Share this page!

Enter URL or ID to Unroll

You can paste full URL like: https://x.com/threadreaderapp/status/1644127596119195649
or just the ID like: 1644127596119195649

How to get URL link on X (Twitter) App

  1. On the Twitter thread, click on or icon on the bottom
  2. Click again on or Share Via icon
  3. Click on Copy Link to Tweet
  4. Paste it above and click "Unroll Thread"!
  5. More info at Twitter Help
Liz Tseng Profile picture
Liz Tseng
@Magdoll
Applications product manager @PacBio. #RNA/#GeneTherapy/#bioinformatics. @uwcse PhD. she/her. Opinions are my own. also: @magdoll.bsky.social
Feb 25 • 12 tweets • 4 min read
If you're studying #bioinformatics, there comes a time when you have to decide between:

- staying in academia
- going into industry
- give up #bioinformtics and do something else

Well, let me tell you what it was like for me to work as a #bioinformatics in the biotech industry! /1Image 2/ Before I became a product manager at @PacBio (topic for another day) I was a hired as a #bioinformatics scientist. I had graduated with PhD in computer science from @UW. UW doesn't have a bfx program but a cross-department Computational Molecular Biology certificate program grad.uw.edu/programs/inter…
Jun 9, 2022 • 11 tweets • 5 min read
#bioinformatics tip of the day: FOLLOW THE DATA. Trace a read through its journey. Example is when I look at a fresh @PacBio Iso-Seq run (but really for any sequencing data for any application).

I start w CCS reads, scroll through them to viz the primers and polyA tail. /1 @PacBio 2/ I run lima to strip the cDNA primers, check the summary file to see a good percentage actually has the expected 5'/3' cDNA primers. if a high % failed, look for reasons why - is it user error (wrong primers?), prep error, or biological?