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May 23 โ€ข 5 tweets โ€ข 1 min read โ€ข Read on X
๐—œ๐—ป๐—ณ๐—น๐˜‚๐—ฒ๐—ป๐˜‡๐—ฎ ๐—›5๐—ก1 ๐—ฎ๐—ป๐—ฑ ๐—›1๐—ก1 ๐˜ƒ๐—ถ๐—ฟ๐˜‚๐˜€๐—ฒ๐˜€ ๐—ฟ๐—ฒ๐—บ๐—ฎ๐—ถ๐—ป ๐—ถ๐—ป๐—ณ๐—ฒ๐—ฐ๐˜๐—ถ๐—ผ๐˜‚๐˜€ ๐—ถ๐—ป ๐˜‚๐—ป๐—ฝ๐—ฎ๐˜€๐˜๐—ฒ๐˜‚๐—ฟ๐—ถ๐˜‡๐—ฒ๐—ฑ ๐—บ๐—ถ๐—น๐—ธ ๐—ผ๐—ป ๐—บ๐—ถ๐—น๐—ธ๐—ถ๐—ป๐—ด ๐—บ๐—ฎ๐—ฐ๐—ต๐—ถ๐—ป๐—ฒ๐—ฟ๐˜† ๐˜€๐˜‚๐—ฟ๐—ณ๐—ฎ๐—ฐ๐—ฒ๐˜€ ๐Ÿ˜จ

H/t @DecrolyE @BrianRWasik
medrxiv.org/content/10.110โ€ฆ
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2) Highly pathogenic avian influenza H5N1 was detected in US dairy cattle herds in 2024, spreading between states and infecting at least one worker. Assessments found unpasteurized milk from infected cows contained high levels of infectious H5N1 virus.
3) To understand virus exposure risks to dairy workers during milking, the persistence of H5N1 and surrogate H1N1 influenza viruses were examined in unpasteurized milk on surfaces found in milking equipment like rubber inflation liners and stainless steel.
4) Experiments demonstrated that both H5N1 and H1N1 remained infectious in milk on these surfaces for hours under conditions mimicking milking parlors. This virus persistence poses risks for worker exposure through contaminated surfaces and spread between cattle during milking.
5) Reducing transmission requires protective equipment for workers as well as disinfection or replacement of liners to limit virus spread between animals.

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More from @ejustin46

May 25
๐—•๐—ฎ๐—ฐ๐˜๐—ฒ๐—ฟ๐—ถ๐—ฎ๐—น ๐—ฅ๐—ฒ๐˜€๐—ถ๐˜€๐˜๐—ฎ๐—ป๐—ฐ๐—ฒ ๐—”๐—บ๐—ผ๐—ป๐—ด ๐—œ๐—ป๐—ฝ๐—ฎ๐˜๐—ถ๐—ฒ๐—ป๐˜๐˜€ ๐—”๐—บ๐—ถ๐—ฑ๐˜€๐˜ ๐˜๐—ต๐—ฒ ๐—–๐—ข๐—ฉ๐—œ๐——-19 ๐—ฃ๐—ฎ๐—ป๐—ฑ๐—ฒ๐—บ๐—ถ๐—ฐ

๐˜ˆ ๐˜ฎ๐˜ฆ๐˜ต๐˜ข-๐˜ข๐˜ฏ๐˜ข๐˜ญ๐˜บ๐˜ด๐˜ช๐˜ด

This scoping review examined 55 studies evaluating changes in antibiotic resistance (AMR) ... sciencedirect.com/science/articlโ€ฆ
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2) ... among clinical cultures before and after the COVID-19 pandemic. Most common pathogens studied were Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus. Of 464 AMR results, 18% increased, 15% decreased ... Image
3) ...67% no change. Gram-negatives made up 69%, with 16% increased AMR, 15% decreased, and 69% no change. Gram-positives were 31%, with 24% increased, 14% decreased, 62% no change AMR. Image
Read 4 tweets
May 25
๐—ฆ๐—”๐—ฅ๐—ฆ-๐—–๐—ผ๐—ฉ-2 ๐˜ƒ๐—ถ๐—ฟ๐—ฎ๐—น ๐—ฑ๐˜†๐—ป๐—ฎ๐—บ๐—ถ๐—ฐ, ๐˜ƒ๐—ถ๐—ฟ๐—ฎ๐—น ๐—ฅ๐—ก๐—” ๐—ฎ๐—ป๐—ฑ ๐˜ƒ๐—ถ๐—ฟ๐—ฎ๐—น ๐˜๐—ถ๐˜๐—ฒ๐—ฟ

