💉🧬🚨 DNA/RNA hybrids and R loops? Myself and others have been sharing concerns over these things. The impurities in the ionizable lipids CAN, theoretically do this with the formation of ADDUCTS. And you can test! Here's how:
"DNA adducts are formed when DNA undergoes chemical modifications. Usually, such chemical modifications are attributed to the attack on DNA by reactive chemical species"
In this case, we are addressing the reactive species that Moderna found in their lab, paid for by Moderna, where Moderna scientists found that the reactive species in the ionizable lipids were covalently bonding like super glue to the modified RNA that's used in the COVID RNA and LNP platform.
"DNA adductomics is mostly used as a tool to screen for DNA adducts, known or unknown, providing important evidence for exposure to specific carcinogens and/or related consequences, like cancer."
"DNA adducts are a form of DNA damage; if not repaired in time, they can interfere with DNA transcription and replication and induce mutations, contributing to carcinogenesis [12]. Thus, it is generally accepted that the formation of DNA adducts is the initial molecular event in carcinogenesis. Not surprisingly, most studies of DNA adducts are related to genotoxicity and mutagenicity/carcinogenicity."
"DNA adducts can be categorized into monoadducts and crosslinks. The former are the adducts formed through chemical modifications by small molecules on individual nucleobases. However, if the modifications of nucleobases occur by reacting with macromolecules, such as proteins and RNA, the adducts formed are classified as crosslinks, i.e., DNA-protein crosslinks (DPCs) and DNA-RNA crosslinks,"
These are quote from the article that I will link below.
👀What the heck is a cross link?
A crosslink is a covalent bond between two nucleic acids that normally shouldn’t be linked ( DNA-DNA, DNA-RNA, RNA-protein).
If you are reading and if you read this post below or watched my presentation with Dr McMillan, then you know that the ionizable lipids have a positive charge and they are attracted to the negative charge on the backbone of the DNA, which can be the DNA plasmid to contamination OR our HUMAN DNA, the modified RNA in the COVID RNA LNP platform, OR, OUR HUMAN RNA, or it can link up and bond to proteins, like superglue.
They are more damaging than a simple monoadduct (just linking up to one thing) because they physically block transcription and replication machinery (polymerases, helicases, splicing complexes).
It's making a sandwich! For lack of a better phrase, the adducts might also be creating sandwiches because there are different spots on the lipids that can react! It's like looking at an Oreo cookie and the inside of the Oreo is the positively charged lipid and on either side maybe you have a piece of DNA and the other side is a piece of RNA, except it's not a cookie you want to eat.
This is a big deal in cancer drugs because chemo drugs (like nitrogen mustards) deliberately induce crosslinks: they jam the replication machinery, killing dividing cancer cells. But this can also cause cancer.
DNA plasmid contaminants (linearized dsDNA plasmid) could, in theory, mispair or form abnormal hybrid regions with cellular RNA, especially if they carry unmethylated CpG islands or secondary structures.
RNA (spike mRNA) could base-pair with cellular DNA (in R-loops) or with plasmid DNA, creating DNA-RNA hybrids, and THERE is the plausible mechanism if other scientists are proposing or seeing what could be the spike modified RNA gluing itself to the plasmid DNA or, the potential to do this in the human body!
These hybrids are unstable and can be chemically crosslinked if reactive species (oxidative stress, aldehydes, lipid byproducts from LNPs) are present, and guess what? Moderna proved they ARE present!
🚨🔬 I'm going to add these sections to the pre-print in a more structured, scientific form meant for a journal where it belongs, not just a preprint.
Crosslinks block transcription of pri-miRNAs, so certain miRNAs go down. This also means that if you wanted to test someone, besides just getting a hold of these positively charged lipids or some vials of Pfizer and moderna, and looking for adducts in blood or in tissues of animals after injecting them using mass spec and reverse phase ion pair HPLC, you could also scan for these effects downstream.
One of the things you might see is the impact on microRNAs.
DNA damage response pathways (ATM/ATR, p53) might activate or repress miRNAs as part of the repair process.
👀 What about dicer?
Dicer is a RNase III family endonuclease that plays a central role in the microRNA (miRNA) and small interfering RNA (siRNA) biogenesis pathway.
Dicer is mostly cytoplasmic, though it can shuttle and has been detected in nuclei under some conditions.
Size: In humans, Dicer is a large 200 kDa multidomain protein.
