Thread by @_HeartofGrace_

1/ 🚨 OMG!
Introducing foreign DNA Plasmid into THE DEVELOPING BRAINS
of mice can lead to
SEIZURES, DAMAGE in brain development, such as consequences on microglia behavior, which could cause brain abnormalities, various cognitive disorders, cerebral palsy, neuronal damage!

2/ The study: 2018
Neocortical Microglia Express Toll-Like Receptor 9 and Respond to Plasmid DNA Injected into the Ventricle: Technical Considerations Regarding Microglial Distribution in Electroporated Brain Walls
eneuro.org/content/5/6/EN…

3/ These researchers raise concerns about the unintended effects of introducing genetic material into the developing brain, specially PLASMID DNA In humans, it could suggest that when manipulating genes or introducing foreign genetic material during early brain development,

4/ there may be unintended consequences on microglia behavior, which could impact brain development and function. It highlights the importance of carefully considering and understanding the immune response when performing genetic interventions in developing brains, especially

5/ in the context of neurodevelopmental studies or potential gene therapies for neurological disorders.

This study looked at the role of microglia, immune cells in the developing brain, and their distribution during embryonic development. It explains that when plasmid DNA is

6/ injected into the brain ventricle, microglia unexpectedly aggregate on the surface of the cortex and in the choroid plexus due to the activation of a specific immune receptor called Toll-like receptor 9 (TLR9).
What this means: Microglia are a type of immune cell in the

7/ developing brain (and adult brains--it is present in all ages), and how they are spread out during the early stages of brain development. It goes on to say that when scientists injected a certain type of genetic material (PLASMID DNA) into the brain, these microglia

8/ cells clumped together on the outer surface of the brain and in another part called the choroid plexus. This clumping happened because of the activation of a special immune receptor in these microglia cells called Toll-like receptor 9 (TLR9).
This can cause serious

9/ harm to a baby's brain when developing, if we consider that throughout science, we use mice as a model for what we might expect in humans.
TLR9 is a part of the immune system that recognizes certain types of genetic material, particularly those found in DNA PLASMIDS.

10/ When TLR9 is activated, it triggers an immune response, which, in this case, caused the microglia to aggregate. This response might be a protective mechanism in the brain to address the introduction of foreign genetic material, such as the injected plasmid DNA.

11/ 🚨AMOUNTS!!!
"We showed that exposure to as little as 25 pg of intraventricular LPS (a smaller amount than that contained in plasmid DNA solutions purified with the QIAGEN plasmid Maxi Kit) could attract microglia toward the apical surface. Importantly, although ODN 2088

12/ coadministration coupled with endotoxin-free plasmid DNAs restored microglial aberrant distribution, it did not completely inhibit microglial aggregation in the choroid plexus, indicating that other molecular mechanisms might function for sensing plasmid DNAs.

13/ 🚨omg! When DNA plasmid is introduced into the brain of a developing baby mouse, cytokines like TNF-α and IL-6 WILL ACT on neural stem/progenitor cells and the differentiation of precursor cells into various cell types within the brain. These cytokines can influence

14/ cell proliferation, differentiation, and survival, potentially modifying the brain's physiological environment.
Tumor Necrosis Factor-alpha (TNF-α) is a proinflammatory cytokine. In the brain, it can influence the rate at which neural stem and progenitor cells divide and

15/ produce new cells. If there's an abnormal increase in TNF-α levels, it causes excessive proliferation, resulting in an overproduction of cells, which might disrupt the normal structure and function of the developing brain of a baby in the womb.
TNF-α can also affect how

16/ precursor cells specialize into different types of brain cells, such as neurons, astrocytes, or oligodendrocytes. Disruptions in this process may lead to an imbalance of cell types, affecting the development of neural circuits and brain connectivity.
This means

17/ 🚨🚨The specialization of precursor cells into different cell types is not well-regulated and balanced, it can potentially lead to problems in how the brain is wired and functions. This may result in cognitive and neurological issues or developmental disorders.

18/ TNF-α can influence the survival of neural cells. An excess of TNF increases rate of cell death (apoptosis. This could result in an inadequate number of cells in certain brain regions, potentially leading to structural abnormalities and functional deficits.

19/ 🚨🚨This can Neonatal Brain Injury, causing apoptosis of developing neurons. This can cause structural abnormalities and functional deficits in the affected brain regions, potentially resulting in conditions like cerebral palsy or intellectual disabilities.

20/ 🚨😮Autoimmune Encephalitis via DNA PLASMIDS. This cytokine can contribute to the destruction of neural cells, leading to a range of neurological and psychiatric symptoms, including seizures, cognitive deficits, and behavioral disturbances. IN BABIES.

21/ 🚨🚨TNF-α and other inflammatory cytokines may play a role in mood disorders like depression. An imbalance in TNF-α levels could contribute to the loss of neurons in certain brain regions, impacting mood regulation and potentially leading to depressive symptoms. IN KIDS!

22/ DNA PLASMIDS CAUSE an excess of TNF which is harmful to neural cells in various disease states, leading to increased cell death and reduced cell survival. This can result in structural abnormalities in the brains of babies, and functional deficits, contributing to the

23/ development and progression of conditions like neurodegenerative diseases, neonatal brain injury, autoimmune encephalitis, and mood disorders.
DNA PLASMIDS CAN CAUSE THIS

@jathorpmfm
This is what you are seeing in your patients, correct?

@jordanbpeterson
@drdrew
@SenatorRennick
@P_McCulloughMD
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