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Luciano Martelotto 🛠🧬💻🇦🇺 Profile picture
Luciano Martelotto 🛠🧬💻🇦🇺
@LGMartelotto
Sc/St-Omics pro-aficionado. Knowledge sharing is my thing. Views, opinions and comments are my own and do not represent those of my employer. Like/RT ≠ endorse
Jun 28, 2022 • 4 tweets • 3 min read
We wondered what a side-to-side comparison between 3’ v3.1 & RNA Fix solution from @10xGenomics would look like? Well, we have WINNER!
I said it two #10Xperiences ago…this kit is by far the best these guys have ever produced yet! @afvallejop @JensDurruthy @DrJasPlummer @gmcderm ImageImage And as my postdoc CompBio superstar @afvallejop said…one more for the road Image
Nov 12, 2021 • 6 tweets • 2 min read
Ok, we did try @ParseBio SPLIT-Seq WT kit. We don’t have the data yet but workflow-wise it was super easy. I really liked the simplicity and that the kit comes with pretty much all you need. Totally manageable for anybody who has done 96-well work and multi-channel pipettes. 1/6 They also have a super easy to fill out Excel sheet that makes all the calculations for you. Peace of mind right there. If you counted your cells correctly then you are for a good start. 2/6
Feb 3, 2020 • 17 tweets • 10 min read
Dear #singlecell global team (#scGT), it was a very inspiring week for #scQA. @hoheyn and I would like to THANK to the @cmcginnisUCSF for his comprehensive Tweetorial on #MultiSeq.

For the Feb3/2020 #scQA/#scQC Forum (w/@hoheyn &
@LGMartelotto) we'll discuss some #snATAC-Seq! First a bit of history...

Assay for Transposase-Accessible Chromatin (ATAC-Seq) was originally published in in 2013 (@naturemethods PMID: 24097267) by @JD_Buenrostro in the labs of H. Chang and W. Greenleaf at @Stanford.