbers on that chart, the more DNA in the batch, the higher number of people who got hurt. And if you go to how bad is my batch. com and you type in those lot numbers, you will see many events of myocarditis. knollfrank.github.io/HowBadIsMyBatc…
18/ This proves that the zeta potential is shifting negative, and starting the cascade of adverse events. It is NOT being caused by some "bolus theory". Just stop. I was told that they are entertaining that because that guy is a friend. That is not science. That is nepotism.
19/ The change in the PEg, which creates a protective barrier on the outside of the LNP, is not uniform, and has also been found, that when looking at specific batches, those batches with LESS peg, had more adverse events! This also proves that zeta is playing an important role.
20/ The work of others in Germany, as well as Dr G Pain finding endotoxin in batches, which also, has a negative charge, which comes from the E coli, from which the plasmids are "grown up" is also shifting the zeta even further negative, causing the start of the adverse events.
21/ Kids died. They killed kids. Of course, but someone's son and daughter is now deformed, or dead. Every part of this platform has a concern. It is not ready for mass scale. This platform was used prior, for only people who were suffering. That was their plan A.
22/ Plan B was RNA with LNP, but it was certainly never made with E coli and junk materials. it still carries risk. That is why, this has only been used for small cohorts, of maybe 50 people, in clinical trials, who do not have long to live, because they have genetic diseases.
23/ But the presence of the DNA plasmids confirm the zeta potential is shifting negative, and the work of Speicher, McKernan and Rose, proves this by their findings that the lots with higher DNA plasmid contamination have more adverse events.
24/ This is not a hypothesis, it is not a theory, and quite frankly, it is not up for debate. Anyone saying otherwise is trying to sell you something.
1/ 🚨 dsDNA [PLASMID CONTAMINATION IN LNP/modRNA 💉] CAN CAUSE STROKES
BREAKING STUDY PUBLISHED 10/17/2023
(possible part one--might need more threads)
THE STUDY: "Novel insight into cGAS-STING pathway in ischemic stroke: from pre- to post-disease" doi.org/10.3389/fimmu.…
2/Study highlights (layman's terms coming!)
cGAS-STING pathway is mediator of inflammation in response to dsDNA.
The cGAS-STING pathway is linked with start of the neuroinflammatory response in ischemic stroke.
The cGAS-STING pathway is involved in various forms of RCD.
3/ Breakdown for "X" (brief explanation of steps):
The presence of dsDNA activates the cGAS-STING pathway--part of the innate immune system. The cGAS-STING pathway detects dsDNA, which kicks off the immune and inflammation response, which leads to ischemic stroke.
1/ LNP: LIPIDS and RAPID CANCER: Part 1: deep dive
We've al been discussing DNA plasmids, and reports of rapid/sudden onset of cancers.
Contamination can't be the only baddie.
The lipids must be playing a role in this, by altering the mononuclear phagocyte system.
2/ The mononuclear phagocyte system involved in immune surveillance, which is the process of identifying and eliminating cancerous cells. Macrophages and dendritic cells can detect aberrant cells and present tumor antigens to T cells, initiating an immune response against
3/the cancer. This immune surveillance can help prevent cancer development and progression.
Tumor-Associated Macrophages (TAMs) are part of cancer growth and spread, and part of the MPS.
They can also be co-opted by tumors to promote their growth and spread. Tumor cells can
1/ PURE ZETA: PART NINE:
EXPLAIN IT TO ME LIKE I AM 12 VERSION--TIME FOR SCIENCE MATH!
MANY of you can learn this!
Zeta shifting is driving the START of many adverse events. The equations look complicated, BUT, if you break down each section, this is "plug and play" algebra.
2/ Here is a basic LNP. These numbers and symbols are arbitrary for teaching purposes only. We are leaving out the PEG, Cholesterol, and DSPC--only focusing on charges. They will be inaccurate in numbers--this is just to learn the way that you add them--a visual representation.
3/ One component to this calculation is determining the NET charge. Zeta potential is the surface charge in relation to all other things around the LNP. You have the ions in your body, the "thickness" of the tissue or blood (viscosity), molecules, pH, and a few other things.
1 PURE ZETA: PART EIGHT:
EXPLAIN IT TO ME LIKE I AM 12 VERSION:
(2 more threads to wrap it all up)
This is complex stuff---if you are not a scientist and you are understanding these threads, you are ahead of the game, because I talk to doctors and scientists who do not get it.
2/ Imagine an LNP as a speedboat traveling along a river. The speedboat represents the lipid nanoparticle, and the river symbolizes the bloodstream or other place in the body where the LNP is traveling. It is stealth most likely, due to the PEG, hiding it from the immune system.
3/The boat has a magnetic force around it, like an invisible magnetic bubble. This magnetic force is similar to the zeta potential of the LNP.
The boat's magnetic force can be strong or weak, just like the zeta potential can be highly negative or less negative (or even positive).
PURE ZETA: PART SEVEN: LNP: How Zeta Changes in the Human Body (layman's), and Why Pharma Companies are Incorrect Stating their Calculation of Zeta Potential (surface charge) is Accurate, much less, that it will not do harm.
Zeta is the starting point for most adverse events.
2/ (read previous threads for full explanations on LNP/Colloids/lipids/Zeta)
Zeta is calculated by physical means, and also equations (Smoluchowski/Henry), as previously explained in these threads, in depth (for X). Zeta is the kickoff point to adverse events. It is the ignition.
3/ Fact: the measured zeta that drug companies said would not bind, -3.13 mV, was measured in a bulk solvent, and had NOTHING to do with the human body. DNA plasmid contamination, changes in PEG, both correlated to specific batches in adverse events, PROVEs this (next thread).
1/ PURE ZETA: PART SIX
BASIC CHARGES: THE PREQUEL
Almost ALL biochemical reactions in the body involve movement of charged particles or the participation of charged molecules in some way.
This is a huge topic being ignored that is the driver behind almost all adverse events.
2/ There are positive (+) charges and negative (-) charges. A positive charge results from an excess of positively (+) charged particles--protons (+), within an object. A negative (-) charge is from an excess of negatively (-) charged particles--electrons, within an object.
3/ Positively charged particles are called "cations". They have more positive charges in them compared to negative. Negatively charged particles are called "anions". Charges can be be altered based on interactions with other objects (like a balloon!)