Thread by @dbdugger

I will always regard Harry as a disreputable degenerate for failing to consider my lived patient experience as I have advocated for my family members of the past three years. I'm not the only one who has drawn parallels to HIV, there is Akiko Iwasaki.

https://twitter.com/alanbarnard1/status/1962111268107489764

Where might the vascular remodeling be taking place? What about in the lungs? It is evidence of Pulmonary Artery Hypertension developing that provided the first evidence of Nef-like mediated damage.

2021 Jan 7
Evidence of Pulmonary Hypertension after SARS-CoV-2 Infection in Subjects without Previous Significant Cardiovascular Pathology
pmc.ncbi.nlm.nih.gov/articles/PMC78…

Ten months later, the protein that heavily contributes to its development appeared here.
SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis
nature.com/articles/s4146…

"20. Schwartz, O., Maréchal, V., Le Gall, S., Lemonnier, F. & Heard, J. M. Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein. Nat. Med 2, 338–342 (1996)."

It was also in 2021 that we learned of Tat-like mediated damage through Corrected QT Interval Prolongation developing.
COVID-19 Infection and Corrected QT Interval Prolongation—Collateral Damage From Our Newest Enemy
jamanetwork.com/journals/jaman…

Not observed in ME/CFS and that it does not present in that population has only been known for nineteen years.
Short QT interval
me-pedia.org/wiki/Short_QT_…

2022 brought further evidence of Nef-like mediated damage through Ana Pepe implicating the Tunneling Nanotube. Then, in 2023, came Akiko's paper.

2023 Apr 10
Enhanced inhibition of MHC-I expression by SARS-CoV-2 Omicron subvariants
Akiko Iwasaki
pmc.ncbi.nlm.nih.gov/articles/PMC10…

"We also included HIV Nef as a positive control for downregulating both MHC-I and MHC-II (30, 31), and SARS-CoV ORF8a/b proteins as a negative control."

Just published, further evidence of foam cell formation contributing to the development of atherosclerosis.
Published: 22 August 2025
SARS-CoV-2 infection induces pro-fibrotic and pro-thrombotic foam cell formation
nature.com/articles/s4156…

"Notably, infection by other coronaviruses tested, SARS-CoV-1, MERS-CoV and two bat coronaviruses (SHC014-CoV or WIV1-CoV), did not result in macrophage proliferation or foam cell formation."

That it isn't observed with other viruses can be explained by the protein appearing at citation #33 within the paper that appears at citation #33 above. Ironic, isn't?

"33. Eberhardt, N. et al. SARS-CoV-2 infection triggers pro-atherogenic inflammatory responses in human coronary vessels. Nat. Cardiovasc. Res."

Published: 28 September 2023
SARS-CoV-2 infection triggers pro-atherogenic inflammatory responses in human coronary vessels
nature.com/articles/s4416…

"33. Collins, K. L., Chen, B. K., Kalams, S. A., Walker, B. D. & Baltimore, D. HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes. Nature 391, 397–401 (1998)."

In 2024, this paper was published, specifically naming HIV's Tat.
SARS-CoV-2 targets ribosomal RNA biogenesis
cell.com/cell-reports/p…

"Finally, the Tat protein of human immunodeficiency virus interacts with FBL and U3 snoRNA, impairing prerRNA processing and depleting mature ribosomes."

See? Akiko told everyone they would develop and die from PAH.
Pathogenesis of HIV-Associated Pulmonary Hypertension
Potential Role of HIV-1 nef
pmc.ncbi.nlm.nih.gov/articles/PMC31…

"The HIV-PH mechanism remains unknown, but HIV proteins such as Tat and Nef may play a role. HIV-1 Nef is a broad-spectrum adaptor protein that may affect HIV-infected and uninfected pulmonary vascular cells."

"Among HIV proteins, Nef is the most strongly associated with HIV-related PH. Nef, a misnomer for “negative factor,” is expressed early during viral infection and is fundamental in HIV pathogenesis."

The aversion to addressing CD4 depletion, allowing the CD4 nadir, or lowest count, to drop has contributed to that which Harry mentions, Carotid Intima-Media Thickness.

That the hallmark of a chronic HIV infection is observed in another population was made known on September 28th, 2020.
SARS-CoV-2 Uses CD4 to Infect T Helper Lymphocytes
medrxiv.org/content/10.110…

Everyone was reminded it would be prudent to borrow from the concept of the Estimated Date of Seroconversion to prevent children from being concerned about their Estimated Date of what is an AIDS Death in March of 2022.

