First…
They measure how long is spike expressed in cells.
7 days out it is higher than day 1. Peaks at day 5.
Using 3 different Fluorometry methods they zero in on DNA levels before and after RNaseA treatments.
4-5X over once RNA is removed.
The AccuBlue dyes had the least cross talk.
They dismantle the Kaiser paper over its use of EtOH precipitation
Most importantly they show the spike is packaged in exosomes which likely renders most ELISA assays used to track this somewhat blind to quantitation and begs the question of shedding since exosomes are exhaled and secreted in the skin.
@TGAgovau are you awake yet?
A very good discussion on SV40 and its reckless use given its known functionality.
Forward this paper to all politicians and regulators.
@FLSurgeonGen @DrJBhattacharya @MartyMakary @SenatorRennick @RobertKennedyJr @RWMaloneMD @BroadbentMP @P_McCulloughMD @MendenhallFirm @Double_Christ
The review actually started in July so sailing through is probably a misnomer.
And the cat photo is from nightcafe AI.
They still seem to have problems with hands and paws!
Just to connect the dots on the exosomes.
This would explain shedding and if any of these exosomes have replication competent plasmids, we have a mess on our hands.
The Beck et al work likely also has fecal/oral routes of transmission.
The Karen Konclave may start buying masks again:)
• • •
Missing some Tweet in this thread? You can try to
force a refresh
This is a dense read but it will help you to understand why the DNA fluorometer data is lighting up so much on modRNA. This is much more cross-talk than you see with traditional ssRNA.
That is because these are intercalating dyes that bind to minor grooves are only found in double stranded nucleic acids.
The fact that RNaseA is showing such a reduction in signal is because the RNA isnt single stranded.
At least half of it is digestable by a dsRNA specific nuclease known as RNaseIII.
Another portion responds to RNaseH which only digests RNA in RNA:DNA hybrids.
The fraction that responds to RNAseIII should worry you. Your cells have RNaseIII and its the start of the RNA interference pathway.
To make matters worse, Pharma is using an ELISA that is blind to dsRNA <400bp. It cant see this problem. And they know it exists
Your response to peoples request for your qPCR protocol is misinformation.
No transparency on your methods but we can already see that you are using only 1 assay in the vector (Kan gene) so you are under estimating the load 100X.
You should know this by now as Speicher et al published this a year ago.
@TGAgovau This is misinformation. Dr. David Speicher's latest test used RNaseA. There is no RNA interfering with the fluorometry.
Did you not even read the report?
Life must be good at the TGA when you can be a year behind all the time.
@TGAgovau Part of GMP and ISO is having your protocol open for inspection.
Is SV40 Large Tumor Antigen required for SV40 origin of replication activity.
No
Here we detect Pfizer plasmid DNA in a colon tumor biopsy 1 year after vaccination.
And its not small amounts of DNA. Its so much DNA that it can only be explained by plasmid amplification post vaccination or genome integration and amplification.
Variants are found in the SV40 Promoter that do not exist when you sequence the vaccine directly suggestive of replication errors once transfected into mammalian cells.