ascpt.onlinelibrary.wiley.com/doi/10.1002/psโ€ฆ
Image
2) A viral dynamic model was developed to characterize SARS-CoV-2 dynamics based on viral RNA and titer levels from 720 patients with COVID-19 infected with Omicron. The model used a target cell limited approach incorporating non-productive eclipse phase cells, productive cells Image
3) ...that produce infectious virus which can become non-infectious, and an immune response stimulated by productive cells. Model fitting estimated vaccination delayed time from infection to symptoms from 3.0 to 3.6 days and strengthened the immune effect 1.1-fold.
Read 4 tweets
May 25
๐—œ๐—ป๐˜๐—ฟ๐—ฎ๐—ป๐—ฎ๐˜€๐—ฎ๐—น ๐˜ƒ๐—ฎ๐—ฐ๐—ฐ๐—ถ๐—ป๐—ฎ๐˜๐—ถ๐—ผ๐—ป ๐˜„๐—ถ๐˜๐—ต ๐—ฎ๐—ป ๐—ก๐——๐—ฉ-๐˜ƒ๐—ฒ๐—ฐ๐˜๐—ผ๐—ฟ๐—ฒ๐—ฑ ๐—ฆ๐—”๐—ฅ๐—ฆ-๐—–๐—ผ๐—ฉ-2 ๐˜ƒ๐—ฎ๐—ฐ๐—ฐ๐—ถ๐—ป๐—ฒ ๐—ฝ๐—ฟ๐—ผ๐˜๐—ฒ๐—ฐ๐˜๐˜€ ๐—ฎ๐—ด๐—ฎ๐—ถ๐—ป๐˜€๐˜ ๐——๐—ฒ๐—น๐˜๐—ฎ ๐—ฎ๐—ป๐—ฑ ๐—ข๐—บ๐—ถ๐—ฐ๐—ฟ๐—ผ๐—ป ๐—ฐ๐—ต๐—ฎ๐—น๐—น๐—ฒ๐—ป๐—ด๐—ฒ๐˜€

๐˜•๐˜ฆ๐˜ธ ๐˜ด๐˜ต๐˜ถ๐˜ฅ๐˜บ !
nature.com/articles/s4154โ€ฆ
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2) Researchers developed a Newcastle disease virus (NDV)-vectored vaccine expressing the spike protein of SARS-CoV-2 in a pre-fusion stabilized conformation. This chimeric spike protein contains 6 proline substitutions to stabilize it in the pre-fusion state. Image
3) When delivered intranasally, this NDV-PFS vaccine (expressing the pre-fusion stabilized spike protein) induced robust immune responses in Syrian hamsters and K18 mice. Image
Read 9 tweets
May 25
๐˜ผ ๐™—๐™ง๐™ž๐™ก๐™ก๐™ž๐™–๐™ฃ๐™ฉ ๐™™๐™š๐™จ๐™˜๐™ง๐™ž๐™ฅ๐™ฉ๐™ž๐™ค๐™ฃ ๐™ค๐™› ๐™ฉ๐™๐™š ๐™ƒ5๐™‰1 ๐™จ๐™ž๐™ฉ๐™ช๐™–๐™ฉ๐™ž๐™ค๐™ฃ
๐™—๐™ฎ @DuckSwabber ๐™–๐™—๐™ค๐™ช๐™ฉ ๐™ฉ๐™๐™š ๐™จ๐™ฉ๐™ช๐™™๐™ฎ ๐™ค๐™› @keshu_VRimmCh

๐Ÿงต ๐˜ˆ๐˜ฏ ๐˜ฆ๐˜น๐˜ฑ๐˜ญ๐˜ข๐˜ฏ๐˜ข๐˜ต๐˜ช๐˜ฐ๐˜ฏ ๐˜ช๐˜ฏ ๐˜ญ๐˜ข๐˜บ๐˜ฎ๐˜ข๐˜ฏ ๐˜ต๐˜ฆ๐˜ณ๐˜ฎ๐˜ด Image
2) A "panzootic virus" is a term to describe a virus that spreads rapidly among animals of different species, causing a widespread outbreak. Unlike an epidemic that affects only one species, a panzootic virus can cross species barriers and infect a wide range of animals Image
3) "Glycosylation sites in HA" are specific locations where sugar molecules attach to the influenza virus protein. They shield the virus from the immune system, influence its stability, and affect infectivity. Variations in glycosylation patterns impact virus emergence. Image
Read 7 tweets
May 25
๐™-๐™˜๐™š๐™ก๐™ก ๐™š๐™ฅ๐™ž๐™ฉ๐™ค๐™ฅ๐™š๐™จ ๐™ž๐™ฃ ๐™Ž๐˜ผ๐™๐™Ž-๐˜พ๐™ค๐™‘-2 ๐™ซ๐™–๐™ง๐™ž๐™–๐™ฃ๐™ฉ๐™จ

