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Anders Sejr Hansen Profile picture
Anders Sejr Hansen
@Anders_S_Hansen
Associate Professor @MITdeptofBE : https://t.co/uy48t7k949 Interested in understanding the relationship between 3D genome structure and function
Apr 14, 2022 • 6 tweets • 13 min read
(1/6) our preprint biorxiv.org/content/10.110… is now out in @ScienceMagazine science.org/doi/10.1126/sc…

Using super-res live-imaging we show that CTCF/cohesin loops are dynamic (~10-30 min median lifetime), rare (~3-6.5% looped fraction), and we fully parameterize a TAD. (2/6) Thanks to @dijiang319 and to the reviewers for a thoughtful peer-review process.

A full tweetorial summarizing the preprint can be found below:


Since then @LucaGiorgetti lab has a new preprint with similar results:
biorxiv.org/content/10.110…
Dec 14, 2021 • 11 tweets • 30 min read
(1/n) excited to share our new preprint:

We visualize and track a CTCF/cohesin loop live using super-res live-cell imaging.

CTCF/cohesin loop is rare (~3-6.5% of time) and very dynamic (~10-30 min lifetime), ruling out models of TADs as stable loops.

biorxiv.org/content/10.110… @labronic_mike @hugoresearch @SGrosseHolz @AsmitaJha16 @CCattoglio @Stanley_TH @leonidmirny @zechnerlab @hansen_lab @MITdeptofBE (2/n) We focus on: Fbn2 TAD in mESC since simple and verified by @deWitLab to be CTCF-dependent.

Using genome-editing, we fluorescently label the 2 CTCF anchors (C36) and generate pos and neg looping controls (C27 and C65).

Micro-C verifies that tagging does not perturb loop