7/ [dee2]() Digital Expression Explorer 2. Digital Expression Explorer 2 (DEE2) is a repository of uniformly processed RNA-seq data mined from public data obtained from NCBI Short Read Archive. By Ziemann Mark ! Version 2 of dee.dee2.io et.al
8/ Extracting allelic read counts from 250,000 human sequencing runs in Sequence Read Archive biorxiv.org/content/10.110…
9/ [MetaSRA: normalized sample-specific metadata for the Sequence Read Archive]()biorxiv.org/content/early/…
10/ [ARCHS4: Massive Mining of Publicly Available RNA-seq Data from Human and Mouse]() ARCHS4 provides access to gene counts from HiSeq 2000, HiSeq 2500 and NextSeq 500 platforms for human and mouse experiments from GEO and SRA.amp.pharm.mssm.edu/archs4/
11/ [DEP-reads: Uniformlly processed public RNA-Seq data]() Read counts data for 5,470 human and mouse datasets from ARCHS4 v6 and 12,670 datasets from DEE2 for 9 model organisms by steven Ge.bioinformatics.sdstate.edu/reads/
12/ [SRA-explorer]() This tool aims to make datasets within the Sequence Read Archive more accessible.ewels.github.io/sra-explorer/
13/ [intropolis]() is a list of exon-exon junctions found across **21,504** human RNA-seq samples on the Sequence Read Archive (SRA) from spliced read alignment to hg19 with Rail-RNA.github.com/nellore/introp…
14/ [batch recompute ~20,000 RNA-seq samples from larget sequencing project such as TCGA, TARGET and GETEX](). Used `hg38` and `gencode v21` as annotation.genome-cancer.soe.ucsc.edu/proj/site/xena…
15/ [A cloud-based workflow to quantify transcript-expression levels in public cancer compendia]() used kallisto for TCGA/CCLE datasets and gencode v24 as annotation.biorxiv.org/content/early/…
16/ [MiPanda]() is an online resource for the interrogation and visualization of gene expression data from the myriad of publicly available cancer and normal next generation sequencing datasets.mipanda.med.umich.edu
Are protein and RNA correlated? 12 papers and examples 👇 🧵 what do you think?
1/ It is gene-specific, see figure 2D from Quantitative Proteomics of the Cancer Cell Line Encyclopedia buff.ly/3PqSvSL
2/ [The relationship of mRNA with protein expression in CD8+ T cells associates with gene class and gene characteristics | PLOS ONE]() “Limited correlation of mRNA and protein abundance in CD8+ T cell populations”buff.ly/3Z9qNgy
10 tools/papers related to bulk-RNAseq deconvolution.👇 🧵 #computationalbiology #RNAseq Even in the era of single-cell RNAseq, bulk-RNAseq data are still very valuable.
1. [Benchmarking of cell type deconvolution pipelines for transcriptomics data]()buff.ly/458vpp3
2. [A benchmark for RNA-seq deconvolution analysis under dynamic testing environments]()buff.ly/3xdIEo6
1/ [CuratedAtlasQueryR]() is a query interface that allows the programmatic exploration and retrieval of the harmonized, curated and reannotated CELLxGENE single-cell human cell atlas.buff.ly/46DKAIv
2/ Hallmarks of transcriptional intratumour heterogeneity across a thousand tumours https://t.co/PcX6JOys1pbuff.ly/3PC5iCe buff.ly/3qWCOJ6