Dicer processes precursor RNAs into short ~21–25 nucleotide duplex RNAs with defined ends.
Dicer mutations or dysfunction are associated with cancer, neurodegeneration, infertility, and autoimmune conditions.
Dicer itself can be post-translationally modified (phosphorylation, cleavage by caspases under apoptosis, oxidative modifications).
Now we have the concern over ADDUCTS interacting in the cells with microRNAs, which, downstream, if you've get a mutation in DICER, that might lead to trouble.
DNA damage or chronic immune activation can downregulate Dicer transcriptionally, producing long-lasting changes in miRNA expression.
Adduction of RNA-binding proteins or processing enzymes (Drosha, Dicer, Exportin-5) could dysregulate global miRNA biogenesis, and this, while not a BLUNT hit, can lead to disease, and studies show this.
Most miRNA responses to acute insults are transient (hours–weeks). Durable changes can occur, but are less common and usually require persistent stimulus or epigenetic rewiring. It would be a bit difficult to track those events unless it was right after injection. Some animal studies might be in order!
But tracking mutation due to an adduct is a different story!!!
These are specific.
I don't have a lab, And I'm just a single lady typing here on a Saturday night (I get asked out by creatins and I say no) because I don't have anyone to talk to about this, AND, I know some of you reading are scientists and you have labs! I have a lab nearby I can rent that's a full lab that's an extension of my former University, but I currently don't have the resources to do that as I'm not affiliated.
👀🔬💉Adduct-specific mutational signatures ARE REAL. Many genotoxins leave characteristic patterns of base substitutions ( tobacco-linked C>A). Whole-genome sequencing (WGS) of affected cells/tumors could reveal an excess of a specific substitution pattern matching in vitro mutational spectra produced by the electrophile AND in this case, we are talking about the impurities in the lipids interacting with DNA.
Do not go out and do any of this unless you are scientist. None of this is meant to treat or diagnose.
However, if you are a scientist currently testing tissue in a lab somewhere under IRB protocol where you have solid IRB protocol in place and you have followed all the rules, and someone with tissue from a biopsy from a tumor was sent, then clonal expansion bearing the mutation that arises post-exposure could be tracked in longitudinal samples, if available. This would be different than if you were checking using PCR or some other pattern. You would see this adduct pattern in the mutation, like in colon cancer, for example. This could be present with or without the DNA plasmid. You could have both. The absence of DNA plasmid in tissue samples does not denote the absence of possible mutation after receiving a COVID RNA LNP "vaccine". PCR is not the end-all be all of tests.
The DNA plasmid has been something that many people have been checking vials for and thank goodness for that. Additionally perhaps scientists who still have some of this leftover should check the vials for the impurities. You're going to see something.
If you're looking for the microRNAs and the impact on those, here are some potential tests. I'm not currently sitting in a lab and if you are you may have better ideas.
Uses an antibody (S9.6) to pull down DNA-RNA hybrids.
If plasmid contamination or spike RNA is contributing to hybrid formation, DRIP-seq would possibly light it up.
Transcriptomic fingerprints (microRNAs, mRNA splicing errors):
Dicer and Argonaute processing can get “jammed” when crosslinks interfere with normal transcription.
You’d see aberrant miRNA profiles, especially those involved in DNA damage response (miR-34, miR-155, miR-16 families).
For the summary, the concern is that the impurities in the positively charged ionizable lipids and those that gain a charge maybe behind, in part, adduct formation that can lead to a whole host of problems, not including forming adducts with our own DNA, the DNA plasmid in the COVID vaccines, forming addicts with the modified RNA inside them, and when they hit the inside of cells and tissues, the very real potential adducts are forming there with our own nucleic acids, with our own RNA, our proteins and our DNA.
Those lipids are entering per dose at rates into the quadrillions if we think of the individual pieces of modified RNA per dose being approximately 43 trillion. And even if 1% of those react we're going to have concerns and we're not just talking in the arm or liver these things travel head to toe.
Then those lipids could possibly be in part behind the DNA RNA cross linking/r loops, and then concerns with microRNA expression.
Hope not.
"DNA adducts are formed when DNA undergoes chemical modifications. Usually, such chemical modifications are attributed to the attack on DNA by reactive chemical species"
In this case, we are addressing the reactive species that Moderna found in their lab, paid for by Moderna, where Moderna scientists found that the reactive species in the ionizable lipids were covalently bonding like super glue to the modified RNA that's used in the COVID RNA and LNP platform.
