Our latest pre-print, exploring the ways 3C methods can be improved to generate high-resolution interaction profiles for 1000’s of genes, is out now!

#generegulation #3Dgenome

(key findings below in emojis)

biorxiv.org/content/10.110… Using a mixture of human and mouse cells we found up to 30% of ligations in standard in situ 3C libraries are BETWEEN nuclei! 😱🔊😱

As well as developing 3C techniques we develop software to design and analyse 3C experiments. We’ve put together a suite of tools “The Capture Compendium” to take you from idea to publication. The pre-print is now on bioRxiv (1/7)

biorxiv.org/content/10.110… Capsequm2 with easy filtering on AltSort helps you design probes to target your region of interest. (2/7)

apps.molbiol.ox.ac.uk/CaptureC/cgi-b…

In our latest pre-print a talented PhD student (Martin Larke) developed a new method, scaRNA-seq, to examine the role of enhancers in regulating transcription initation and pausing.

biorxiv.org/content/10.110… scaRNAseq (Think Simba's uncle) enriches for single short (<300nt) capped RNA molecules. Using it you can simultaneously identify transcription start and pause sites.

We're very proud to finally share our work tackling the best way to decode GWAS hits.

biorxiv.org/cgi/content/sh… 1) We use the fundemental regulatory building blocks, promoters, ehancers, and boundaries, to identify key cell types.