ACE2-independent infection of T lymphocytes by SARS-CoV-2
nature.com/articles/s4139…

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: Essentials of Diagnosis and Management
mayoclinicproceedings.org/article/S0025-…

"Although patients with ME/CFS do not present with symptomatic, opportunistic infections (eg, cryptosporidiosis, tuberculosis), some do experience recurrent herpes infections."

I would imagine embalming fluid will get rid of plaques.
2 students diagnosed with tuberculosis at Klein Cain High School
khou.com/article/news/l…

Since September of 2022, Covid-19 has appeared directly beside HIV on Merck Medical's website but we live among those who will borrow from human medicine to save Timmy's pet but not HIV medicine to save Timmy.

merckmanuals.com/professional/h…

Lymphocytopenia
Acquired lymphocytopenia
The most common causes include:
Protein-energy undernutrition
Infections, particularly viral (eg, HIV, COVID-19, EBV)
Autoimmune diseases
Malignancies
Cytotoxic or immunosuppressive medications

"Patients with COVID-19 also frequently have lymphocytopenia (35 to 83% of patients) (2). The cause of the lymphocytopenia due to COVID-19 is not completely understood, but COVID-19 can directly infect lymphocytes, likely leading to cytokine-related apoptosis of the cells."

"Lower lymphocyte counts portend a poor prognosis and an increased likelihood of requiring ICU admission and of dying from COVID-19."

The role of HIV Tat protein in HIV-related cardiovascular diseases
pmc.ncbi.nlm.nih.gov/articles/PMC59…

Same Tat. Same Corrected QT Interval Prolongation.
"Corrected QT (QTc) prolongation is predictive of cardiovascular mortality. Frequent occurrence of QT interval prolongation have been found in HIV-positive patients."

See?
"Carotid intima-media thickness (IMT), an indicator of generalized atherosclerosis, is higher in HIV patients than in age-matched control subjects, which is associated with classic coronary risk factors and with low CD4+ T cell count."

Did APOE4 appear on the list of genes associated with ME/CFS as it appears in SARS-Cov-2 and HIV literature? No.

SARS-CoV-2 infection of human brain microvascular endothelial cells leads to inflammatory activation through NF-κB non-canonical pathway and mitochondrial remodeling
pmc.ncbi.nlm.nih.gov/articles/PMC92…

We learned of Tight Junction destruction in December of 2020.
SARS-CoV-2 Envelope (E) Protein Interacts with PDZ-Domain-2 of Host Tight Junction Protein ZO1
pmc.ncbi.nlm.nih.gov/articles/PMC77…

Same Tat. Same brain microvascular endothelial cells. Same Tight Junction destruction.
Disruption of blood–brain barrier: effects of HIV Tat on brain microvascular endothelial cells and tight junction proteins
link.springer.com/article/10.100…

Are people who have long-haul COVID more at risk for Alzheimer’s? University researchers looking for a possible connection.
med.umn.edu/neurosurgery/n…

"It turns out that this subset of long-haul COVID sufferers often has a special genotype, according to Low. They have a gene known as APOE4, a variant of apolipoprotein that encodes proteins carrying cholesterol around the brain."

Speaking of cerebrovascular damage.
APOE ε4 associates with increased risk of severe COVID-19, cerebral microhaemorrhages and post-COVID mental fatigue: a Finnish biobank, autopsy and clinical study
actaneurocomms.biomedcentral.com/articles/10.11…

Same Tat. Same APOE4.
Apolipoprotein E isoform dependently affects Tat-mediated HIV-1 LTR transactivation
jneuroinflammation.biomedcentral.com/articles/10.11…

"Of the three ApoEs, ApoE4 was the least potent and effective at preventing HIV-1 Tat internalization and at decreasing Tat-mediated HIV-1 LTR transactivation."

Akiko causes arterial remodeling, atherosclerosis, and even Alzheimer's. Do those with ME/CFS experience elevation of phosphorylated tau-217? No.

There are those who read about cholesterol efflux pathway impairment and are planning on profiting off the silence of many.
FDA Clears First Blood Test Used in Diagnosing Alzheimer’s Disease
fda.gov/news-events/pr…

"The Lumipulse G pTau217/ß-Amyloid 1-42 Plasma Ratio is for the early detection of amyloid plaques associated with Alzheimer’s disease in adult patients, aged 55 years and older, exhibiting signs and symptoms of the disease."

See? See Nef Akiko used as a positive control. Same p-tau 217.
Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance
pubmed.ncbi.nlm.nih.gov/34843091/

Harry, listen to those living with ME/CFS and send more of your income source to the MEmory care unit or MorguE, you're doing great!