(๐˜๐˜ช๐˜ณ๐˜ด๐˜ต ๐˜ด๐˜ต๐˜ถ๐˜ฅ๐˜บ ๐˜ต๐˜ฐ ๐˜ฆ๐˜น๐˜ฑ๐˜ญ๐˜ฐ๐˜ณ๐˜ฆ ๐˜ต๐˜ฉ๐˜ฆ ๐˜š๐˜ˆ๐˜™๐˜š-๐˜Š๐˜ฐ๐˜-2 ๐˜Š๐˜‹8+ ๐˜ฆ๐˜ฑ๐˜ช๐˜ต๐˜ฐ๐˜ฑ๐˜ฆ ๐˜ฅ๐˜ช๐˜ท๐˜ฆ๐˜ณ๐˜ด๐˜ช๐˜ต๐˜บ ๐Ÿ‘)
frontiersin.org/journals/immunโ€ฆ
Image
2) The study analyzed predicted CD8+ T cell epitopes in spike, membrane, and nucleocapsid proteins of 16 SARS-CoV-2 variants for 27 common HLA class I alleles in the US population. This covered over 90% of Caucasians and 65-75% of other ethnicities. Image
3) Spike epitopes were the least conserved between variants, while membrane and nucleocapsid epitopes were highly conserved. As the virus mutated, a greater proportion of spike epitopes showed reduced predicted binding affinity to HLA alleles. Image
Read 8 tweets
May 24
๐˜พ๐™ค๐™ฌโ€™๐™จ ๐™ˆ๐™ž๐™ก๐™  ๐˜พ๐™ค๐™ฃ๐™ฉ๐™–๐™ž๐™ฃ๐™ž๐™ฃ๐™œ ๐˜ผ๐™ซ๐™ž๐™–๐™ฃ ๐™„๐™ฃ๐™›๐™ก๐™ช๐™š๐™ฃ๐™ฏ๐™– ๐˜ผ(๐™ƒ5๐™‰1) ๐™‘๐™ž๐™ง๐™ช๐™จ
๐˜๐˜ฆ๐˜ข๐˜ต ๐˜๐˜ฏ๐˜ข๐˜ค๐˜ต๐˜ช๐˜ท๐˜ข๐˜ต๐˜ช๐˜ฐ๐˜ฏ ๐˜ข๐˜ฏ๐˜ฅ ๐˜๐˜ฏ๐˜ง๐˜ฆ๐˜ค๐˜ต๐˜ช๐˜ท๐˜ช๐˜ต๐˜บ ๐˜ช๐˜ฏ ๐˜”๐˜ช๐˜ค๐˜ฆ

๐˜ฟ๐™ค๐™ฃ'๐™ฉ ๐™™๐™ง๐™ž๐™ฃ๐™  ๐™ง๐™–๐™ฌ ๐™ข๐™ž๐™ก๐™  ! ๐Ÿ’ฅ
H/t @KrutikaKuppalli
nejm.org/doi/full/10.10โ€ฆ
Image
2) The authors report the detection of HPAI H5N1 virus in nasal swabs and milk of dairy cows. They isolated eight HPAI A(H5N1) viruses from milk samples and compared their genetic origin with other viruses. Image
3) ๐™ƒ๐™š๐™–๐™ฉ ๐™ž๐™ฃ๐™–๐™˜๐™ฉ๐™ž๐™ซ๐™–๐™ฉ๐™ž๐™ค๐™ฃ ๐™š๐™ญ๐™ฅ๐™š๐™ง๐™ž๐™ข๐™š๐™ฃ๐™ฉ๐™จ ๐™จ๐™๐™ค๐™ฌ๐™š๐™™ ๐™ฉ๐™๐™–๐™ฉ ๐™จ๐™ฅ๐™š๐™˜๐™ž๐™›๐™ž๐™˜ ๐™ฉ๐™š๐™ข๐™ฅ๐™š๐™ง๐™–๐™ฉ๐™ช๐™ง๐™š๐™จ ๐™–๐™ฃ๐™™ ๐™™๐™ช๐™ง๐™–๐™ฉ๐™ž๐™ค๐™ฃ๐™จ ๐™ง๐™š๐™™๐™ช๐™˜๐™š๐™™ ๐™ซ๐™ž๐™ง๐™ช๐™จ ๐™ฉ๐™ž๐™ฉ๐™š๐™ง๐™จ ๐™—๐™ช๐™ฉ ๐™™๐™ž๐™™ ๐™ฃ๐™ค๐™ฉ ๐™›๐™ช๐™ก๐™ก๐™ฎ ๐™ž๐™ฃ๐™–๐™˜๐™ฉ๐™ž๐™ซ๐™–๐™ฉ๐™š ๐™ฉ๐™๐™š ๐™ซ๐™ž๐™ง๐™ช๐™จ. Image
Read 5 tweets

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