Mostly #cryoEM-related tweets. Group leader at a biotech company. Views expressed are strictly my own.
Jun 6, 2020 • 17 tweets • 7 min read
biorxiv.org/content/10.110…
In this Mmmanuscript we use our new tool M to break a couple of things, as is customary in #cryoEM. Most importantly, we show that 3.7 Å resolution can be achieved for a protein directly inside a cell. Software release next week. Now, thread:
M performs multi-particle refinement. One of single-particle analysis' central assumptions is that the particles are, in fact, single. This is physically never the case, and there is benefit in treating all items in a micrograph/tomogram as a connected system.
