Thread by @BillyBostickson

1. Early 2020 #Pangolin #coronavirus & hACE2 Mice experiments have now led to disturbing results in Beijing (2024)!

Lethal Infection of Human ACE2-Transgenic Mice Caused by SARS-CoV-2-related Pangolin Coronavirus GX_P2V



h/t @outbreakupdates & @gadboit biorxiv.org/content/10.110…

https://twitter.com/BillyBostickson/status/1523385173240610816

2. Yang Ruifu: CCP's biological weapons expert



Note his earlier role in such experiments (Pango Covs & hACE2 Mice) h/t @coroldo1

in 2020, South China:

https://twitter.com/BillyBostickson/status/1315272494727614465

https://twitter.com/BillyBostickson/status/1393471781470556160

@coroldo1 3. Details from the New paper (Beijing)



"We previously* reported that the early passaged GX_P2V isolate was actually a cell culture adapted mutant, named GX_P2V (short_3UTR), which possesses a 104-nucleotide 21 deletion at the 3’-UTR"biorxiv.org/content/10.110…

@coroldo1 4, "Previously" refers to another paper co-authored by Yigang Tong in late 2022

Induction of significant neutralizing antibodies against SARS-CoV-2 by a highly attenuated pangolin coronavirus variant with a 104nt deletion at the 3'-UTR

ncbi.nlm.nih.gov/pmc/articles/P…

@coroldo1 5. Pan Coronavirus Vaccines?

GX_P2V has high genomic homology with SARS-CoV-2. The SP & RBD of GX_P2V share 92.4% & 86.8% amino acid identity with SARS-CoV-2 respectively.

The attenuating nature of GX_P2V(short_3UTR), led us to propose it as a potential live vaccine candidate

@coroldo1 6. Bact to the 2024 Paper

"In this study we cloned this mutant & assessed its pathogenicity in hACE2 mice. We found GX_P2V (short_3UTR) clone can infect hACE2 mice, with high viral loads detected in both lung & brain tissue & infection resulted in 100% mortality for hACE2 mice"

@coroldo1 7. More detail on Clones and Mutations

@coroldo1 8. A Warning!

"This is the first report showing that a SARS-CoV-2- 106 related pangolin coronavirus can cause 100% mortality in hACE2 mice, suggesting a risk for GX_P2V to spill over into humans."

biorxiv.org/content/10.110…

@coroldo1 9. WIV Research (2023)

"Our findings are inconsistent with those of Zhengli Shi et al. who tested virulence of GX_P2V in 2 different hACE2 mouse models"

SARS-CoV-2-Related Virus from Pangolin Causes Lung Infection without Severe Disease in hACE2 Mice

ncbi.nlm.nih.gov/pmc/articles/P…

@coroldo1 10. GX-P2V & GX-P2V (short 3UTR)

1. They did not isolate wild-type GX_P2V strain (Yigang Tong)

2. Sudy by ZLS et al. also tested GX_P2V (short_3UTR) variant but failed to reproduce their results,

3. They wish to study further adaptative evolutionary changes of this variant

@coroldo1 11. Gain of Function?

4. According to additional infection experiments, the uncloned GX_P2V(short_3UTR) also resulted in 100% mortality in hACE2 mice.

5. We cloned & analyzed mutations in GX_P2V (short_3UTR), focusing specifically on the pathogenicity of the cloned strains.

@coroldo1 12. More Gain of Function?

6. "The high pathogenicity mechanism of GX_P2V C7 in hACE2 mice, in the absence of the wild-type GX_P2V control, requires further investigation"

7. GX_P2V C7 has undergone a virulence enhancing mutation via spike protein mutations!

@coroldo1 13. The research has come a long way since 2020!

https://twitter.com/BillyBostickson/status/1393471781470556160

@coroldo1 14. Interesting find by @breakfast_dogs

A postgraduate recruitment document related to Yigang Tong's projects and GX-P2, linked in this tweet (forced download)



which in turn references a PLA major logistics "secret project" running from 2016 to 2019

https://twitter.com/breakfast_dogs/status/1744029204277301387

@coroldo1 @breakfast_dogs 15. The Postdoctorate Job

1. Optimization of coronavirus GX_P2V virus infection system.



Archived:



Found by @breakfast_dogs

scc.pku.edu.cn/attached/job-d…
archive.ph/gQGX5

https://twitter.com/breakfast_dogs/status/1744029209163657231

@coroldo1 @breakfast_dogs 16. Interesting Trio of Chinese CX P2 Researchers..

(Yigang Tong, Wuchun Cao, George Fu Gao)

Received March 2023, work done late 2022?

Mouse model for pangolin-origin coronavirus GX/P2V/2017 infection & cross-protection from COVID-19 subunit vaccine

sciencedirect.com/science/articl…

@coroldo1 @breakfast_dogs 17. If new to this topic or interested in delving deeper:

Massive thread on Professor Yigang Tong and GX P2 Coronavirus starts here:



Ends here:



Thread:



Archived:

https://twitter.com/BillyBostickson/status/1386623489138823168

https://twitter.com/BillyBostickson/status/1387531371464306690

threadreaderapp.com/thread/1386623…
archive.ph/zUKhX

@coroldo1 @breakfast_dogs 18. One of the more significant findings

https://twitter.com/BillyBostickson/status/1387130305354428417

19. Yigang Tong's Patent (applied Feb 8 2021)

的千金藤素抑制冠状病毒复制的倍数为15393倍。

"Stephanatine" inhibits coronavirus replication by 15,393 times



Archived



Patent Images



Translations Next Tweet xueqiu.com/1717074141/219…
archive.ph/79K0x
xueqiu.com/1717074141/219…

20. Translated Image from Patent ZL202110172158.7

(Google Translate)

Translated Post Doc Job Description

(Google Translate)

National Medical Products List (China)

(Google Translate)

21. The Patent discussed at length and in detail

"Recently very popular patent, ZL202110172158.7, invention patent for "Pangolin coronavirus xCoV and its application of drug anti-coronavirus infection", which attracted the attention of many people...."

archive.ph/d9v2N

22. "Stephanotis"

is an extract of common herb in China (family Fangbiaceae), also known as frog grass, ivy, golden-threaded turtle, male rat vine,

"It was discovered that Stephanotis has a great and irreplaceable effect in suppressing coronavirus"

inf.news/en/nature/6f4c…

23. As the Media pick up on this story, we see the complete bastardisation of community notes to the whims and lies of the virology community.

Example

https://twitter.com/BillyBostickson/status/1747613146645512210