"DNA adductomics is mostly used as a tool to screen for DNA adducts, known or unknown, providing important evidence for exposure to specific carcinogens and/or related consequences, like cancer."
"DNA adducts are a form of DNA damage; if not repaired in time, they can interfere with DNA transcription and replication and induce mutations, contributing to carcinogenesis [12]. Thus, it is generally accepted that the formation of DNA adducts is the initial molecular event in carcinogenesis. Not surprisingly, most studies of DNA adducts are related to genotoxicity and mutagenicity/carcinogenicity."
"DNA adducts can be categorized into monoadducts and crosslinks. The former are the adducts formed through chemical modifications by small molecules on individual nucleobases. However, if the modifications of nucleobases occur by reacting with macromolecules, such as proteins and RNA, the adducts formed are classified as crosslinks, i.e., DNA-protein crosslinks (DPCs) and DNA-RNA crosslinks,"
These are quote from the article that I will link below.
👀What the heck is a cross link?
A crosslink is a covalent bond between two nucleic acids that normally shouldn’t be linked ( DNA-DNA, DNA-RNA, RNA-protein).
If you are reading and if you read this post below or watched my presentation with Dr McMillan, then you know that the ionizable lipids have a positive charge and they are attracted to the negative charge on the backbone of the DNA, which can be the DNA plasmid to contamination OR our HUMAN DNA, the modified RNA in the COVID RNA LNP platform, OR, OUR HUMAN RNA, or it can link up and bond to proteins, like superglue.
They are more damaging than a simple monoadduct (just linking up to one thing) because they physically block transcription and replication machinery (polymerases, helicases, splicing complexes).
It's making a sandwich! For lack of a better phrase, the adducts might also be creating sandwiches because there are different spots on the lipids that can react! It's like looking at an Oreo cookie and the inside of the Oreo is the positively charged lipid and on either side maybe you have a piece of DNA and the other side is a piece of RNA, except it's not a cookie you want to eat.
This is a big deal in cancer drugs because chemo drugs (like nitrogen mustards) deliberately induce crosslinks: they jam the replication machinery, killing dividing cancer cells. But this can also cause cancer.
DNA plasmid contaminants (linearized dsDNA plasmid) could, in theory, mispair or form abnormal hybrid regions with cellular RNA, especially if they carry unmethylated CpG islands or secondary structures.
RNA (spike mRNA) could base-pair with cellular DNA (in R-loops) or with plasmid DNA, creating DNA-RNA hybrids, and THERE is the plausible mechanism if other scientists are proposing or seeing what could be the spike modified RNA gluing itself to the plasmid DNA or, the potential to do this in the human body!
These hybrids are unstable and can be chemically crosslinked if reactive species (oxidative stress, aldehydes, lipid byproducts from LNPs) are present, and guess what? Moderna proved they ARE present!
🚨🔬 I'm going to add these sections to the pre-print in a more structured, scientific form meant for a journal where it belongs, not just a preprint.
Crosslinks block transcription of pri-miRNAs, so certain miRNAs go down. This also means that if you wanted to test someone, besides just getting a hold of these positively charged lipids or some vials of Pfizer and moderna, and looking for adducts in blood or in tissues of animals after injecting them using mass spec and reverse phase ion pair HPLC, you could also scan for these effects downstream.
One of the things you might see is the impact on microRNAs.
DNA damage response pathways (ATM/ATR, p53) might activate or repress miRNAs as part of the repair process.
👀 What about dicer?
Dicer is a RNase III family endonuclease that plays a central role in the microRNA (miRNA) and small interfering RNA (siRNA) biogenesis pathway.
Dicer is mostly cytoplasmic, though it can shuttle and has been detected in nuclei under some conditions.
Size: In humans, Dicer is a large 200 kDa multidomain protein.
Dicer processes precursor RNAs into short ~21–25 nucleotide duplex RNAs with defined ends.
Dicer mutations or dysfunction are associated with cancer, neurodegeneration, infertility, and autoimmune conditions.
Dicer itself can be post-translationally modified (phosphorylation, cleavage by caspases under apoptosis, oxidative modifications).
Now we have the concern over ADDUCTS interacting in the cells with microRNAs, which, downstream, if you've get a mutation in DICER, that might lead to trouble.
