Thread by @christlet

Lost in the StayGold monomeric variants? Here's a thread to try to sort things out 🧵
In 2022, the Katayama/Miyawaki labs published StayGold, a bright and stable fluorescent protein (FP) from jellyfish C. Uchidae: @NatureBiotechnature.com/articles/s4158…

The issue was that StayGold is dimeric and not easy to use as a fusion protein. Here's a link to a couple of plasmids from this article available @Addgene: and more plasmids from the Japanese NRBP: (not sure how available they are)addgene.org/browse/article…
rrc.nbrp.jp/references/705…

A preprint also recently assessed the photostability of StayGold using point-scanning confocal microscopy, spinning disk or widefield illumination: @biorxivpreprintbiorxiv.org/content/10.110…

In 2023, 3 articles reported monomeric variants of StayGold with a confusing mStayGold naming.
1) The Miyawaki lab first preprinted a variant with multiple point mutations : which was published a couple of days ago @naturemethodsresearchsquare.com/article/rs-294…
nature.com/articles/s4159…

This mutant is called mSG1 or QC2-6 FIQ in the article. @FPbase entry is here:
No plasmids are available on Addgene yet. There is also another mutant described in the article with a different linker, mSG2 or QC2-6(PT) with no obvious advantage over mSG1.fpbase.org/protein/qc2-6-…

2) The second preprint describing a monomeric StayGold is from the Mishima/Crow/Balasubramanian labs and was published yesterday: @NatureBiotechresearchsquare.com/article/rs-268…
nature.com/articles/s4158…

This is a single E138D mutation of the dimeric StayGold, hence the mStayGold E138D naming. The @FPbase page is here:
No Addgene plasmid available yet as far as I can see.fpbase.org/protein/mstayg…

3) This summer the Piatkevich/Subach labs preprinted a third variant they called… mStayGold, which has 8 mutations from StayGold and optimized N- and C-termini: @researchsquareresearchsquare.com/article/rs-318…

They compared its photostability to the second variant (called StayGold E138D in their preprint). Their variant has been renamed mBaoJin on @FPBase:
No final published version or Addgene plasmid available at this point I think.fpbase.org/protein/mbaoji…

Hope this is useful for everyone interested in these new FPs (@manorlaboratory @Fran_Bottanelli @joachimgoedhart @RetoPaul), and that I didn't made a mistake in this confusing but exciting blooming of mStayGold variants 👀