DNA damage or chronic immune activation can downregulate Dicer transcriptionally, producing long-lasting changes in miRNA expression.
Adduction of RNA-binding proteins or processing enzymes (Drosha, Dicer, Exportin-5) could dysregulate global miRNA biogenesis, and this, while not a BLUNT hit, can lead to disease, and studies show this.
Most miRNA responses to acute insults are transient (hours–weeks). Durable changes can occur, but are less common and usually require persistent stimulus or epigenetic rewiring. It would be a bit difficult to track those events unless it was right after injection. Some animal studies might be in order!
But tracking mutation due to an adduct is a different story!!!
These are specific.
I don't have a lab, And I'm just a single lady typing here on a Saturday night (I get asked out by creatins and I say no) because I don't have anyone to talk to about this, AND, I know some of you reading are scientists and you have labs! I have a lab nearby I can rent that's a full lab that's an extension of my former University, but I currently don't have the resources to do that as I'm not affiliated.
👀🔬💉Adduct-specific mutational signatures ARE REAL. Many genotoxins leave characteristic patterns of base substitutions ( tobacco-linked C>A). Whole-genome sequencing (WGS) of affected cells/tumors could reveal an excess of a specific substitution pattern matching in vitro mutational spectra produced by the electrophile AND in this case, we are talking about the impurities in the lipids interacting with DNA.
Do not go out and do any of this unless you are scientist. None of this is meant to treat or diagnose.
However, if you are a scientist currently testing tissue in a lab somewhere under IRB protocol where you have solid IRB protocol in place and you have followed all the rules, and someone with tissue from a biopsy from a tumor was sent, then clonal expansion bearing the mutation that arises post-exposure could be tracked in longitudinal samples, if available. This would be different than if you were checking using PCR or some other pattern. You would see this adduct pattern in the mutation, like in colon cancer, for example. This could be present with or without the DNA plasmid. You could have both. The absence of DNA plasmid in tissue samples does not denote the absence of possible mutation after receiving a COVID RNA LNP "vaccine". PCR is not the end-all be all of tests.
The DNA plasmid has been something that many people have been checking vials for and thank goodness for that. Additionally perhaps scientists who still have some of this leftover should check the vials for the impurities. You're going to see something.
If you're looking for the microRNAs and the impact on those, here are some potential tests. I'm not currently sitting in a lab and if you are you may have better ideas.
Uses an antibody (S9.6) to pull down DNA-RNA hybrids.
If plasmid contamination or spike RNA is contributing to hybrid formation, DRIP-seq would possibly light it up.
Transcriptomic fingerprints (microRNAs, mRNA splicing errors):
Dicer and Argonaute processing can get “jammed” when crosslinks interfere with normal transcription.
You’d see aberrant miRNA profiles, especially those involved in DNA damage response (miR-34, miR-155, miR-16 families).
For the summary, the concern is that the impurities in the positively charged ionizable lipids and those that gain a charge maybe behind, in part, adduct formation that can lead to a whole host of problems, not including forming adducts with our own DNA, the DNA plasmid in the COVID vaccines, forming addicts with the modified RNA inside them, and when they hit the inside of cells and tissues, the very real potential adducts are forming there with our own nucleic acids, with our own RNA, our proteins and our DNA.
Those lipids are entering per dose at rates into the quadrillions if we think of the individual pieces of modified RNA per dose being approximately 43 trillion. And even if 1% of those react we're going to have concerns and we're not just talking in the arm or liver these things travel head to toe.
Then those lipids could possibly be in part behind the DNA RNA cross linking/r loops, and then concerns with microRNA expression.
Hope not.
Here are how those R loops that some of your guests and other scientists have raised concerns about most likely are forming from the 💉!
@drdrew
@DrTeckKhong
@DrAseemMalhotra
@DrJohnB2
@DrMakaryFDA
@RobertKennedyJr
@RWMaloneMD
@jordanbpeterson
@BretWeinstein
@RennickGBR
@ClareCraigPath
@drdrew
@DrTeckKhong
@DrAseemMalhotra
@DrJohnB2
@DrMakaryFDA
@RobertKennedyJr
@RWMaloneMD
@jordanbpeterson
@BretWeinstein
@RennickGBR
@ClareCraigPath
• • •
Missing some Tweet in this thread? You can try to
force a refresh
